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Plasma redox status is impaired in the portacaval shunted rat--the risk of the reduced antioxidant ability.

Aller MA, García-Fernández MI, Sánchez-Patán F, Santín L, Rioja J, Anchuelo R, Arias J, Arias JL - Comp Hepatol (2008)

Bottom Line: Plasma levels of the oxidant serum advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), the antioxidant total thiol (GSH) and total antioxidant status (TAX) were measured.Albumin, ammonia, Aspartate-aminotransferase (AST), Alanine-aminotransferase (ALT), thiostatin and alpha-1-acid glycoprotein (alpha1-AGP) were also assayed 4 weeks after the operation.It could be hypothesized, therefore, that the reduced antioxidant defences would mediate a systemic inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Surgery I Department, School of Medicine, Complutense University of Madrid, Spain. maaller@med.ucm.es

ABSTRACT

Background: Portacaval shunting in rats produces a reduction of hepatic oxidant scavenging ability. Since this imbalance in hepatic oxidant/antioxidant homeostasis could coexist with systemic changes of oxidant stress/antioxidant status, plasma oxidants and antioxidant redox status in plasma of portacaval shunted-rats were determined.

Results: Male Wistar male: Control (n = 11) and with portacaval shunt (PCS; n = 11) were used. Plasma levels of the oxidant serum advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), the antioxidant total thiol (GSH) and total antioxidant status (TAX) were measured. Albumin, ammonia, Aspartate-aminotransferase (AST), Alanine-aminotransferase (ALT), thiostatin and alpha-1-acid glycoprotein (alpha1-AGP) were also assayed 4 weeks after the operation. AOPPs were significantly higher (50.51 +/- 17.87 vs. 36.25 +/- 7.21 microM; p = 0.02) and TAX was significantly lower (0.65 +/- 0.03 vs. 0.73 +/- 0.06 mM; p = 0.007) in PCS compared to control rats. Also, there was hypoalbuminemia (2.54 +/- 0.08 vs. 2.89 +/- 0.18 g/dl; p = 0.0001) and hyperammonemia (274.00 +/- 92.25 vs. 104.00 +/- 48.05 microM; p = 0.0001) and an increase of thiostatin (0.23 +/- 0.04 vs. 0.09 +/- 0.01 mg/ml; p = 0.001) in rats with a portacaval shunt. The serum concentration of ammonia is correlated with albumin levels (r = 0.624; p = 0.04) and TAX correlates with liver weight (r = 0.729; p = 0.017) and albumin levels (r = 0.79; p = 0.007)

Conclusion: These findings suggest that in rats with a portacaval shunt a systemic reduction of oxidant scavenging ability, correlated with hyperammonemia, is principally produced. It could be hypothesized, therefore, that the reduced antioxidant defences would mediate a systemic inflammation.

No MeSH data available.


Related in: MedlinePlus

Redox status in control rats and in rats with portacaval shunt at 4 weeks of evolution. Duplicate (TAX, AOPP and THIOLS) and triplicate (LOOH) assays in control (n = 11) and portocaval shunt (PCS) (n = 11) rats, except for TAX in which one PCS value was excluded. The results are expressed as mean ± SD. AOPP: serum advanced oxidation protein product; LOOH: serum lipid hydroperoxides; TAX: serum total antioxidant; THIOLS: total plasma thiols.
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Figure 1: Redox status in control rats and in rats with portacaval shunt at 4 weeks of evolution. Duplicate (TAX, AOPP and THIOLS) and triplicate (LOOH) assays in control (n = 11) and portocaval shunt (PCS) (n = 11) rats, except for TAX in which one PCS value was excluded. The results are expressed as mean ± SD. AOPP: serum advanced oxidation protein product; LOOH: serum lipid hydroperoxides; TAX: serum total antioxidant; THIOLS: total plasma thiols.

Mentions: The serum advanced oxidation protein product (AOPP) level increases (p = 0.02) whereas total antioxidant status (TAX) decreases (p = 0.007) in portacaval shunted rats in relation to control rats. The serum concentrations of lipid hydroperoxides (LOOH) and total thiols do not change in PCS-rats (Figure 1).


Plasma redox status is impaired in the portacaval shunted rat--the risk of the reduced antioxidant ability.

Aller MA, García-Fernández MI, Sánchez-Patán F, Santín L, Rioja J, Anchuelo R, Arias J, Arias JL - Comp Hepatol (2008)

Redox status in control rats and in rats with portacaval shunt at 4 weeks of evolution. Duplicate (TAX, AOPP and THIOLS) and triplicate (LOOH) assays in control (n = 11) and portocaval shunt (PCS) (n = 11) rats, except for TAX in which one PCS value was excluded. The results are expressed as mean ± SD. AOPP: serum advanced oxidation protein product; LOOH: serum lipid hydroperoxides; TAX: serum total antioxidant; THIOLS: total plasma thiols.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2262055&req=5

Figure 1: Redox status in control rats and in rats with portacaval shunt at 4 weeks of evolution. Duplicate (TAX, AOPP and THIOLS) and triplicate (LOOH) assays in control (n = 11) and portocaval shunt (PCS) (n = 11) rats, except for TAX in which one PCS value was excluded. The results are expressed as mean ± SD. AOPP: serum advanced oxidation protein product; LOOH: serum lipid hydroperoxides; TAX: serum total antioxidant; THIOLS: total plasma thiols.
Mentions: The serum advanced oxidation protein product (AOPP) level increases (p = 0.02) whereas total antioxidant status (TAX) decreases (p = 0.007) in portacaval shunted rats in relation to control rats. The serum concentrations of lipid hydroperoxides (LOOH) and total thiols do not change in PCS-rats (Figure 1).

Bottom Line: Plasma levels of the oxidant serum advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), the antioxidant total thiol (GSH) and total antioxidant status (TAX) were measured.Albumin, ammonia, Aspartate-aminotransferase (AST), Alanine-aminotransferase (ALT), thiostatin and alpha-1-acid glycoprotein (alpha1-AGP) were also assayed 4 weeks after the operation.It could be hypothesized, therefore, that the reduced antioxidant defences would mediate a systemic inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Surgery I Department, School of Medicine, Complutense University of Madrid, Spain. maaller@med.ucm.es

ABSTRACT

Background: Portacaval shunting in rats produces a reduction of hepatic oxidant scavenging ability. Since this imbalance in hepatic oxidant/antioxidant homeostasis could coexist with systemic changes of oxidant stress/antioxidant status, plasma oxidants and antioxidant redox status in plasma of portacaval shunted-rats were determined.

Results: Male Wistar male: Control (n = 11) and with portacaval shunt (PCS; n = 11) were used. Plasma levels of the oxidant serum advanced oxidation protein products (AOPP), lipid hydroperoxides (LOOH), the antioxidant total thiol (GSH) and total antioxidant status (TAX) were measured. Albumin, ammonia, Aspartate-aminotransferase (AST), Alanine-aminotransferase (ALT), thiostatin and alpha-1-acid glycoprotein (alpha1-AGP) were also assayed 4 weeks after the operation. AOPPs were significantly higher (50.51 +/- 17.87 vs. 36.25 +/- 7.21 microM; p = 0.02) and TAX was significantly lower (0.65 +/- 0.03 vs. 0.73 +/- 0.06 mM; p = 0.007) in PCS compared to control rats. Also, there was hypoalbuminemia (2.54 +/- 0.08 vs. 2.89 +/- 0.18 g/dl; p = 0.0001) and hyperammonemia (274.00 +/- 92.25 vs. 104.00 +/- 48.05 microM; p = 0.0001) and an increase of thiostatin (0.23 +/- 0.04 vs. 0.09 +/- 0.01 mg/ml; p = 0.001) in rats with a portacaval shunt. The serum concentration of ammonia is correlated with albumin levels (r = 0.624; p = 0.04) and TAX correlates with liver weight (r = 0.729; p = 0.017) and albumin levels (r = 0.79; p = 0.007)

Conclusion: These findings suggest that in rats with a portacaval shunt a systemic reduction of oxidant scavenging ability, correlated with hyperammonemia, is principally produced. It could be hypothesized, therefore, that the reduced antioxidant defences would mediate a systemic inflammation.

No MeSH data available.


Related in: MedlinePlus