Limits...
Ran sticks a GEF to chromatin

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Gradients of the small GTPase Ran are important throughout the cell cycle... High nuclear RanGTP concentrations in interphase cells regulate nuclear transport... During mitosis, chromosome-localized RanGTP releases spindle assembly factors from sequestration by importins... On page 635, Li et al. demonstrate that RanGTP accumulates at chromosomes because the complex of Ran and its guanine nucleotide exchange factor (GEF) binds strongly to chromatin... GTP-bound Ran is produced by the GEF RCC1, which binds only weakly to histones... As RCC1 has strong GEF activity in the presence or absence of chromatin, the authors were interested in determining how RanGTP production is limited to chromosomes... Only nucleotide exchange, which displaces RCC1 from Ran, released the GEF and RanGTP from chromosomes... The increased affinity of the complex for chromosomes can be explained by its binding geometry... RCC1 binds histones H2A and H2B, whereas Ran binds weakly to histones H3 and H4... The complex is therefore well suited to bind to nucleosome octamers... Thus, exploiting the geometry of nucleosomes is a simple way to couple GTP exchange to chromatin-bound Ran. ▪

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Related in: MedlinePlus

Ran-bound RCC1 (bottom) is less mobile (circle, left to right) at chromosomes than is unbound RCC1 (top).
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uro1: Ran-bound RCC1 (bottom) is less mobile (circle, left to right) at chromosomes than is unbound RCC1 (top).


Ran sticks a GEF to chromatin
Ran-bound RCC1 (bottom) is less mobile (circle, left to right) at chromosomes than is unbound RCC1 (top).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2255590&req=5

uro1: Ran-bound RCC1 (bottom) is less mobile (circle, left to right) at chromosomes than is unbound RCC1 (top).

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Gradients of the small GTPase Ran are important throughout the cell cycle... High nuclear RanGTP concentrations in interphase cells regulate nuclear transport... During mitosis, chromosome-localized RanGTP releases spindle assembly factors from sequestration by importins... On page 635, Li et al. demonstrate that RanGTP accumulates at chromosomes because the complex of Ran and its guanine nucleotide exchange factor (GEF) binds strongly to chromatin... GTP-bound Ran is produced by the GEF RCC1, which binds only weakly to histones... As RCC1 has strong GEF activity in the presence or absence of chromatin, the authors were interested in determining how RanGTP production is limited to chromosomes... Only nucleotide exchange, which displaces RCC1 from Ran, released the GEF and RanGTP from chromosomes... The increased affinity of the complex for chromosomes can be explained by its binding geometry... RCC1 binds histones H2A and H2B, whereas Ran binds weakly to histones H3 and H4... The complex is therefore well suited to bind to nucleosome octamers... Thus, exploiting the geometry of nucleosomes is a simple way to couple GTP exchange to chromatin-bound Ran. ▪

No MeSH data available.


Related in: MedlinePlus