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A penetrating ocular injury can affect the induction of anterior chamber-associated immune deviation.

Lei F, Zhang J, Zhang J, He H, Du Y, Yang P - Mol. Vis. (2008)

Bottom Line: However, the levels of IFN-gamma produced by splenocytes of the AC-injection group and the 72-h and 120-h group were significantly lower than those in the 24-h and 48-h group.An increased frequency of CD4(+)CD25(+)Foxp3(+)T cells was found in the AC-injection group and the 72-h and 120-h group.Penetrating ocular injury preformed shortly (24 h-48 h) after an AC injection of an antigen was able to abrogate ACAID and was associated with a decreased production of TGF-beta1 and IL-10, an increased production of IFN-gamma, and a decreased expression of CD4(+)CD25(+)Foxp3(+)T cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First Affiliated Hospital, Department of Microbiology and Immunology, Zheng Zhou University, Zhengzhou, Henan Province, China.

ABSTRACT

Purpose: To determine the effect of penetrating ocular injury on the induction of anterior chamber-associated immune deviation (ACAID).

Methods: An injection of 5 mul ovalbumin (OVA, 20 mg/ml) into the anterior chamber (AC) of female BALB/c mice was performed to induce ACAID. A penetrating ocular injury was induced via the limbus on OVA-inoculated eyes at 24 h, 48 h, 72 h, and 120 h following AC injection. The mice receiving an OVA inoculation without the ocular injury served as the AC-injection group. Delayed type hypersensitivity (DTH) was examined to evaluate the induction of ACAID. The levels of transforming growth factor (TGF)-beta1, interleukin (IL)-10, and interferon (IFN)-gamma produced by splenocytes were detected by enzyme-linked immunosorbent assays (ELISA). The frequency of CD4(+)CD25(+)Foxp3(+)T cells in the splenocytes was detected by flow cytometry.

Results: A significantly decreased DTH response was observed in the AC-injection group as well as in mice that received a penetrating injury at 72 h and 120 h following AC-injection of OVA. The levels of TGF-beta1 and IL-10 produced by splenocytes of mice in the AC-injection group and in the 72-h and 120-h group were significant higher than those in the 24-h and 48-h group. However, the levels of IFN-gamma produced by splenocytes of the AC-injection group and the 72-h and 120-h group were significantly lower than those in the 24-h and 48-h group. An increased frequency of CD4(+)CD25(+)Foxp3(+)T cells was found in the AC-injection group and the 72-h and 120-h group.

Conclusions: Penetrating ocular injury preformed shortly (24 h-48 h) after an AC injection of an antigen was able to abrogate ACAID and was associated with a decreased production of TGF-beta1 and IL-10, an increased production of IFN-gamma, and a decreased expression of CD4(+)CD25(+)Foxp3(+)T cells.

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Detection of TGF-β1 produced by splenocyte. Mice were sacrificed seven days after s.c. immunization. Spleens were removed and grinded to prepare a single-cell suspension. These cells were incubated in a complete RPMI 1640 medium with OVA (100 μg/ml) for 48 h in 24-well culture plates (2×106 cells/well). Supernatants were collected for determining the quantity of TGF-β1 by ELISA. Data are the mean ± SEM (n=6 per group). The asterisk indicates that p<0.05 as compared with the positive control.
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f3: Detection of TGF-β1 produced by splenocyte. Mice were sacrificed seven days after s.c. immunization. Spleens were removed and grinded to prepare a single-cell suspension. These cells were incubated in a complete RPMI 1640 medium with OVA (100 μg/ml) for 48 h in 24-well culture plates (2×106 cells/well). Supernatants were collected for determining the quantity of TGF-β1 by ELISA. Data are the mean ± SEM (n=6 per group). The asterisk indicates that p<0.05 as compared with the positive control.

Mentions: As cytokines, such as TGF-β1, IL-10, and IFN-γ, were involved in the induction of ACAID [21-25], our study examined whether there was an alteration in the production of these cytokines in the penetrating ocular injury groups. As shown in Figure 3, Figure 4, and Figure 5, the production of TGF-β1 and IL-10 was low and IFN-γ was undetectable in normal mice. An increased production of TGF-β1 and IL-10 and a low production of IFN-γ was observed in the AC-injection group compared to the positive controls (p<0.001, p=0.032, and p=0.005, respectively). The production of these cytokines was not different between the positive controls and PBS controls. A higher production of TGF-β1 and IL-10 and a lower production of IFN-γ were found in the 72-h and 120-h group. However, a decreased production of TGF-β1 (both p<0.001) and IL-10 (p=0.009, p=0.013, respectively) and an increased production of IFN-γ (p=0.001, p=0.008, respectively) were detected in the 24-h and 48-h group compared to the AC-injection group.


A penetrating ocular injury can affect the induction of anterior chamber-associated immune deviation.

Lei F, Zhang J, Zhang J, He H, Du Y, Yang P - Mol. Vis. (2008)

Detection of TGF-β1 produced by splenocyte. Mice were sacrificed seven days after s.c. immunization. Spleens were removed and grinded to prepare a single-cell suspension. These cells were incubated in a complete RPMI 1640 medium with OVA (100 μg/ml) for 48 h in 24-well culture plates (2×106 cells/well). Supernatants were collected for determining the quantity of TGF-β1 by ELISA. Data are the mean ± SEM (n=6 per group). The asterisk indicates that p<0.05 as compared with the positive control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2255028&req=5

f3: Detection of TGF-β1 produced by splenocyte. Mice were sacrificed seven days after s.c. immunization. Spleens were removed and grinded to prepare a single-cell suspension. These cells were incubated in a complete RPMI 1640 medium with OVA (100 μg/ml) for 48 h in 24-well culture plates (2×106 cells/well). Supernatants were collected for determining the quantity of TGF-β1 by ELISA. Data are the mean ± SEM (n=6 per group). The asterisk indicates that p<0.05 as compared with the positive control.
Mentions: As cytokines, such as TGF-β1, IL-10, and IFN-γ, were involved in the induction of ACAID [21-25], our study examined whether there was an alteration in the production of these cytokines in the penetrating ocular injury groups. As shown in Figure 3, Figure 4, and Figure 5, the production of TGF-β1 and IL-10 was low and IFN-γ was undetectable in normal mice. An increased production of TGF-β1 and IL-10 and a low production of IFN-γ was observed in the AC-injection group compared to the positive controls (p<0.001, p=0.032, and p=0.005, respectively). The production of these cytokines was not different between the positive controls and PBS controls. A higher production of TGF-β1 and IL-10 and a lower production of IFN-γ were found in the 72-h and 120-h group. However, a decreased production of TGF-β1 (both p<0.001) and IL-10 (p=0.009, p=0.013, respectively) and an increased production of IFN-γ (p=0.001, p=0.008, respectively) were detected in the 24-h and 48-h group compared to the AC-injection group.

Bottom Line: However, the levels of IFN-gamma produced by splenocytes of the AC-injection group and the 72-h and 120-h group were significantly lower than those in the 24-h and 48-h group.An increased frequency of CD4(+)CD25(+)Foxp3(+)T cells was found in the AC-injection group and the 72-h and 120-h group.Penetrating ocular injury preformed shortly (24 h-48 h) after an AC injection of an antigen was able to abrogate ACAID and was associated with a decreased production of TGF-beta1 and IL-10, an increased production of IFN-gamma, and a decreased expression of CD4(+)CD25(+)Foxp3(+)T cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, First Affiliated Hospital, Department of Microbiology and Immunology, Zheng Zhou University, Zhengzhou, Henan Province, China.

ABSTRACT

Purpose: To determine the effect of penetrating ocular injury on the induction of anterior chamber-associated immune deviation (ACAID).

Methods: An injection of 5 mul ovalbumin (OVA, 20 mg/ml) into the anterior chamber (AC) of female BALB/c mice was performed to induce ACAID. A penetrating ocular injury was induced via the limbus on OVA-inoculated eyes at 24 h, 48 h, 72 h, and 120 h following AC injection. The mice receiving an OVA inoculation without the ocular injury served as the AC-injection group. Delayed type hypersensitivity (DTH) was examined to evaluate the induction of ACAID. The levels of transforming growth factor (TGF)-beta1, interleukin (IL)-10, and interferon (IFN)-gamma produced by splenocytes were detected by enzyme-linked immunosorbent assays (ELISA). The frequency of CD4(+)CD25(+)Foxp3(+)T cells in the splenocytes was detected by flow cytometry.

Results: A significantly decreased DTH response was observed in the AC-injection group as well as in mice that received a penetrating injury at 72 h and 120 h following AC-injection of OVA. The levels of TGF-beta1 and IL-10 produced by splenocytes of mice in the AC-injection group and in the 72-h and 120-h group were significant higher than those in the 24-h and 48-h group. However, the levels of IFN-gamma produced by splenocytes of the AC-injection group and the 72-h and 120-h group were significantly lower than those in the 24-h and 48-h group. An increased frequency of CD4(+)CD25(+)Foxp3(+)T cells was found in the AC-injection group and the 72-h and 120-h group.

Conclusions: Penetrating ocular injury preformed shortly (24 h-48 h) after an AC injection of an antigen was able to abrogate ACAID and was associated with a decreased production of TGF-beta1 and IL-10, an increased production of IFN-gamma, and a decreased expression of CD4(+)CD25(+)Foxp3(+)T cells.

Show MeSH
Related in: MedlinePlus