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Application of atomic force microscopy in morphological observation of antisense probe labeled with magnetism.

Wen M, Li B, Bai W, Li S, Yang X - Mol. Vis. (2008)

Bottom Line: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.Its morphology was observed with AFM.AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, The First Affiliated Hospital, College, Chongqing Medical University, Chongqing, China.

ABSTRACT

Purpose: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.

Methods: The c-erbB2 oncogene antisense probe labeled with SPIO was synthesized by a chemical cross-linking approach. Its morphology was observed with AFM.

Results: The chemical constitution of c-erbB2 oncogene antisense probes can be observed with AFM. The molecular structure of probes is easily visualized under AFM. Probes with diameters of 25-40 nm are in order, follow uniformity and the arrangement rule, can be separated from each other, and appear as cubes with a rugged surface morphology. Strong, low signals of the probes in transfected cells were observed by MR cellular imaging.

Conclusions: AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

Show MeSH
Magnetic resonance image of c-erbB2 antisense probe transfected SK-Br-3 cells. Transfected and untransfected cells, a probe culture medium, a no-probe culture medium, and distilled water were placed into Ependorf tubes and scanned using MR transactions. 1: untransfected cells, 2: no-probe culture medium, 3: transfected cells, 4: probe culture medium, 5: distilled water. From the figure, we can observe that c-erbB2 antisence probe can improve the magnetic properties of SK-Br-3 cells and decrease the signal intensity in MR scanning obviously.
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f3: Magnetic resonance image of c-erbB2 antisense probe transfected SK-Br-3 cells. Transfected and untransfected cells, a probe culture medium, a no-probe culture medium, and distilled water were placed into Ependorf tubes and scanned using MR transactions. 1: untransfected cells, 2: no-probe culture medium, 3: transfected cells, 4: probe culture medium, 5: distilled water. From the figure, we can observe that c-erbB2 antisence probe can improve the magnetic properties of SK-Br-3 cells and decrease the signal intensity in MR scanning obviously.

Mentions: To test the suitability of the c-erbB2 oncogene antisense probe for MR imaging, SK-Br-3 oncocytes were transfected with and without antisense probes. Transfected and untransfected cells, probe culture media, no-probe culture media, and distilled water were placed into Ependorf tubes and scanned by MR transactions. Untransfected cells, no-probe culture media, and distilled water distinctly showed a high signal. The group with distilled water had the highest signal, transfected cells and cells with probe culture media had distinctly low signals, and the transfected cells had the lowest signals (Figure 3). These results indicate that the c-erbB2 oncogene antisense probe is suitable for MR imaging.


Application of atomic force microscopy in morphological observation of antisense probe labeled with magnetism.

Wen M, Li B, Bai W, Li S, Yang X - Mol. Vis. (2008)

Magnetic resonance image of c-erbB2 antisense probe transfected SK-Br-3 cells. Transfected and untransfected cells, a probe culture medium, a no-probe culture medium, and distilled water were placed into Ependorf tubes and scanned using MR transactions. 1: untransfected cells, 2: no-probe culture medium, 3: transfected cells, 4: probe culture medium, 5: distilled water. From the figure, we can observe that c-erbB2 antisence probe can improve the magnetic properties of SK-Br-3 cells and decrease the signal intensity in MR scanning obviously.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2254963&req=5

f3: Magnetic resonance image of c-erbB2 antisense probe transfected SK-Br-3 cells. Transfected and untransfected cells, a probe culture medium, a no-probe culture medium, and distilled water were placed into Ependorf tubes and scanned using MR transactions. 1: untransfected cells, 2: no-probe culture medium, 3: transfected cells, 4: probe culture medium, 5: distilled water. From the figure, we can observe that c-erbB2 antisence probe can improve the magnetic properties of SK-Br-3 cells and decrease the signal intensity in MR scanning obviously.
Mentions: To test the suitability of the c-erbB2 oncogene antisense probe for MR imaging, SK-Br-3 oncocytes were transfected with and without antisense probes. Transfected and untransfected cells, probe culture media, no-probe culture media, and distilled water were placed into Ependorf tubes and scanned by MR transactions. Untransfected cells, no-probe culture media, and distilled water distinctly showed a high signal. The group with distilled water had the highest signal, transfected cells and cells with probe culture media had distinctly low signals, and the transfected cells had the lowest signals (Figure 3). These results indicate that the c-erbB2 oncogene antisense probe is suitable for MR imaging.

Bottom Line: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.Its morphology was observed with AFM.AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, The First Affiliated Hospital, College, Chongqing Medical University, Chongqing, China.

ABSTRACT

Purpose: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.

Methods: The c-erbB2 oncogene antisense probe labeled with SPIO was synthesized by a chemical cross-linking approach. Its morphology was observed with AFM.

Results: The chemical constitution of c-erbB2 oncogene antisense probes can be observed with AFM. The molecular structure of probes is easily visualized under AFM. Probes with diameters of 25-40 nm are in order, follow uniformity and the arrangement rule, can be separated from each other, and appear as cubes with a rugged surface morphology. Strong, low signals of the probes in transfected cells were observed by MR cellular imaging.

Conclusions: AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

Show MeSH