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Application of atomic force microscopy in morphological observation of antisense probe labeled with magnetism.

Wen M, Li B, Bai W, Li S, Yang X - Mol. Vis. (2008)

Bottom Line: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.Its morphology was observed with AFM.AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, The First Affiliated Hospital, College, Chongqing Medical University, Chongqing, China.

ABSTRACT

Purpose: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.

Methods: The c-erbB2 oncogene antisense probe labeled with SPIO was synthesized by a chemical cross-linking approach. Its morphology was observed with AFM.

Results: The chemical constitution of c-erbB2 oncogene antisense probes can be observed with AFM. The molecular structure of probes is easily visualized under AFM. Probes with diameters of 25-40 nm are in order, follow uniformity and the arrangement rule, can be separated from each other, and appear as cubes with a rugged surface morphology. Strong, low signals of the probes in transfected cells were observed by MR cellular imaging.

Conclusions: AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

Show MeSH
Elevation contour image of c-erbB2 oncogene antisense probe in the atomic force microscopy. The density of the c-erbB2 oncogene antisense probe was divided into 8 grades which are used to differentiate relative height, and the rugged surface morphology was marked with figures on image.
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f2: Elevation contour image of c-erbB2 oncogene antisense probe in the atomic force microscopy. The density of the c-erbB2 oncogene antisense probe was divided into 8 grades which are used to differentiate relative height, and the rugged surface morphology was marked with figures on image.

Mentions: The original image was treated with Gaussian filtering, speckle filtering, dust filtering, and scratch filtering, and after regulating brightness and contrast, a color image of the c-erbB2 oncogene antisense probe (Figure 1) and elevation contour image (Figure 2) were obtained. Graduations on coordinate axes in all images are pixel values, and indication ranges of the X and Y directions are 350 nm with a total of 1000 pixel levels. Reds, greens, and blues in the color images reflected probe density. Red represented the highest density, green the second highest, and blue the lowest (Figure 1A). Based on the atom and basic groups observed with AFM, the structural relationship between dextran and ASODN is clearly visualized (Figure 1B). Dextran loops, carbochain linking amino group and six carbon atoms of ASODN, and deoxyribose loops were observed. Thus, ASODN is linked closely with dextran and are arranged in an orderly manner. The total diameter of the antisense probe was 25–40 nm, and the length of ASODN is 5 nm. In the elevation contour image, the density of the sample was divided into eight grades, which are used to differentiate relative height; the rugged surface morphology was marked with figures on the image.


Application of atomic force microscopy in morphological observation of antisense probe labeled with magnetism.

Wen M, Li B, Bai W, Li S, Yang X - Mol. Vis. (2008)

Elevation contour image of c-erbB2 oncogene antisense probe in the atomic force microscopy. The density of the c-erbB2 oncogene antisense probe was divided into 8 grades which are used to differentiate relative height, and the rugged surface morphology was marked with figures on image.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2254963&req=5

f2: Elevation contour image of c-erbB2 oncogene antisense probe in the atomic force microscopy. The density of the c-erbB2 oncogene antisense probe was divided into 8 grades which are used to differentiate relative height, and the rugged surface morphology was marked with figures on image.
Mentions: The original image was treated with Gaussian filtering, speckle filtering, dust filtering, and scratch filtering, and after regulating brightness and contrast, a color image of the c-erbB2 oncogene antisense probe (Figure 1) and elevation contour image (Figure 2) were obtained. Graduations on coordinate axes in all images are pixel values, and indication ranges of the X and Y directions are 350 nm with a total of 1000 pixel levels. Reds, greens, and blues in the color images reflected probe density. Red represented the highest density, green the second highest, and blue the lowest (Figure 1A). Based on the atom and basic groups observed with AFM, the structural relationship between dextran and ASODN is clearly visualized (Figure 1B). Dextran loops, carbochain linking amino group and six carbon atoms of ASODN, and deoxyribose loops were observed. Thus, ASODN is linked closely with dextran and are arranged in an orderly manner. The total diameter of the antisense probe was 25–40 nm, and the length of ASODN is 5 nm. In the elevation contour image, the density of the sample was divided into eight grades, which are used to differentiate relative height; the rugged surface morphology was marked with figures on the image.

Bottom Line: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.Its morphology was observed with AFM.AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, The First Affiliated Hospital, College, Chongqing Medical University, Chongqing, China.

ABSTRACT

Purpose: To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.

Methods: The c-erbB2 oncogene antisense probe labeled with SPIO was synthesized by a chemical cross-linking approach. Its morphology was observed with AFM.

Results: The chemical constitution of c-erbB2 oncogene antisense probes can be observed with AFM. The molecular structure of probes is easily visualized under AFM. Probes with diameters of 25-40 nm are in order, follow uniformity and the arrangement rule, can be separated from each other, and appear as cubes with a rugged surface morphology. Strong, low signals of the probes in transfected cells were observed by MR cellular imaging.

Conclusions: AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.

Show MeSH