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Defining junctional complexes

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Several investigators singled out the spines of certain epidermal cells as contact points and potential intercellular channels... Bizzozero confirmed that these densely staining regions were contact points but found that the cytoplasm was not continuous between the cells... Next was the zonula adherens (adhering belt; now known as the adherens junction) with an intercellular space separating strictly parallel membranes, all framed by dense cytoplasmic matrices... Most basal were the desmosomes (here named the macula adhaerens [adhering spot], a name that did not last)... But in response the epithelium formed intercellular connections that were “tightened and increased in depth” (Farquhar and Palade, 1961)... That morphological change associated with increased permeability made Marilyn Farquhar take a closer look... You have to have a reason for looking, and people hadn't had that functional connection before. ” The new work surveyed junctions in 13 different epithelial tissues in exhaustive detail... But it was just as demanding as it is now to put that structure into a functional context. ” Many people had excellent microscopes and high quality preparations, but “once it got past the looking they were stuck. ” Adding the functional data, such as the impermeability of tight junctions to protein tracers, “was really a hallmark of the Rockefeller school at that time,” she says... The initial study reported on the orderly organization of a columnar epithelium, but a similar hierarchy was found in the less rigidly organized stratified cells of the epidermis (specifically in the basal layer; Farquhar and Palade, 1965)... The depth (number of occluding strands) in a tight junction was later found to correlate with the leakiness or tightness of a tight junction (Claude and Goodenough, 1973)... Eventually, the molecular components of the junctional complexes were determined: the occludin (Furuse et al., 1993) and claudins (Furuse et al., 1998) of tight junctions; actin-linked cadherins of adherens junctions; and intermediate filament-linked cadherins of desmosomes.

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Three in one: The EM of an intestinal epithelium reveals a tight junction (arrows 1 to 2), adherens junction (arrows 2 to 3), and desmosomes (arrows 4 to 5).FARQUHAR
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uro1: Three in one: The EM of an intestinal epithelium reveals a tight junction (arrows 1 to 2), adherens junction (arrows 2 to 3), and desmosomes (arrows 4 to 5).FARQUHAR


Defining junctional complexes
Three in one: The EM of an intestinal epithelium reveals a tight junction (arrows 1 to 2), adherens junction (arrows 2 to 3), and desmosomes (arrows 4 to 5).FARQUHAR
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2254751&req=5

uro1: Three in one: The EM of an intestinal epithelium reveals a tight junction (arrows 1 to 2), adherens junction (arrows 2 to 3), and desmosomes (arrows 4 to 5).FARQUHAR

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Several investigators singled out the spines of certain epidermal cells as contact points and potential intercellular channels... Bizzozero confirmed that these densely staining regions were contact points but found that the cytoplasm was not continuous between the cells... Next was the zonula adherens (adhering belt; now known as the adherens junction) with an intercellular space separating strictly parallel membranes, all framed by dense cytoplasmic matrices... Most basal were the desmosomes (here named the macula adhaerens [adhering spot], a name that did not last)... But in response the epithelium formed intercellular connections that were “tightened and increased in depth” (Farquhar and Palade, 1961)... That morphological change associated with increased permeability made Marilyn Farquhar take a closer look... You have to have a reason for looking, and people hadn't had that functional connection before. ” The new work surveyed junctions in 13 different epithelial tissues in exhaustive detail... But it was just as demanding as it is now to put that structure into a functional context. ” Many people had excellent microscopes and high quality preparations, but “once it got past the looking they were stuck. ” Adding the functional data, such as the impermeability of tight junctions to protein tracers, “was really a hallmark of the Rockefeller school at that time,” she says... The initial study reported on the orderly organization of a columnar epithelium, but a similar hierarchy was found in the less rigidly organized stratified cells of the epidermis (specifically in the basal layer; Farquhar and Palade, 1965)... The depth (number of occluding strands) in a tight junction was later found to correlate with the leakiness or tightness of a tight junction (Claude and Goodenough, 1973)... Eventually, the molecular components of the junctional complexes were determined: the occludin (Furuse et al., 1993) and claudins (Furuse et al., 1998) of tight junctions; actin-linked cadherins of adherens junctions; and intermediate filament-linked cadherins of desmosomes.

No MeSH data available.