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Peptide mimicrying between SARS coronavirus spike protein and human proteins reacts with SARS patient serum.

Hwa KY, Lin WM, Hou YI, Yeh TM - J. Biomed. Biotechnol. (2008)

Bottom Line: In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins.Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8.The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Engineering, Center for Biomedical Industries, National Taipei University of Technology, Taipei 106, Taiwan.

ABSTRACT
Molecular mimicry, defined as similar structures shared by molecules from dissimilar genes or proteins, is a general strategy used by pathogens to infect host cells. Severe acute respiratory syndrome (SARS) is a new human respiratory infectious disease caused by SARS coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV plays an important role in the virus entry into a cell. In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins. Two of the peptides D07 (residues 927-937) and D08 (residues 942-951) were recognized by the sera of SARS patients. Murine hyperimmune sera against these peptides bound to proteins of human lung epithelial cells A549. Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8. The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

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IL-8 production of A549 cells induced by D10 peptide and AngI. A549 cells () were incubated with or without peptides for48 hours. The levels of IL-8 in the culture supernatants were assayed as described in Section 2. Data represents themean ± SD of triplicates.
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fig8: IL-8 production of A549 cells induced by D10 peptide and AngI. A549 cells () were incubated with or without peptides for48 hours. The levels of IL-8 in the culture supernatants were assayed as described in Section 2. Data represents themean ± SD of triplicates.

Mentions: To understand whether D10 has similar biological activity as Ang I, we incubatedVero cells and lung epithelial A549 cells with D10, Ang 1, or control peptide D11. Both Vero and A549 cells were induced to proliferate in the presence of D10 andAng I but not the control peptide (see Figure 7). In addition, D10 and Ang I couldalso induce chemokine IL-8 production of A549 cells (see Figure 8).


Peptide mimicrying between SARS coronavirus spike protein and human proteins reacts with SARS patient serum.

Hwa KY, Lin WM, Hou YI, Yeh TM - J. Biomed. Biotechnol. (2008)

IL-8 production of A549 cells induced by D10 peptide and AngI. A549 cells () were incubated with or without peptides for48 hours. The levels of IL-8 in the culture supernatants were assayed as described in Section 2. Data represents themean ± SD of triplicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2254527&req=5

fig8: IL-8 production of A549 cells induced by D10 peptide and AngI. A549 cells () were incubated with or without peptides for48 hours. The levels of IL-8 in the culture supernatants were assayed as described in Section 2. Data represents themean ± SD of triplicates.
Mentions: To understand whether D10 has similar biological activity as Ang I, we incubatedVero cells and lung epithelial A549 cells with D10, Ang 1, or control peptide D11. Both Vero and A549 cells were induced to proliferate in the presence of D10 andAng I but not the control peptide (see Figure 7). In addition, D10 and Ang I couldalso induce chemokine IL-8 production of A549 cells (see Figure 8).

Bottom Line: In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins.Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8.The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Engineering, Center for Biomedical Industries, National Taipei University of Technology, Taipei 106, Taiwan.

ABSTRACT
Molecular mimicry, defined as similar structures shared by molecules from dissimilar genes or proteins, is a general strategy used by pathogens to infect host cells. Severe acute respiratory syndrome (SARS) is a new human respiratory infectious disease caused by SARS coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV plays an important role in the virus entry into a cell. In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins. Two of the peptides D07 (residues 927-937) and D08 (residues 942-951) were recognized by the sera of SARS patients. Murine hyperimmune sera against these peptides bound to proteins of human lung epithelial cells A549. Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8. The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

Show MeSH
Related in: MedlinePlus