Limits...
Peptide mimicrying between SARS coronavirus spike protein and human proteins reacts with SARS patient serum.

Hwa KY, Lin WM, Hou YI, Yeh TM - J. Biomed. Biotechnol. (2008)

Bottom Line: In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins.Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8.The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Engineering, Center for Biomedical Industries, National Taipei University of Technology, Taipei 106, Taiwan.

ABSTRACT
Molecular mimicry, defined as similar structures shared by molecules from dissimilar genes or proteins, is a general strategy used by pathogens to infect host cells. Severe acute respiratory syndrome (SARS) is a new human respiratory infectious disease caused by SARS coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV plays an important role in the virus entry into a cell. In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins. Two of the peptides D07 (residues 927-937) and D08 (residues 942-951) were recognized by the sera of SARS patients. Murine hyperimmune sera against these peptides bound to proteins of human lung epithelial cells A549. Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8. The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

Show MeSH

Related in: MedlinePlus

Mouse hyperimmune sera against D08 peptide recognizeproteins in the A549 cell lysate. Proteins in the cell lysate of A549 cellswere separated by SDS-PAGE and transferred to membrane as described in Section 2.Western blots against this membrane using normal mouse sera (a) or hyperimmunesera against D08 (b) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2254527&req=5

fig5: Mouse hyperimmune sera against D08 peptide recognizeproteins in the A549 cell lysate. Proteins in the cell lysate of A549 cellswere separated by SDS-PAGE and transferred to membrane as described in Section 2.Western blots against this membrane using normal mouse sera (a) or hyperimmunesera against D08 (b) are shown.

Mentions: To test whether synthetic peptides D01, D07, and D08 can induce antibodies crossreacted withhuman proteins, we immunized mice with these peptides to generate hyperimmunesera against these peptides. We found hyperimmune sera against D08 can bind tothe cytoplasmic region of human A549 cells as demonstrated by immunofluorescentstain (see Figure 4). Using Western blot analysis, hyperimmune sera against D08could recognize more bands in A549 cell lysate as compared to normal mice sera(see Figure 5). In addition, hyperimmune sera against D07, but not D01, showedsimilar crossreactivity to A549 cells as hyperimmune sera against D08 did (datanot shown).


Peptide mimicrying between SARS coronavirus spike protein and human proteins reacts with SARS patient serum.

Hwa KY, Lin WM, Hou YI, Yeh TM - J. Biomed. Biotechnol. (2008)

Mouse hyperimmune sera against D08 peptide recognizeproteins in the A549 cell lysate. Proteins in the cell lysate of A549 cellswere separated by SDS-PAGE and transferred to membrane as described in Section 2.Western blots against this membrane using normal mouse sera (a) or hyperimmunesera against D08 (b) are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2254527&req=5

fig5: Mouse hyperimmune sera against D08 peptide recognizeproteins in the A549 cell lysate. Proteins in the cell lysate of A549 cellswere separated by SDS-PAGE and transferred to membrane as described in Section 2.Western blots against this membrane using normal mouse sera (a) or hyperimmunesera against D08 (b) are shown.
Mentions: To test whether synthetic peptides D01, D07, and D08 can induce antibodies crossreacted withhuman proteins, we immunized mice with these peptides to generate hyperimmunesera against these peptides. We found hyperimmune sera against D08 can bind tothe cytoplasmic region of human A549 cells as demonstrated by immunofluorescentstain (see Figure 4). Using Western blot analysis, hyperimmune sera against D08could recognize more bands in A549 cell lysate as compared to normal mice sera(see Figure 5). In addition, hyperimmune sera against D07, but not D01, showedsimilar crossreactivity to A549 cells as hyperimmune sera against D08 did (datanot shown).

Bottom Line: In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins.Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8.The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Science and Engineering, Center for Biomedical Industries, National Taipei University of Technology, Taipei 106, Taiwan.

ABSTRACT
Molecular mimicry, defined as similar structures shared by molecules from dissimilar genes or proteins, is a general strategy used by pathogens to infect host cells. Severe acute respiratory syndrome (SARS) is a new human respiratory infectious disease caused by SARS coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV plays an important role in the virus entry into a cell. In this study, eleven synthetic peptides from the S protein were selected based on its sequence homology with human proteins. Two of the peptides D07 (residues 927-937) and D08 (residues 942-951) were recognized by the sera of SARS patients. Murine hyperimmune sera against these peptides bound to proteins of human lung epithelial cells A549. Another peptide D10 (residues 490-502) stimulated A549 to proliferate and secrete IL-8. The present results suggest that the selected S protein regions, which share sequence homology with human proteins, may play important roles in SARS-CoV infection.

Show MeSH
Related in: MedlinePlus