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Superparamagnetic iron oxide nanoparticles coated with galactose-carrying polymer for hepatocyte targeting.

Yoo MK, Kim IY, Kim EM, Jeong HJ, Lee CM, Jeong YY, Akaike T, Cho CS - J. Biomed. Biotechnol. (2007)

Bottom Line: Our goal is to develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating the capacities to be delivered in liver specifically and to be dispersed in physiological environment stably.For this purpose, SPIONs were coated with polyvinylbenzyl-O-beta-D-galactopyranosyl-D-gluconamide (PVLA) having galactose moieties to be recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes.The sizes, size distribution, structure, and coating of the nanoparticles were characterized by transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), and Fourier transform infrared (FT-IR), respectively.

View Article: PubMed Central - PubMed

Affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.

ABSTRACT
Our goal is to develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating the capacities to be delivered in liver specifically and to be dispersed in physiological environment stably. For this purpose, SPIONs were coated with polyvinylbenzyl-O-beta-D-galactopyranosyl-D-gluconamide (PVLA) having galactose moieties to be recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes. For use as a control, we also prepared SPIONs coordinated with 2-pyrrolidone. The sizes, size distribution, structure, and coating of the nanoparticles were characterized by transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), and Fourier transform infrared (FT-IR), respectively. Intracellular uptake of the PVLA-coated SPIONs was visualized by confocal laser scanning microscopy, and their hepatocyte-specific delivery was also investigated through magnetic resonance (MR) images of rat liver. MRI experimental results indicated that the PVLA-coated SPIONs possess the more specific accumulation property in liver compared with control, which suggests their potential utility as liver-targeting MRI contrast agent.

No MeSH data available.


Related in: MedlinePlus

Chemical structure of PVLA, β-galactose-carrying polymer.
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fig1: Chemical structure of PVLA, β-galactose-carrying polymer.

Mentions: Ferric chloride hexahydrate (FeCl3·6H2O > 97%) and ferrous chloride tetrahydrate (FeCl2·4H2O > 99%) were purchased from Sigma-Aldrich (St.Luois, Miss, USA). PVLA (MW = )was prepared by the same method previously reported [23]. The chemicalstructure of PVLA is shown in Figure 1. Fluorescencelabeling of PVLA was performed similar to the method previously described [22].All other chemicals were of analytical reagent grade and were used without furtherpurification.


Superparamagnetic iron oxide nanoparticles coated with galactose-carrying polymer for hepatocyte targeting.

Yoo MK, Kim IY, Kim EM, Jeong HJ, Lee CM, Jeong YY, Akaike T, Cho CS - J. Biomed. Biotechnol. (2007)

Chemical structure of PVLA, β-galactose-carrying polymer.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2254526&req=5

fig1: Chemical structure of PVLA, β-galactose-carrying polymer.
Mentions: Ferric chloride hexahydrate (FeCl3·6H2O > 97%) and ferrous chloride tetrahydrate (FeCl2·4H2O > 99%) were purchased from Sigma-Aldrich (St.Luois, Miss, USA). PVLA (MW = )was prepared by the same method previously reported [23]. The chemicalstructure of PVLA is shown in Figure 1. Fluorescencelabeling of PVLA was performed similar to the method previously described [22].All other chemicals were of analytical reagent grade and were used without furtherpurification.

Bottom Line: Our goal is to develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating the capacities to be delivered in liver specifically and to be dispersed in physiological environment stably.For this purpose, SPIONs were coated with polyvinylbenzyl-O-beta-D-galactopyranosyl-D-gluconamide (PVLA) having galactose moieties to be recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes.The sizes, size distribution, structure, and coating of the nanoparticles were characterized by transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), and Fourier transform infrared (FT-IR), respectively.

View Article: PubMed Central - PubMed

Affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.

ABSTRACT
Our goal is to develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating the capacities to be delivered in liver specifically and to be dispersed in physiological environment stably. For this purpose, SPIONs were coated with polyvinylbenzyl-O-beta-D-galactopyranosyl-D-gluconamide (PVLA) having galactose moieties to be recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes. For use as a control, we also prepared SPIONs coordinated with 2-pyrrolidone. The sizes, size distribution, structure, and coating of the nanoparticles were characterized by transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), and Fourier transform infrared (FT-IR), respectively. Intracellular uptake of the PVLA-coated SPIONs was visualized by confocal laser scanning microscopy, and their hepatocyte-specific delivery was also investigated through magnetic resonance (MR) images of rat liver. MRI experimental results indicated that the PVLA-coated SPIONs possess the more specific accumulation property in liver compared with control, which suggests their potential utility as liver-targeting MRI contrast agent.

No MeSH data available.


Related in: MedlinePlus