Limits...
Flipping the fusion switch

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Calcium and calmodulin transfer a critical region of the vesicle protein VAMP from donor to target membrane, suggest Luc de Haro, Michael Seagar (INSERM, Marseille, France), and colleagues... VAMP is a v-SNARE: a helical protein sticking out of donor vesicles that zippers up with t-SNAREs on target membranes... Ca/calmodulin binds to VAMP-loaded vesicles, and is needed for target membranes to bind to the VAMP on VAMP-loaded vesicles... But how important is this for fusion? Ca/calmodulin was an early favorite of those looking for a fusion trigger but later fell out of favor when it became clear that inhibitors had pleiotropic effects on numerous kinases and channels... But Seagar points out that several groups have now observed the inhibitory property of the source membrane on SNARE activity... The new results show how Ca/calmodulin can overcome this inhibition and perhaps convert it into fusion... Ca/calmodulin is not the whole story... Seagar suggests that it may be an archaic fusion trigger used in yeast and some intracellular trafficking events in mammals... But in the nervous system, where a faster trigger was needed, it may have been superseded by synaptotagmin. ▪ Reference:

No MeSH data available.


Calmodulin binding (left) displaces part of VAMP from cis membranes, but trans membranes then displace calmodulin (right).Seagar/NAS
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2249915&req=5

uro1: Calmodulin binding (left) displaces part of VAMP from cis membranes, but trans membranes then displace calmodulin (right).Seagar/NAS


Flipping the fusion switch
Calmodulin binding (left) displaces part of VAMP from cis membranes, but trans membranes then displace calmodulin (right).Seagar/NAS
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2249915&req=5

uro1: Calmodulin binding (left) displaces part of VAMP from cis membranes, but trans membranes then displace calmodulin (right).Seagar/NAS

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Calcium and calmodulin transfer a critical region of the vesicle protein VAMP from donor to target membrane, suggest Luc de Haro, Michael Seagar (INSERM, Marseille, France), and colleagues... VAMP is a v-SNARE: a helical protein sticking out of donor vesicles that zippers up with t-SNAREs on target membranes... Ca/calmodulin binds to VAMP-loaded vesicles, and is needed for target membranes to bind to the VAMP on VAMP-loaded vesicles... But how important is this for fusion? Ca/calmodulin was an early favorite of those looking for a fusion trigger but later fell out of favor when it became clear that inhibitors had pleiotropic effects on numerous kinases and channels... But Seagar points out that several groups have now observed the inhibitory property of the source membrane on SNARE activity... The new results show how Ca/calmodulin can overcome this inhibition and perhaps convert it into fusion... Ca/calmodulin is not the whole story... Seagar suggests that it may be an archaic fusion trigger used in yeast and some intracellular trafficking events in mammals... But in the nervous system, where a faster trigger was needed, it may have been superseded by synaptotagmin. ▪ Reference:

No MeSH data available.