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Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum sanctum Linn in Rats.

Shetty S, Udupa S, Udupa L - Evid Based Complement Alternat Med (2008)

Bottom Line: Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum.Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group.The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Melaka Manipal Medical College, Department of Biochemistry, KMC International Centre and Department of Pharmacology, Kasturba Medical College, Manipal 576104, India.

ABSTRACT
In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Ocimum sanctum (O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

No MeSH data available.


Related in: MedlinePlus

Effect of alcoholic and aqueous extracts of O. sanctum on antioxidant parameters. (A) SOD activity in granulation tissue; (B) catalase activity in granulation tissue; (C) reduced GSH level in granulation tissue; (D)lipid peroxidation-MDA level in granulation tissue.
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Figure 3: Effect of alcoholic and aqueous extracts of O. sanctum on antioxidant parameters. (A) SOD activity in granulation tissue; (B) catalase activity in granulation tissue; (C) reduced GSH level in granulation tissue; (D)lipid peroxidation-MDA level in granulation tissue.

Mentions: SOD activity in granulation tissue was significantly increased in the case of rats treated with alcoholic and aqueous extract 400 mg/kg (P < 0.05), alcoholic 800 mg/kg (P < 0.05) and aqueous extract 800 mg/kg (P < 0.01) when compared with control (Fig. 3A). Catalase level in granulation tissue was significantly increased in the case of alcoholic and aqueous extract 400 mg/kg (P < 0.05), alcoholic 800 mg/kg (P < 0.05) and aqueous extract 800 mg/kg treated groups (P < 0.01), when compared with control (Fig. 3B). Reduced GSH concentration in granulation tissue was significantly increased in rats treated with different extracts (P < 0.001), when compared with control. There was significant increase in GSH level in aqueous 800 mg/kg treated group when compared with alcoholic 400 mg/kg group (P < 0.05) in intercomparison statistical analysis (Fig. 3C). Malondialdehyde (MDA) level (which indicates lipid peroxidation) in granulation tissue was significantly decreased in the case of all the extract-treatment groups (P < 0.001) compared with controls (Fig. 3D).Figure 3.


Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum sanctum Linn in Rats.

Shetty S, Udupa S, Udupa L - Evid Based Complement Alternat Med (2008)

Effect of alcoholic and aqueous extracts of O. sanctum on antioxidant parameters. (A) SOD activity in granulation tissue; (B) catalase activity in granulation tissue; (C) reduced GSH level in granulation tissue; (D)lipid peroxidation-MDA level in granulation tissue.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2249741&req=5

Figure 3: Effect of alcoholic and aqueous extracts of O. sanctum on antioxidant parameters. (A) SOD activity in granulation tissue; (B) catalase activity in granulation tissue; (C) reduced GSH level in granulation tissue; (D)lipid peroxidation-MDA level in granulation tissue.
Mentions: SOD activity in granulation tissue was significantly increased in the case of rats treated with alcoholic and aqueous extract 400 mg/kg (P < 0.05), alcoholic 800 mg/kg (P < 0.05) and aqueous extract 800 mg/kg (P < 0.01) when compared with control (Fig. 3A). Catalase level in granulation tissue was significantly increased in the case of alcoholic and aqueous extract 400 mg/kg (P < 0.05), alcoholic 800 mg/kg (P < 0.05) and aqueous extract 800 mg/kg treated groups (P < 0.01), when compared with control (Fig. 3B). Reduced GSH concentration in granulation tissue was significantly increased in rats treated with different extracts (P < 0.001), when compared with control. There was significant increase in GSH level in aqueous 800 mg/kg treated group when compared with alcoholic 400 mg/kg group (P < 0.05) in intercomparison statistical analysis (Fig. 3C). Malondialdehyde (MDA) level (which indicates lipid peroxidation) in granulation tissue was significantly decreased in the case of all the extract-treatment groups (P < 0.001) compared with controls (Fig. 3D).Figure 3.

Bottom Line: Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum.Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group.The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Melaka Manipal Medical College, Department of Biochemistry, KMC International Centre and Department of Pharmacology, Kasturba Medical College, Manipal 576104, India.

ABSTRACT
In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Ocimum sanctum (O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

No MeSH data available.


Related in: MedlinePlus