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Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum sanctum Linn in Rats.

Shetty S, Udupa S, Udupa L - Evid Based Complement Alternat Med (2008)

Bottom Line: Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum.Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group.The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Melaka Manipal Medical College, Department of Biochemistry, KMC International Centre and Department of Pharmacology, Kasturba Medical College, Manipal 576104, India.

ABSTRACT
In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Ocimum sanctum (O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

No MeSH data available.


Related in: MedlinePlus

Effect of aqueous and alcoholic extracts of O. sanctum on excision wound model.
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Figure 2: Effect of aqueous and alcoholic extracts of O. sanctum on excision wound model.

Mentions: In the excision wound model, the percent of wound contraction was measured on alternate days. The percentage wound contraction was nearly 100% for the both the doses of alcoholic extract in 16 days while for the control it was 22 days (P < 0.05). The percentage wound contraction was nearly 100% for the both the doses of aqueous extract in 14 days in comparison with controls (P < 0.05) (Fig. 2). In the above physiological and pharmacological parameters of wound healing, aqueous extract of O. sanctum was more effective than the alcoholic extract.Figure 2.


Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum sanctum Linn in Rats.

Shetty S, Udupa S, Udupa L - Evid Based Complement Alternat Med (2008)

Effect of aqueous and alcoholic extracts of O. sanctum on excision wound model.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2249741&req=5

Figure 2: Effect of aqueous and alcoholic extracts of O. sanctum on excision wound model.
Mentions: In the excision wound model, the percent of wound contraction was measured on alternate days. The percentage wound contraction was nearly 100% for the both the doses of alcoholic extract in 16 days while for the control it was 22 days (P < 0.05). The percentage wound contraction was nearly 100% for the both the doses of aqueous extract in 14 days in comparison with controls (P < 0.05) (Fig. 2). In the above physiological and pharmacological parameters of wound healing, aqueous extract of O. sanctum was more effective than the alcoholic extract.Figure 2.

Bottom Line: Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum.Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group.The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Melaka Manipal Medical College, Department of Biochemistry, KMC International Centre and Department of Pharmacology, Kasturba Medical College, Manipal 576104, India.

ABSTRACT
In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Ocimum sanctum (O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract of O. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest that O. sanctum has antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

No MeSH data available.


Related in: MedlinePlus