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Phellinus linteus Extract Augments the Immune Response in Mitomycin C-Induced Immunodeficient Mice.

Matsuba S, Matsuno H, Sakuma M, Komatsu Y - Evid Based Complement Alternat Med (2008)

Bottom Line: MMC groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration.The number of PFC per 10(6) spleen cells increased significantly in the PLW (2 g/kg/day) group when compared with the MMC-control (P < 0.05) while populations of IFNgamma- and IL-4-producing T lymphocytes decreased by MMC treatment.Our results indicated that PLW augments the immune response of the spleen in MMC-induced immunodeficient mice.

View Article: PubMed Central - PubMed

Affiliation: Kanazawa Medical University, Department of Fundamental Research for CAM, Uchinada, Ishikawa prefecture and Sun R&D Institute for Natural Medicines Co, Inc., Tokyo, Japan.

ABSTRACT
Phellinus linteus is a fungus distributed throughout Japan, Korea and China. Boiled water-soluble extracts from P. linteus (PLW) have shown anti-tumor and immunomodulatory properties in experiments done by intraperitoneal treatment, or in in vitro cell cultures. This is the first investigation on how oral administration of PLW influences immune responses. Here, we established immunodeficient mice by mitomycin C (MMC) and then researched how PLW influenced plaque-forming cell (PFC) production and populations of cytokine [interferon- (IFNgamma-) and interleukin-4 (IL-4)]-producing T lymphocytes. PLW samples were administered orally for 19 days (1, 2 or 4 g/kg/day). PFC assay was followed using Jerne's method. IFN- and IL-4-producing T lymphocyte populations were measured by flow-activated cell sorter (FACS). These assays were conducted the day after the last oral administration. MMC groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration. The number of PFC per 10(6) spleen cells increased significantly in the PLW (2 g/kg/day) group when compared with the MMC-control (P < 0.05) while populations of IFNgamma- and IL-4-producing T lymphocytes decreased by MMC treatment. However, the PLW group tended to increase more than the MMC-control. Our results indicated that PLW augments the immune response of the spleen in MMC-induced immunodeficient mice.

No MeSH data available.


Related in: MedlinePlus

The experimental schedule of PLW on immune response.
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Figure 1: The experimental schedule of PLW on immune response.

Mentions: The schedule is shown in Fig. 1. Mice were divided into five groups (Control, MMC-control, MMC-PLW1, MMC-PLW2 and MMC-PLW3). Control and MMC-control groups were given distilled water, and MMC-PLW groups were administered orally 1, 2 or 4 g/kg/day of PLW for 19 days. MMC-groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration (28–30). The day after the last administration, we measured weight change in the spleen, researched PFC production and the population of cytokine [interferon-γ (IFNγ) and interleukin-4 (IL-4)]-producing cells in the spleen.Figure 1.


Phellinus linteus Extract Augments the Immune Response in Mitomycin C-Induced Immunodeficient Mice.

Matsuba S, Matsuno H, Sakuma M, Komatsu Y - Evid Based Complement Alternat Med (2008)

The experimental schedule of PLW on immune response.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2249738&req=5

Figure 1: The experimental schedule of PLW on immune response.
Mentions: The schedule is shown in Fig. 1. Mice were divided into five groups (Control, MMC-control, MMC-PLW1, MMC-PLW2 and MMC-PLW3). Control and MMC-control groups were given distilled water, and MMC-PLW groups were administered orally 1, 2 or 4 g/kg/day of PLW for 19 days. MMC-groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration (28–30). The day after the last administration, we measured weight change in the spleen, researched PFC production and the population of cytokine [interferon-γ (IFNγ) and interleukin-4 (IL-4)]-producing cells in the spleen.Figure 1.

Bottom Line: MMC groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration.The number of PFC per 10(6) spleen cells increased significantly in the PLW (2 g/kg/day) group when compared with the MMC-control (P < 0.05) while populations of IFNgamma- and IL-4-producing T lymphocytes decreased by MMC treatment.Our results indicated that PLW augments the immune response of the spleen in MMC-induced immunodeficient mice.

View Article: PubMed Central - PubMed

Affiliation: Kanazawa Medical University, Department of Fundamental Research for CAM, Uchinada, Ishikawa prefecture and Sun R&D Institute for Natural Medicines Co, Inc., Tokyo, Japan.

ABSTRACT
Phellinus linteus is a fungus distributed throughout Japan, Korea and China. Boiled water-soluble extracts from P. linteus (PLW) have shown anti-tumor and immunomodulatory properties in experiments done by intraperitoneal treatment, or in in vitro cell cultures. This is the first investigation on how oral administration of PLW influences immune responses. Here, we established immunodeficient mice by mitomycin C (MMC) and then researched how PLW influenced plaque-forming cell (PFC) production and populations of cytokine [interferon- (IFNgamma-) and interleukin-4 (IL-4)]-producing T lymphocytes. PLW samples were administered orally for 19 days (1, 2 or 4 g/kg/day). PFC assay was followed using Jerne's method. IFN- and IL-4-producing T lymphocyte populations were measured by flow-activated cell sorter (FACS). These assays were conducted the day after the last oral administration. MMC groups were given MMC (1 mg/kg/day) intraperitoneally for 6 days with PLW administration. The number of PFC per 10(6) spleen cells increased significantly in the PLW (2 g/kg/day) group when compared with the MMC-control (P < 0.05) while populations of IFNgamma- and IL-4-producing T lymphocytes decreased by MMC treatment. However, the PLW group tended to increase more than the MMC-control. Our results indicated that PLW augments the immune response of the spleen in MMC-induced immunodeficient mice.

No MeSH data available.


Related in: MedlinePlus