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Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.

Secor ER, Carson WF, Singh A, Pensa M, Guernsey LA, Schramm CM, Thrall RS - Evid Based Complement Alternat Med (2008)

Bottom Line: Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models.Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P </= 0.01), reduction in BAL eosinophils (P </= 0.02) and IL-13 concentrations (P </= 0.04) as compared with PBS controls.These results suggest that oral treatment with bromelain had a beneficial therapeutic effect in this murine model of asthma and bromelain may also be effective in human conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology and Department of Pediatrics, University of Connecticut School of Medicine, Farmington, CT, USA.

ABSTRACT
Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). To establish AAD, female C57BL/6J mice were sensitized with intraperitoneal (i.p.) OVA/alum and then challenged with OVA aerosols for 3 days. Mice were gavaged with either (phosphate buffered saline)PBS or 200 mg/kg bromelain in PBS, twice daily for four consecutive days, beginning 1 day prior to OVA aerosol challenge. Airway reactivity and methacholine sensitivity, bronchoalveolar lavage (BAL) cellular differential, Th2 cytokines IL-5 and IL-13, and lung histology were compared between treatment groups. Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P

No MeSH data available.


Related in: MedlinePlus

The effect of oral Bromelain treatment on airway reactivity and methacholine sensitivity. At baseline (open circles), Bromelain-treated and saline-treated mice had similar reactivity (P = 0.50) and sensitivity to methacholine (p2 values 48 ± 13 mg/ml in Bromelain treated versus 64 ± 15 mg/ml in saline treated; P = 0.47). After 3 OVA aerosol exposures (filled circles), saline treated mice tended to be more reactive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B) whereas no change occurred in Bromelain treated mice in reactivity (P = 0.52; A) and sensitivity (P = 0.45; B). Comparisons were made by paired t-test and repeated measures ANOVA; n = 7–8 animals per group.
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Figure 5: The effect of oral Bromelain treatment on airway reactivity and methacholine sensitivity. At baseline (open circles), Bromelain-treated and saline-treated mice had similar reactivity (P = 0.50) and sensitivity to methacholine (p2 values 48 ± 13 mg/ml in Bromelain treated versus 64 ± 15 mg/ml in saline treated; P = 0.47). After 3 OVA aerosol exposures (filled circles), saline treated mice tended to be more reactive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B) whereas no change occurred in Bromelain treated mice in reactivity (P = 0.52; A) and sensitivity (P = 0.45; B). Comparisons were made by paired t-test and repeated measures ANOVA; n = 7–8 animals per group.

Mentions: Airway responses to methacholine were assessed by whole-body barometric plethysmography using the Buxco system (Sharon, CT, USA). At baseline, before bromelain or saline treatment (open circles), both groups of animals had similar overall responsiveness (Fig. 5A; P = 0.50) and sensitivity (Fig. 5B) to methacholine (Penh-2 values 48 ± 13 mg/ml in the bromelain treatment versus 64 + 15 mg/ml in saline treatment group; P = 0.47). At AAD (filled circles), saline-treated mice tended to be more generally responsive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B). No change occurred in bromelain-treated 3-day OVA mice terms of overall responsiveness (P = 0.52; A) or methacholine sensitivity (P = 0.45; B).Figure 5.


Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.

Secor ER, Carson WF, Singh A, Pensa M, Guernsey LA, Schramm CM, Thrall RS - Evid Based Complement Alternat Med (2008)

The effect of oral Bromelain treatment on airway reactivity and methacholine sensitivity. At baseline (open circles), Bromelain-treated and saline-treated mice had similar reactivity (P = 0.50) and sensitivity to methacholine (p2 values 48 ± 13 mg/ml in Bromelain treated versus 64 ± 15 mg/ml in saline treated; P = 0.47). After 3 OVA aerosol exposures (filled circles), saline treated mice tended to be more reactive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B) whereas no change occurred in Bromelain treated mice in reactivity (P = 0.52; A) and sensitivity (P = 0.45; B). Comparisons were made by paired t-test and repeated measures ANOVA; n = 7–8 animals per group.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: The effect of oral Bromelain treatment on airway reactivity and methacholine sensitivity. At baseline (open circles), Bromelain-treated and saline-treated mice had similar reactivity (P = 0.50) and sensitivity to methacholine (p2 values 48 ± 13 mg/ml in Bromelain treated versus 64 ± 15 mg/ml in saline treated; P = 0.47). After 3 OVA aerosol exposures (filled circles), saline treated mice tended to be more reactive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B) whereas no change occurred in Bromelain treated mice in reactivity (P = 0.52; A) and sensitivity (P = 0.45; B). Comparisons were made by paired t-test and repeated measures ANOVA; n = 7–8 animals per group.
Mentions: Airway responses to methacholine were assessed by whole-body barometric plethysmography using the Buxco system (Sharon, CT, USA). At baseline, before bromelain or saline treatment (open circles), both groups of animals had similar overall responsiveness (Fig. 5A; P = 0.50) and sensitivity (Fig. 5B) to methacholine (Penh-2 values 48 ± 13 mg/ml in the bromelain treatment versus 64 + 15 mg/ml in saline treatment group; P = 0.47). At AAD (filled circles), saline-treated mice tended to be more generally responsive to methacholine (P = 0.069; A) and were twice as sensitive to methacholine (P = 0.01; B). No change occurred in bromelain-treated 3-day OVA mice terms of overall responsiveness (P = 0.52; A) or methacholine sensitivity (P = 0.45; B).Figure 5.

Bottom Line: Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models.Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P </= 0.01), reduction in BAL eosinophils (P </= 0.02) and IL-13 concentrations (P </= 0.04) as compared with PBS controls.These results suggest that oral treatment with bromelain had a beneficial therapeutic effect in this murine model of asthma and bromelain may also be effective in human conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology and Department of Pediatrics, University of Connecticut School of Medicine, Farmington, CT, USA.

ABSTRACT
Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). To establish AAD, female C57BL/6J mice were sensitized with intraperitoneal (i.p.) OVA/alum and then challenged with OVA aerosols for 3 days. Mice were gavaged with either (phosphate buffered saline)PBS or 200 mg/kg bromelain in PBS, twice daily for four consecutive days, beginning 1 day prior to OVA aerosol challenge. Airway reactivity and methacholine sensitivity, bronchoalveolar lavage (BAL) cellular differential, Th2 cytokines IL-5 and IL-13, and lung histology were compared between treatment groups. Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P

No MeSH data available.


Related in: MedlinePlus