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Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.

Secor ER, Carson WF, Singh A, Pensa M, Guernsey LA, Schramm CM, Thrall RS - Evid Based Complement Alternat Med (2008)

Bottom Line: Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models.Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P </= 0.01), reduction in BAL eosinophils (P </= 0.02) and IL-13 concentrations (P </= 0.04) as compared with PBS controls.These results suggest that oral treatment with bromelain had a beneficial therapeutic effect in this murine model of asthma and bromelain may also be effective in human conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology and Department of Pediatrics, University of Connecticut School of Medicine, Farmington, CT, USA.

ABSTRACT
Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). To establish AAD, female C57BL/6J mice were sensitized with intraperitoneal (i.p.) OVA/alum and then challenged with OVA aerosols for 3 days. Mice were gavaged with either (phosphate buffered saline)PBS or 200 mg/kg bromelain in PBS, twice daily for four consecutive days, beginning 1 day prior to OVA aerosol challenge. Airway reactivity and methacholine sensitivity, bronchoalveolar lavage (BAL) cellular differential, Th2 cytokines IL-5 and IL-13, and lung histology were compared between treatment groups. Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P

No MeSH data available.


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The effect of oral Bromelain treatment on IL-5 and IL-13 concentrations in BAL. There were no significant changes in BAL IL-5 concentrations with oral Bromelain treatment of AAD mice as compared to saline treated AAD mice. A significant decrease in IL-13 concentration was observed in oral Bromealin treated AAD mice as compared to the saline treated AAD mice. Statistical comparisons were made by un-paired t-test, *P ≤ 0.04; Data represent means ± SEM, n = 8 animals per group.
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Figure 4: The effect of oral Bromelain treatment on IL-5 and IL-13 concentrations in BAL. There were no significant changes in BAL IL-5 concentrations with oral Bromelain treatment of AAD mice as compared to saline treated AAD mice. A significant decrease in IL-13 concentration was observed in oral Bromealin treated AAD mice as compared to the saline treated AAD mice. Statistical comparisons were made by un-paired t-test, *P ≤ 0.04; Data represent means ± SEM, n = 8 animals per group.

Mentions: A significant decrease in IL-13 concentration was observed in bromelain-treated AAD mice as compared with the saline treated AAD mice (41 ± 12 versus 86 ± 18 pg/ml; Fig. 4). There were no significant changes in BAL IL-5 concentrations with oral bromelain treatment of AAD mice as compared with saline treated AAD mice (20 ± 4 versus 24 ± 2 pg/ml).Figure 4.


Oral Bromelain Attenuates Inflammation in an Ovalbumin-induced Murine Model of Asthma.

Secor ER, Carson WF, Singh A, Pensa M, Guernsey LA, Schramm CM, Thrall RS - Evid Based Complement Alternat Med (2008)

The effect of oral Bromelain treatment on IL-5 and IL-13 concentrations in BAL. There were no significant changes in BAL IL-5 concentrations with oral Bromelain treatment of AAD mice as compared to saline treated AAD mice. A significant decrease in IL-13 concentration was observed in oral Bromealin treated AAD mice as compared to the saline treated AAD mice. Statistical comparisons were made by un-paired t-test, *P ≤ 0.04; Data represent means ± SEM, n = 8 animals per group.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2249734&req=5

Figure 4: The effect of oral Bromelain treatment on IL-5 and IL-13 concentrations in BAL. There were no significant changes in BAL IL-5 concentrations with oral Bromelain treatment of AAD mice as compared to saline treated AAD mice. A significant decrease in IL-13 concentration was observed in oral Bromealin treated AAD mice as compared to the saline treated AAD mice. Statistical comparisons were made by un-paired t-test, *P ≤ 0.04; Data represent means ± SEM, n = 8 animals per group.
Mentions: A significant decrease in IL-13 concentration was observed in bromelain-treated AAD mice as compared with the saline treated AAD mice (41 ± 12 versus 86 ± 18 pg/ml; Fig. 4). There were no significant changes in BAL IL-5 concentrations with oral bromelain treatment of AAD mice as compared with saline treated AAD mice (20 ± 4 versus 24 ± 2 pg/ml).Figure 4.

Bottom Line: Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models.Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P </= 0.01), reduction in BAL eosinophils (P </= 0.02) and IL-13 concentrations (P </= 0.04) as compared with PBS controls.These results suggest that oral treatment with bromelain had a beneficial therapeutic effect in this murine model of asthma and bromelain may also be effective in human conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology and Department of Pediatrics, University of Connecticut School of Medicine, Farmington, CT, USA.

ABSTRACT
Bromelain, a widely used pineapple extract with cysteine protease activity, has been shown to have immunomodulatory effects in a variety of immune system models. The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). To establish AAD, female C57BL/6J mice were sensitized with intraperitoneal (i.p.) OVA/alum and then challenged with OVA aerosols for 3 days. Mice were gavaged with either (phosphate buffered saline)PBS or 200 mg/kg bromelain in PBS, twice daily for four consecutive days, beginning 1 day prior to OVA aerosol challenge. Airway reactivity and methacholine sensitivity, bronchoalveolar lavage (BAL) cellular differential, Th2 cytokines IL-5 and IL-13, and lung histology were compared between treatment groups. Oral bromelain-treatment of AAD mice demonstrated therapeutic efficacy as evidenced by decreased methacholine sensitivity (P

No MeSH data available.


Related in: MedlinePlus