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Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino Rats.

Chaturvedi P - Evid Based Complement Alternat Med (2008)

Bottom Line: Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group.It also increased the levels of reduced glutathione and increased the activity of catalase.Inclusion of this plant in every day diet would be beneficial.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, University of Botswana, Private bag 0022.

ABSTRACT
In the present study, inhibitory effect of the methanol extract of Raphanus sativus root on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg(-1) body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase. In vitro experiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibited in vitro cumene hydroperoxide induced lipid peroxidation. R. sativus inhibits lipid peroxidation in vivo and in vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

No MeSH data available.


Related in: MedlinePlus

In vitro effect of R. sativus root extract on GSH in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + distilled water + CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE, E3E and E3C compared with NC at 20 and 40 min.
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Figure 2: In vitro effect of R. sativus root extract on GSH in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + distilled water + CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE, E3E and E3C compared with NC at 20 and 40 min.

Mentions: In vitro effect of R. sativus root extract on reduced glutathione in normal and pre-treated rat liver homogenates in CHP-induced peroxidation are presented in Fig. 2. Results show continuous decrease in the levels of reduced glutathione in NC from 0 up to 40 min. In all the three sets of experiment, NE, E3C and E3E, reduced glutathione registers a fall at 10 min, an increase at 20 min and again falls at 40 min. Although the levels of glutathione show increasing and decreasing trends at interval of 10 min in NE, N3C and N3E, their levels show the significant difference from levels in NC at all the observed points and a gradual reduction from 0 min.Figure 2.


Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino Rats.

Chaturvedi P - Evid Based Complement Alternat Med (2008)

In vitro effect of R. sativus root extract on GSH in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + distilled water + CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE, E3E and E3C compared with NC at 20 and 40 min.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2249733&req=5

Figure 2: In vitro effect of R. sativus root extract on GSH in experimental control and experimental treated rat liver homogenate in CHP-induced lipid peroxidation. NC: homogenate from experimental control rats + distilled water + CHP, NE: homogenate from experimental control rats + extract + CHP, E3C: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + distilled water + CHP, E3E: homogenate from experimental treated rats (received 120 mg kg−1 body weight) + extract + CHP. N = 5 in all groups, P < 0.05 when NE, E3E and E3C compared with NC at 20 and 40 min.
Mentions: In vitro effect of R. sativus root extract on reduced glutathione in normal and pre-treated rat liver homogenates in CHP-induced peroxidation are presented in Fig. 2. Results show continuous decrease in the levels of reduced glutathione in NC from 0 up to 40 min. In all the three sets of experiment, NE, E3C and E3E, reduced glutathione registers a fall at 10 min, an increase at 20 min and again falls at 40 min. Although the levels of glutathione show increasing and decreasing trends at interval of 10 min in NE, N3C and N3E, their levels show the significant difference from levels in NC at all the observed points and a gradual reduction from 0 min.Figure 2.

Bottom Line: Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group.It also increased the levels of reduced glutathione and increased the activity of catalase.Inclusion of this plant in every day diet would be beneficial.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Faculty of Science, University of Botswana, Private bag 0022.

ABSTRACT
In the present study, inhibitory effect of the methanol extract of Raphanus sativus root on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg(-1) body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase. In vitro experiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibited in vitro cumene hydroperoxide induced lipid peroxidation. R. sativus inhibits lipid peroxidation in vivo and in vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

No MeSH data available.


Related in: MedlinePlus