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B cell response is required for granuloma formation in the early infection of Schistosoma japonicum.

Ji F, Liu Z, Cao J, Li N, Liu Z, Zuo J, Chen Y, Wang X, Sun J - PLoS ONE (2008)

Bottom Line: Furthermore, defects in B cell function markedly reduce liver egg burden.Surprisingly, we found that the S. japonicum infection destroys the structure of the lymphoid follicles.This disruptive effect is correlated with a severely impaired T cell-dependent antibody response upon challenge with ovalbumin.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Immunology, Institutes of Medical Sciences, Shanghai JiaoTong University School of Medicine, Shanghai, People's Republic of China.

ABSTRACT
Schistosoma egg-induced liver granuloma is a dynamic inflammatory reaction that results from complex immune responses to the infection. However, the role of B cells in inflammatory granuloma development is not yet fully understood. We report here that B cell function is required for S. japonicum egg-induced granuloma pathology in early infection. Both OBF-1 knockout mice and microMT mice develop severely reduced hepatic granulomas at five weeks post-infection compared to their wild-type counterparts. In contrast, they display no significant difference in granuloma pathology at eight weeks post-infection. Moreover, we find that B cells and antibodies accumulate in the granulomas of wild-type mice early in the infection, indicating a contribution of the B cell response to the granulomatous inflammation. Furthermore, defects in B cell function markedly reduce liver egg burden. These results suggest an important role for B cells in early granuloma pathology. Surprisingly, we found that the S. japonicum infection destroys the structure of the lymphoid follicles. This disruptive effect is correlated with a severely impaired T cell-dependent antibody response upon challenge with ovalbumin. Thus, these findings reveal a novel aspect of the interaction between Schistosoma and the host immune system.

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B cell-deficient µMT mice also show markedly impaired granuloma formation at 5 weeks post-infection.(A) Liver granulomas from µMT (n = 9) and C57BL/6 (n = 7) mice were analyzed as described in Fig. 2C. Bars indicate the mean±SD and p values for the compared groups are shown. (B) Liver eggs from mice analyzed in (A) were counted. Data are presented as the mean±SD, and the p values are given.
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pone-0001724-g004: B cell-deficient µMT mice also show markedly impaired granuloma formation at 5 weeks post-infection.(A) Liver granulomas from µMT (n = 9) and C57BL/6 (n = 7) mice were analyzed as described in Fig. 2C. Bars indicate the mean±SD and p values for the compared groups are shown. (B) Liver eggs from mice analyzed in (A) were counted. Data are presented as the mean±SD, and the p values are given.

Mentions: To confirm the role of B cells in the egg-induced granuloma formation in early infection, we studied granuloma development in response to S. japonicum in B cell-deficient µMT mice. S. japonicum-infected µMT mice displayed many fewer hepatic surface nodes than control C57BL/6 mice at five weeks post-infection (data not shown). Quantitative analysis of granuloma formation at five weeks post-infection revealed that the µMT mice developed markedly fewer granulomas in their livers (Fig. 4A). Moreover, the burden of liver eggs in µMT mice was significantly reduced at this stage of infection (Fig. 4B). These data are consistent with our observations in OBF-1- mice. Together, our observations in these two mouse models indicate that a B cell response is required for the development of granulomas early in Schistosoma infection.


B cell response is required for granuloma formation in the early infection of Schistosoma japonicum.

Ji F, Liu Z, Cao J, Li N, Liu Z, Zuo J, Chen Y, Wang X, Sun J - PLoS ONE (2008)

B cell-deficient µMT mice also show markedly impaired granuloma formation at 5 weeks post-infection.(A) Liver granulomas from µMT (n = 9) and C57BL/6 (n = 7) mice were analyzed as described in Fig. 2C. Bars indicate the mean±SD and p values for the compared groups are shown. (B) Liver eggs from mice analyzed in (A) were counted. Data are presented as the mean±SD, and the p values are given.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2248706&req=5

pone-0001724-g004: B cell-deficient µMT mice also show markedly impaired granuloma formation at 5 weeks post-infection.(A) Liver granulomas from µMT (n = 9) and C57BL/6 (n = 7) mice were analyzed as described in Fig. 2C. Bars indicate the mean±SD and p values for the compared groups are shown. (B) Liver eggs from mice analyzed in (A) were counted. Data are presented as the mean±SD, and the p values are given.
Mentions: To confirm the role of B cells in the egg-induced granuloma formation in early infection, we studied granuloma development in response to S. japonicum in B cell-deficient µMT mice. S. japonicum-infected µMT mice displayed many fewer hepatic surface nodes than control C57BL/6 mice at five weeks post-infection (data not shown). Quantitative analysis of granuloma formation at five weeks post-infection revealed that the µMT mice developed markedly fewer granulomas in their livers (Fig. 4A). Moreover, the burden of liver eggs in µMT mice was significantly reduced at this stage of infection (Fig. 4B). These data are consistent with our observations in OBF-1- mice. Together, our observations in these two mouse models indicate that a B cell response is required for the development of granulomas early in Schistosoma infection.

Bottom Line: Furthermore, defects in B cell function markedly reduce liver egg burden.Surprisingly, we found that the S. japonicum infection destroys the structure of the lymphoid follicles.This disruptive effect is correlated with a severely impaired T cell-dependent antibody response upon challenge with ovalbumin.

View Article: PubMed Central - PubMed

Affiliation: Shanghai Institute of Immunology, Institutes of Medical Sciences, Shanghai JiaoTong University School of Medicine, Shanghai, People's Republic of China.

ABSTRACT
Schistosoma egg-induced liver granuloma is a dynamic inflammatory reaction that results from complex immune responses to the infection. However, the role of B cells in inflammatory granuloma development is not yet fully understood. We report here that B cell function is required for S. japonicum egg-induced granuloma pathology in early infection. Both OBF-1 knockout mice and microMT mice develop severely reduced hepatic granulomas at five weeks post-infection compared to their wild-type counterparts. In contrast, they display no significant difference in granuloma pathology at eight weeks post-infection. Moreover, we find that B cells and antibodies accumulate in the granulomas of wild-type mice early in the infection, indicating a contribution of the B cell response to the granulomatous inflammation. Furthermore, defects in B cell function markedly reduce liver egg burden. These results suggest an important role for B cells in early granuloma pathology. Surprisingly, we found that the S. japonicum infection destroys the structure of the lymphoid follicles. This disruptive effect is correlated with a severely impaired T cell-dependent antibody response upon challenge with ovalbumin. Thus, these findings reveal a novel aspect of the interaction between Schistosoma and the host immune system.

Show MeSH
Related in: MedlinePlus