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Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation.

Moghaddami M, Cleland LG, Radisic G, Mayrhofer G - Arthritis Res. Ther. (2007)

Bottom Line: In contrast, typical MHC II(-) monocytes, mainly of the CD4(-) subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells.Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II(-) blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process.However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Arthritis Research Laboratory, Hanson Research Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia, 5000, Australia. mahin.moghaddami@imvs.sa.gov.au

ABSTRACT
Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II(-) monocytes, mainly of the CD4(-) subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b(-), setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4(-) subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II(-) blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.

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Four-color flow cytometry of subsets from synovium-rich tissue (SRT) following adoptive transfer of arthritis. Cell numbers per pair of hind paws (mean, n = 2) were estimated by use of CaliBRITE beads. (a) Numbers of putative dendritic cells (MHC IIhiCD163- cells) with CD4+CD11b+ or CD4-CD11b+ phenotype. (b) Proportions of the same subpopulations. Numbers of CD4- and CD4+ macrophages in the MHC IIhi (c) and MHC IIlo/- (d) subpopulations of CD45+CD163+ cells. (e) Numbers of MHC II-CD45+ cells in SRT after adoptive transfer of arthritis. MHC II- cells with the phenotype CD11b+CD4-CD163- were defined as polymorphonuclear (PMN) cells, whereas those with the phenotypes CD11b+CD4-CD163lo and CD11b+CD4+CD163lo were designated as monocytes. MHC, major histocompatibility complex.
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Figure 5: Four-color flow cytometry of subsets from synovium-rich tissue (SRT) following adoptive transfer of arthritis. Cell numbers per pair of hind paws (mean, n = 2) were estimated by use of CaliBRITE beads. (a) Numbers of putative dendritic cells (MHC IIhiCD163- cells) with CD4+CD11b+ or CD4-CD11b+ phenotype. (b) Proportions of the same subpopulations. Numbers of CD4- and CD4+ macrophages in the MHC IIhi (c) and MHC IIlo/- (d) subpopulations of CD45+CD163+ cells. (e) Numbers of MHC II-CD45+ cells in SRT after adoptive transfer of arthritis. MHC II- cells with the phenotype CD11b+CD4-CD163- were defined as polymorphonuclear (PMN) cells, whereas those with the phenotypes CD11b+CD4-CD163lo and CD11b+CD4+CD163lo were designated as monocytes. MHC, major histocompatibility complex.

Mentions: As a proportion of CD45+MHC IIhi cells, the CD4+CD11b+CD163- and CD4-CD11b+CD163- subsets remained relatively constant throughout adoptively transferred disease (Figure 5b). However, the numbers of cells in both subsets increased approximately 10-fold by day 14 after transfer (Figure 5a). The numbers of MHC IIhiCD11b- cells were also estimated (not shown). The CD4+CD11b- and CD4-CD11b- subsets increased approximately 5- and 10-fold, respectively, by day 14 after transfer but remained unchanged as a proportion of MHC IIhi cells (1% and 5%, respectively).


Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation.

Moghaddami M, Cleland LG, Radisic G, Mayrhofer G - Arthritis Res. Ther. (2007)

Four-color flow cytometry of subsets from synovium-rich tissue (SRT) following adoptive transfer of arthritis. Cell numbers per pair of hind paws (mean, n = 2) were estimated by use of CaliBRITE beads. (a) Numbers of putative dendritic cells (MHC IIhiCD163- cells) with CD4+CD11b+ or CD4-CD11b+ phenotype. (b) Proportions of the same subpopulations. Numbers of CD4- and CD4+ macrophages in the MHC IIhi (c) and MHC IIlo/- (d) subpopulations of CD45+CD163+ cells. (e) Numbers of MHC II-CD45+ cells in SRT after adoptive transfer of arthritis. MHC II- cells with the phenotype CD11b+CD4-CD163- were defined as polymorphonuclear (PMN) cells, whereas those with the phenotypes CD11b+CD4-CD163lo and CD11b+CD4+CD163lo were designated as monocytes. MHC, major histocompatibility complex.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2246239&req=5

Figure 5: Four-color flow cytometry of subsets from synovium-rich tissue (SRT) following adoptive transfer of arthritis. Cell numbers per pair of hind paws (mean, n = 2) were estimated by use of CaliBRITE beads. (a) Numbers of putative dendritic cells (MHC IIhiCD163- cells) with CD4+CD11b+ or CD4-CD11b+ phenotype. (b) Proportions of the same subpopulations. Numbers of CD4- and CD4+ macrophages in the MHC IIhi (c) and MHC IIlo/- (d) subpopulations of CD45+CD163+ cells. (e) Numbers of MHC II-CD45+ cells in SRT after adoptive transfer of arthritis. MHC II- cells with the phenotype CD11b+CD4-CD163- were defined as polymorphonuclear (PMN) cells, whereas those with the phenotypes CD11b+CD4-CD163lo and CD11b+CD4+CD163lo were designated as monocytes. MHC, major histocompatibility complex.
Mentions: As a proportion of CD45+MHC IIhi cells, the CD4+CD11b+CD163- and CD4-CD11b+CD163- subsets remained relatively constant throughout adoptively transferred disease (Figure 5b). However, the numbers of cells in both subsets increased approximately 10-fold by day 14 after transfer (Figure 5a). The numbers of MHC IIhiCD11b- cells were also estimated (not shown). The CD4+CD11b- and CD4-CD11b- subsets increased approximately 5- and 10-fold, respectively, by day 14 after transfer but remained unchanged as a proportion of MHC IIhi cells (1% and 5%, respectively).

Bottom Line: In contrast, typical MHC II(-) monocytes, mainly of the CD4(-) subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells.Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II(-) blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process.However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Arthritis Research Laboratory, Hanson Research Institute, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia, 5000, Australia. mahin.moghaddami@imvs.sa.gov.au

ABSTRACT
Adoptive transfer of adjuvant-induced arthritis was used in this study to examine local macrophages and dendritic cells (DCs) during T cell-mediated synovial inflammation. We studied the influx of CD11b+CD11c+ putative myeloid DCs and other non-lymphoid CD45+ cells into synovium-rich tissues (SRTs) of the affected hind paws in response to a pulse of autoreactive thoracic duct cells. Cells were prepared from the SRTs using a collagenase perfusion-digestion technique, thus allowing enumeration and phenotypic analysis by flow cytometry. Numbers of CD45+ cells increased during the first 6 days, with increases in CD45+MHC (major histocompatibility complex) II+ monocyte-like cells from as early as day 3 after transfer. In contrast, typical MHC II(-) monocytes, mainly of the CD4(-) subset, did not increase until 12 to 14 days after cell transfer, coinciding with the main influx of polymorphonuclear cells. By day 14, CD45+MHC IIhi cells constituted approximately half of all CD45+ cells in SRT. Most of the MHC IIhi cells expressed CD11c and CD11b and represented putative myeloid DCs, whereas only approximately 20% were CD163+ macrophages. Less than 5% of the MHC IIhi cells in inflamed SRT were CD11b(-), setting a maximum for any influx of plasmacytoid DCs. Of the putative myeloid DCs, a third expressed CD4 and both the CD4+ and the CD4(-) subsets expressed the co-stimulatory molecule CD172a. Early accumulation of MHC IIhiCD11c+ monocyte-like cells during the early phase of T cell-mediated inflammation, relative to typical MHC II(-) blood monocytes, suggests that recruited monocytes differentiate rapidly toward the DC lineage at this stage in the disease process. However, it is possible also that the MHC IIhiCD11c+ cells originate from a specific subset of DC-like circulating mononuclear cells.

Show MeSH
Related in: MedlinePlus