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Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb.

Maurer MH, Feldmann RE, Bürgers HF, Kuschinsky W - BMC Neurosci (2008)

Bottom Line: Currently it is unknown whether functional differences in these progenitor cell populations can already be found on the molecular level.We found significant differences in the protein expression patterns between subventricular zone and olfactory bulb neural progenitor cells.No differences in growth characteristics such as doubling time, and passage lengths could be found over 26 consecutive passages in the two cultures.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. maurer@physiologie.uni-heidelberg.de

ABSTRACT

Background: Neural progenitor cells can be isolated from various regions of the adult mammalian brain, including the forebrain structures of the subventricular zone and the olfactory bulb. Currently it is unknown whether functional differences in these progenitor cell populations can already be found on the molecular level. Therefore, we compared protein expression profiles between progenitor cells isolated from the subventricular zone and the olfactory bulb using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry. The subventricular zone and the olfactory bulb are connected by the Rostral Migratory Stream (RMS), in which glial fibrillary acidic protein (GFAP)-positive cells guide neuroblasts. Recent literature suggested that these GFAP-positive cells possess neurogenic potential themselves. In the current study, we therefore compared the cultured neurospheres for the fraction of GFAP-positive cells and their morphology of over a prolonged period of time.

Results: We found significant differences in the protein expression patterns between subventricular zone and olfactory bulb neural progenitor cells. Of the differentially expressed protein spots, 105 were exclusively expressed in the subventricular zone, 23 showed a lower expression and 51 a higher expression in the olfactory bulb. The proteomic data showed that more proteins are differentially expressed in olfactory bulb progenitors with regard to proteins involved in differentiation and microenvironmental integration, as compared to the subventricular zone progenitors. Compared to 94% of all progenitors of the subventricular zone expressed GFAP, nearly none in the olfactory bulb cultures expressed GFAP. Both GFAP-positive subpopulations differed also in morphology, with the olfactory bulb cells showing more branching. No differences in growth characteristics such as doubling time, and passage lengths could be found over 26 consecutive passages in the two cultures.

Conclusion: In this study, we describe differences in protein expression of neural progenitor populations isolated from two forebrain regions, the subventricular zone and the olfactory bulb. These subpopulations can be characterized by differential expression of marker proteins. We isolated fractions of progenitor cells with GFAP expression from both regions, but the GFAP-positive cells differed in number and morphology. Whereas in vitro growth characteristics of neural progenitors are preserved in both regions, our proteomic and immunohistochemical data suggest that progenitor cells from the two regions differ in morphology and functionality, but not in their proliferative capacity.

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Functional categories of the identified proteins. The relative composition of the functional categories varies between the subventricular zone group and the olfactory bulb group. This shift in protein categories may be associated with increased differentiation and integration towards the olfactory bulb. Proteins which have a function in differentiation are highly expressed in undifferentiated cells where differentiation is already initiated. Their expression decreases in terminally differentiated cells, since cellular differentiation programs are not active any more. Therefore, we interpret the decrease in differentiation-related proteins as sign for differentiated, not differentiating cells.
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Figure 2: Functional categories of the identified proteins. The relative composition of the functional categories varies between the subventricular zone group and the olfactory bulb group. This shift in protein categories may be associated with increased differentiation and integration towards the olfactory bulb. Proteins which have a function in differentiation are highly expressed in undifferentiated cells where differentiation is already initiated. Their expression decreases in terminally differentiated cells, since cellular differentiation programs are not active any more. Therefore, we interpret the decrease in differentiation-related proteins as sign for differentiated, not differentiating cells.

Mentions: With regard to the two-dimensional gels, the protein expression pattern of neurospheres from the subventricular zone differs significantly from that of neurospheres from the olfactory bulb (Fig. 2). In order to obtain more specific information on functional aspects, we assigned proteins to several functional categories, mainly cell cycle, differentiation, folding, metabolism, signaling, and transcription. The protein expression pattern from the olfactory bulb cells showed more differentially expressed proteins involved in differentiation and microenvironmental integration.


Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb.

Maurer MH, Feldmann RE, Bürgers HF, Kuschinsky W - BMC Neurosci (2008)

Functional categories of the identified proteins. The relative composition of the functional categories varies between the subventricular zone group and the olfactory bulb group. This shift in protein categories may be associated with increased differentiation and integration towards the olfactory bulb. Proteins which have a function in differentiation are highly expressed in undifferentiated cells where differentiation is already initiated. Their expression decreases in terminally differentiated cells, since cellular differentiation programs are not active any more. Therefore, we interpret the decrease in differentiation-related proteins as sign for differentiated, not differentiating cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2244614&req=5

Figure 2: Functional categories of the identified proteins. The relative composition of the functional categories varies between the subventricular zone group and the olfactory bulb group. This shift in protein categories may be associated with increased differentiation and integration towards the olfactory bulb. Proteins which have a function in differentiation are highly expressed in undifferentiated cells where differentiation is already initiated. Their expression decreases in terminally differentiated cells, since cellular differentiation programs are not active any more. Therefore, we interpret the decrease in differentiation-related proteins as sign for differentiated, not differentiating cells.
Mentions: With regard to the two-dimensional gels, the protein expression pattern of neurospheres from the subventricular zone differs significantly from that of neurospheres from the olfactory bulb (Fig. 2). In order to obtain more specific information on functional aspects, we assigned proteins to several functional categories, mainly cell cycle, differentiation, folding, metabolism, signaling, and transcription. The protein expression pattern from the olfactory bulb cells showed more differentially expressed proteins involved in differentiation and microenvironmental integration.

Bottom Line: Currently it is unknown whether functional differences in these progenitor cell populations can already be found on the molecular level.We found significant differences in the protein expression patterns between subventricular zone and olfactory bulb neural progenitor cells.No differences in growth characteristics such as doubling time, and passage lengths could be found over 26 consecutive passages in the two cultures.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany. maurer@physiologie.uni-heidelberg.de

ABSTRACT

Background: Neural progenitor cells can be isolated from various regions of the adult mammalian brain, including the forebrain structures of the subventricular zone and the olfactory bulb. Currently it is unknown whether functional differences in these progenitor cell populations can already be found on the molecular level. Therefore, we compared protein expression profiles between progenitor cells isolated from the subventricular zone and the olfactory bulb using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry. The subventricular zone and the olfactory bulb are connected by the Rostral Migratory Stream (RMS), in which glial fibrillary acidic protein (GFAP)-positive cells guide neuroblasts. Recent literature suggested that these GFAP-positive cells possess neurogenic potential themselves. In the current study, we therefore compared the cultured neurospheres for the fraction of GFAP-positive cells and their morphology of over a prolonged period of time.

Results: We found significant differences in the protein expression patterns between subventricular zone and olfactory bulb neural progenitor cells. Of the differentially expressed protein spots, 105 were exclusively expressed in the subventricular zone, 23 showed a lower expression and 51 a higher expression in the olfactory bulb. The proteomic data showed that more proteins are differentially expressed in olfactory bulb progenitors with regard to proteins involved in differentiation and microenvironmental integration, as compared to the subventricular zone progenitors. Compared to 94% of all progenitors of the subventricular zone expressed GFAP, nearly none in the olfactory bulb cultures expressed GFAP. Both GFAP-positive subpopulations differed also in morphology, with the olfactory bulb cells showing more branching. No differences in growth characteristics such as doubling time, and passage lengths could be found over 26 consecutive passages in the two cultures.

Conclusion: In this study, we describe differences in protein expression of neural progenitor populations isolated from two forebrain regions, the subventricular zone and the olfactory bulb. These subpopulations can be characterized by differential expression of marker proteins. We isolated fractions of progenitor cells with GFAP expression from both regions, but the GFAP-positive cells differed in number and morphology. Whereas in vitro growth characteristics of neural progenitors are preserved in both regions, our proteomic and immunohistochemical data suggest that progenitor cells from the two regions differ in morphology and functionality, but not in their proliferative capacity.

Show MeSH
Related in: MedlinePlus