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Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana.

Yang L, Tang R, Zhu J, Liu H, Mueller-Roeber B, Xia H, Zhang H - Plant Mol. Biol. (2007)

Bottom Line: Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) in two heterologous systems, i.e. the yeast Saccharomyces cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance.Transgenic tobacco plants constitutively expressing AtIpk2beta under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants.Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2beta in plant stress responses.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.

ABSTRACT
Inositol phosphates (IPs) and their turnover products have been implicated to play important roles in stress signaling in eukaryotic cells. In higher plants genes encoding inositol polyphosphate kinases have been identified previously, but their physiological functions have not been fully resolved. Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) in two heterologous systems, i.e. the yeast Saccharomyces cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance. Expression of AtIpk2beta rescued the salt-, osmotic- and temperature-sensitive growth defects of a yeast mutant strain (arg82Delta) that lacks inositol polyphosphate multikinase activity encoded by the ARG82/IPK2 gene. Transgenic tobacco plants constitutively expressing AtIpk2beta under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants. Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2beta in plant stress responses.

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Effect of drought and osmotic stress on plant performance. For each experiment, twelve plants of wild type (WT) and each transgenic line (S13, S15) were used. (a) Wild type and transgenic plants grown in soil were watered with 60 g/l PEG-6000. Photograph was taken after 21 days of treatment. (b) Water was withheld for 21 days to impose drought stress. (c) Drought stressed plants were re-watered and grown for seed setting. (d) Proline content of wild type and transgenic plants after treatment with 100 or 200 mM NaCl for 24 h. Results are presented as means and standard errors from three independent experiments. ** indicates significant differences in comparison to the wild type at P < 0.01 (Student’s t-test)
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Fig5: Effect of drought and osmotic stress on plant performance. For each experiment, twelve plants of wild type (WT) and each transgenic line (S13, S15) were used. (a) Wild type and transgenic plants grown in soil were watered with 60 g/l PEG-6000. Photograph was taken after 21 days of treatment. (b) Water was withheld for 21 days to impose drought stress. (c) Drought stressed plants were re-watered and grown for seed setting. (d) Proline content of wild type and transgenic plants after treatment with 100 or 200 mM NaCl for 24 h. Results are presented as means and standard errors from three independent experiments. ** indicates significant differences in comparison to the wild type at P < 0.01 (Student’s t-test)

Mentions: In order to determine whether expression of AtIpk2β affects osmotic stress tolerance in plants, we watered wild type and transgenic tobacco plants (lines S13 and S15) with PEG-6000 (60 g/l) as described previously (Eltayeb et al. 2006). Under such experimental conditions, wild type plants were less vigorous, flowered earlier and produced smaller leaves than AtIpk2β expressing plants (Fig. 5a). We also tested the effect of water shortage on plant growth. After a 21-day drought period, wild type plants completely wilted, while transgenic plants were less severely affected (Fig. 5b). At the end of the drought tolerance test, the plants were re-watered and grown further to allow seed setting. Transgenic plants produced larger shoots than wild type plants (Fig. 5c).Fig. 5


Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana.

Yang L, Tang R, Zhu J, Liu H, Mueller-Roeber B, Xia H, Zhang H - Plant Mol. Biol. (2007)

Effect of drought and osmotic stress on plant performance. For each experiment, twelve plants of wild type (WT) and each transgenic line (S13, S15) were used. (a) Wild type and transgenic plants grown in soil were watered with 60 g/l PEG-6000. Photograph was taken after 21 days of treatment. (b) Water was withheld for 21 days to impose drought stress. (c) Drought stressed plants were re-watered and grown for seed setting. (d) Proline content of wild type and transgenic plants after treatment with 100 or 200 mM NaCl for 24 h. Results are presented as means and standard errors from three independent experiments. ** indicates significant differences in comparison to the wild type at P < 0.01 (Student’s t-test)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2238787&req=5

Fig5: Effect of drought and osmotic stress on plant performance. For each experiment, twelve plants of wild type (WT) and each transgenic line (S13, S15) were used. (a) Wild type and transgenic plants grown in soil were watered with 60 g/l PEG-6000. Photograph was taken after 21 days of treatment. (b) Water was withheld for 21 days to impose drought stress. (c) Drought stressed plants were re-watered and grown for seed setting. (d) Proline content of wild type and transgenic plants after treatment with 100 or 200 mM NaCl for 24 h. Results are presented as means and standard errors from three independent experiments. ** indicates significant differences in comparison to the wild type at P < 0.01 (Student’s t-test)
Mentions: In order to determine whether expression of AtIpk2β affects osmotic stress tolerance in plants, we watered wild type and transgenic tobacco plants (lines S13 and S15) with PEG-6000 (60 g/l) as described previously (Eltayeb et al. 2006). Under such experimental conditions, wild type plants were less vigorous, flowered earlier and produced smaller leaves than AtIpk2β expressing plants (Fig. 5a). We also tested the effect of water shortage on plant growth. After a 21-day drought period, wild type plants completely wilted, while transgenic plants were less severely affected (Fig. 5b). At the end of the drought tolerance test, the plants were re-watered and grown further to allow seed setting. Transgenic plants produced larger shoots than wild type plants (Fig. 5c).Fig. 5

Bottom Line: Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) in two heterologous systems, i.e. the yeast Saccharomyces cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance.Transgenic tobacco plants constitutively expressing AtIpk2beta under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants.Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2beta in plant stress responses.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.

ABSTRACT
Inositol phosphates (IPs) and their turnover products have been implicated to play important roles in stress signaling in eukaryotic cells. In higher plants genes encoding inositol polyphosphate kinases have been identified previously, but their physiological functions have not been fully resolved. Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2beta) in two heterologous systems, i.e. the yeast Saccharomyces cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance. Expression of AtIpk2beta rescued the salt-, osmotic- and temperature-sensitive growth defects of a yeast mutant strain (arg82Delta) that lacks inositol polyphosphate multikinase activity encoded by the ARG82/IPK2 gene. Transgenic tobacco plants constitutively expressing AtIpk2beta under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants. Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2beta in plant stress responses.

Show MeSH
Related in: MedlinePlus