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The enhanced expression of the matrix metalloproteinase 9 in nasal NK/T-cell lymphoma.

Sakata K, Someya M, Omatsu M, Asanuma H, Hasegawa T, Ichimiya S, Hareyama M, Himi T - BMC Cancer (2007)

Bottom Line: When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them.The difference was statistically significant (p = 0.027).However, our results are based on univariate comparisons, and as such, should be viewed with some caution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiology, Sapporo Medical University, School of Medicine, Sapporo, Japan. sakatako@sapmed.ac.jp

ABSTRACT

Background: Nasal NK/T cell lymphoma is an aggressive disease and has a poor prognosis. Nasal NK/T cell lymphoma is refractory to conventional chemotherapy and has strong tendency of widespread relapse or dissemination into distant sites.

Methods: We immunohistochemically studied nasal NK/T-cell lymphoma to elucidate the unique characteristics of nasal NK/T-cell lymphoma, such as its higher metastatic tendency and its vast necrosis which leads to destruction of the involved tissues. The expression of P-glycoprotein and MMP-9 was evaluated in the 20 patients with nasal NK/T-cell lymphoma and 25 with nasal non-NK/T-cell lymphoma and the relationship between expression of these proteins and clinical results were analyzed in this report.

Results: Overall 5-year survival rates for patients with nasal NK/T cell lymphoma, and nasal non-NK/T cell lymphoma were 51%, and 84%. Distant involvement free 5-year survival rates for patients with nasal NK/T cell lymphoma, and nasal non-NK/T cell lymphoma were 53%, and 79%. Overall positivity for P-glycoprotein was observed in 10 of 19 patients with NTL and in 13 of 23 patients with non-NTL. When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them. Sixteen of the 19 patients with nasal NK/T cell lymphoma expressed MMP-9. In contrast, only 8 of the 22 patients with nasal non-NK/T cell lymphoma expressed MMP-9. Distant involvement free 5-year survival rates for patients with MMP-9 negative, and MMP-9 positive were 92%, and 61%, respectively. The difference was statistically significant (p = 0.027).

Conclusion: Positive immunoreactivity for P-glycoprotein was not an independent prognostic factor in nasal NK/T-cell lymphomas, which stresses the importance of exploring other mechanisms of drug resistance. The strong expression of MMP-9 is uniquely characteristic of nasal NK/T cell lymphoma and may contribute to its strong tendency to disseminatate and the extensive necrosis which is always seen. However, our results are based on univariate comparisons, and as such, should be viewed with some caution.

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(a) Expression of P-glycoprotein in nasal NK/T cell lymphoma (original magnification, ×200). (b) Expression of MMP-9 in nasal NK/T cell lymphoma (original magnification, ×200).
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Figure 3: (a) Expression of P-glycoprotein in nasal NK/T cell lymphoma (original magnification, ×200). (b) Expression of MMP-9 in nasal NK/T cell lymphoma (original magnification, ×200).

Mentions: Within malignant cells, patterns of P-glycoprotein immunoreactivity generally were of membrane staining, but cytoplasmic immunoreactivity was occasionally observed in some tumor cells (Fig. 3a). When a staining distribution cutoff value of greater than 10% was used to classify positive immunoreactivity, overall positivity for P-glycoprotein was observed in 10 of 19 patients with NTL and in 13 of 23 patients with non-NTL (Table 2). When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them (p = 0.88) (Fig. 4). The similar result was obtained, when this analysis was also done separately for T and B cell lymphomas. The 5-year survival rate for P-glycoprotein positive and negative in T cell lymphomas was 68% and 67%. The 5-year survival rate for P-glycoprotein positive and negative in B cell lymphomas was 85% and 71%.


The enhanced expression of the matrix metalloproteinase 9 in nasal NK/T-cell lymphoma.

Sakata K, Someya M, Omatsu M, Asanuma H, Hasegawa T, Ichimiya S, Hareyama M, Himi T - BMC Cancer (2007)

(a) Expression of P-glycoprotein in nasal NK/T cell lymphoma (original magnification, ×200). (b) Expression of MMP-9 in nasal NK/T cell lymphoma (original magnification, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2238761&req=5

Figure 3: (a) Expression of P-glycoprotein in nasal NK/T cell lymphoma (original magnification, ×200). (b) Expression of MMP-9 in nasal NK/T cell lymphoma (original magnification, ×200).
Mentions: Within malignant cells, patterns of P-glycoprotein immunoreactivity generally were of membrane staining, but cytoplasmic immunoreactivity was occasionally observed in some tumor cells (Fig. 3a). When a staining distribution cutoff value of greater than 10% was used to classify positive immunoreactivity, overall positivity for P-glycoprotein was observed in 10 of 19 patients with NTL and in 13 of 23 patients with non-NTL (Table 2). When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them (p = 0.88) (Fig. 4). The similar result was obtained, when this analysis was also done separately for T and B cell lymphomas. The 5-year survival rate for P-glycoprotein positive and negative in T cell lymphomas was 68% and 67%. The 5-year survival rate for P-glycoprotein positive and negative in B cell lymphomas was 85% and 71%.

Bottom Line: When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them.The difference was statistically significant (p = 0.027).However, our results are based on univariate comparisons, and as such, should be viewed with some caution.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Radiology, Sapporo Medical University, School of Medicine, Sapporo, Japan. sakatako@sapmed.ac.jp

ABSTRACT

Background: Nasal NK/T cell lymphoma is an aggressive disease and has a poor prognosis. Nasal NK/T cell lymphoma is refractory to conventional chemotherapy and has strong tendency of widespread relapse or dissemination into distant sites.

Methods: We immunohistochemically studied nasal NK/T-cell lymphoma to elucidate the unique characteristics of nasal NK/T-cell lymphoma, such as its higher metastatic tendency and its vast necrosis which leads to destruction of the involved tissues. The expression of P-glycoprotein and MMP-9 was evaluated in the 20 patients with nasal NK/T-cell lymphoma and 25 with nasal non-NK/T-cell lymphoma and the relationship between expression of these proteins and clinical results were analyzed in this report.

Results: Overall 5-year survival rates for patients with nasal NK/T cell lymphoma, and nasal non-NK/T cell lymphoma were 51%, and 84%. Distant involvement free 5-year survival rates for patients with nasal NK/T cell lymphoma, and nasal non-NK/T cell lymphoma were 53%, and 79%. Overall positivity for P-glycoprotein was observed in 10 of 19 patients with NTL and in 13 of 23 patients with non-NTL. When the overall survival rate was compared between patients with P-glycoprotein positive and negative, there was no difference between them. Sixteen of the 19 patients with nasal NK/T cell lymphoma expressed MMP-9. In contrast, only 8 of the 22 patients with nasal non-NK/T cell lymphoma expressed MMP-9. Distant involvement free 5-year survival rates for patients with MMP-9 negative, and MMP-9 positive were 92%, and 61%, respectively. The difference was statistically significant (p = 0.027).

Conclusion: Positive immunoreactivity for P-glycoprotein was not an independent prognostic factor in nasal NK/T-cell lymphomas, which stresses the importance of exploring other mechanisms of drug resistance. The strong expression of MMP-9 is uniquely characteristic of nasal NK/T cell lymphoma and may contribute to its strong tendency to disseminatate and the extensive necrosis which is always seen. However, our results are based on univariate comparisons, and as such, should be viewed with some caution.

Show MeSH
Related in: MedlinePlus