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Transgenic mice expressing constitutive active MAPKAPK5 display gender-dependent differences in exploration and activity.

Gerits N, Van Belle W, Moens U - Behav Brain Funct (2007)

Bottom Line: Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Virology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N-9037 Tromsø, Norway. nancy@sigtrans.org.

ABSTRACT

Background: The mitogen-activated protein kinases, MAPKs for short, constitute cascades of signalling pathways involved in the regulation of several cellular processes that include cell proliferation, differentiation and motility. They also intervene in neurological processes like fear conditioning and memory. Since little remains known about the MAPK-Activated Protein Kinase, MAPKAPK5, we constructed the first MAPKAPK knockin mouse model, using a constitutive active variant of MAPKAPK5 and analyzed the resulting mice for changes in anxiety-related behaviour.

Methods: We performed primary SHIRPA observations during background breeding into the C57BL/6 background and assessed the behaviour of the background-bred animals on the elevated plus maze and in the light-dark test. Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.

Results: Female transgenic mice displayed increased amounts of head dips and open arm time on the maze, compared to littermate controls. In addition, they also explored further into the open arm on the elevated plus maze and were less active in the closed arm compared to littermate controls. Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.

Conclusion: Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

No MeSH data available.


Related in: MedlinePlus

Overview of the mouse activity profiles in the EPM. Each graph represents, the activity in the open and closed arms for TNFG (panel A), FTG (panel B), MNTG (panel C) and MTG (panel D). Mouse activity is indicated in colour with red being highest activity, whereas blue reflects low mouse activity in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively.
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Figure 4: Overview of the mouse activity profiles in the EPM. Each graph represents, the activity in the open and closed arms for TNFG (panel A), FTG (panel B), MNTG (panel C) and MTG (panel D). Mouse activity is indicated in colour with red being highest activity, whereas blue reflects low mouse activity in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively.

Mentions: Analysis of the total entries, the entries into the closed arms and rearings may give an indication on the activity of the mice [20]. As Table 2 shows, we observed a 3.17% (p = 0.05) reduction in time spent in the closed arm between TG and NTG mice. This difference accounts for the increased percentage of time spent in the open arm of the EPM by FTG. For FNTG and FTG mice, the centre time did not differ significantly, whereas MTG spent less time there than MNTG. The mouse presence probabilities in the closed arms (Figure 3) revealed no overt differences between MNTG and MTG mice (Figure 3C and 3D), but as the white box indicates, they did display a difference between FTG and FNTG mice (Figure 3A and 3B). Apparently, FTG spent less time in exploring the centre of the closed arm than FNTG mice. They may compensate the lack of exploration in the centre by their presence near the extreme end of the closed arm or near the centre of the maze (compare Figure 3A and 3B). Our activity profiles in Figure 4 divulged no obvious differences in activity between FTG, FNTG, MNTG and MTG mice and confirmed the results from the time spent and entries into the arms of the maze.


Transgenic mice expressing constitutive active MAPKAPK5 display gender-dependent differences in exploration and activity.

Gerits N, Van Belle W, Moens U - Behav Brain Funct (2007)

Overview of the mouse activity profiles in the EPM. Each graph represents, the activity in the open and closed arms for TNFG (panel A), FTG (panel B), MNTG (panel C) and MTG (panel D). Mouse activity is indicated in colour with red being highest activity, whereas blue reflects low mouse activity in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2238755&req=5

Figure 4: Overview of the mouse activity profiles in the EPM. Each graph represents, the activity in the open and closed arms for TNFG (panel A), FTG (panel B), MNTG (panel C) and MTG (panel D). Mouse activity is indicated in colour with red being highest activity, whereas blue reflects low mouse activity in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively.
Mentions: Analysis of the total entries, the entries into the closed arms and rearings may give an indication on the activity of the mice [20]. As Table 2 shows, we observed a 3.17% (p = 0.05) reduction in time spent in the closed arm between TG and NTG mice. This difference accounts for the increased percentage of time spent in the open arm of the EPM by FTG. For FNTG and FTG mice, the centre time did not differ significantly, whereas MTG spent less time there than MNTG. The mouse presence probabilities in the closed arms (Figure 3) revealed no overt differences between MNTG and MTG mice (Figure 3C and 3D), but as the white box indicates, they did display a difference between FTG and FNTG mice (Figure 3A and 3B). Apparently, FTG spent less time in exploring the centre of the closed arm than FNTG mice. They may compensate the lack of exploration in the centre by their presence near the extreme end of the closed arm or near the centre of the maze (compare Figure 3A and 3B). Our activity profiles in Figure 4 divulged no obvious differences in activity between FTG, FNTG, MNTG and MTG mice and confirmed the results from the time spent and entries into the arms of the maze.

Bottom Line: Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Virology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N-9037 Tromsø, Norway. nancy@sigtrans.org.

ABSTRACT

Background: The mitogen-activated protein kinases, MAPKs for short, constitute cascades of signalling pathways involved in the regulation of several cellular processes that include cell proliferation, differentiation and motility. They also intervene in neurological processes like fear conditioning and memory. Since little remains known about the MAPK-Activated Protein Kinase, MAPKAPK5, we constructed the first MAPKAPK knockin mouse model, using a constitutive active variant of MAPKAPK5 and analyzed the resulting mice for changes in anxiety-related behaviour.

Methods: We performed primary SHIRPA observations during background breeding into the C57BL/6 background and assessed the behaviour of the background-bred animals on the elevated plus maze and in the light-dark test. Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.

Results: Female transgenic mice displayed increased amounts of head dips and open arm time on the maze, compared to littermate controls. In addition, they also explored further into the open arm on the elevated plus maze and were less active in the closed arm compared to littermate controls. Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.

Conclusion: Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

No MeSH data available.


Related in: MedlinePlus