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Transgenic mice expressing constitutive active MAPKAPK5 display gender-dependent differences in exploration and activity.

Gerits N, Van Belle W, Moens U - Behav Brain Funct (2007)

Bottom Line: Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Virology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N-9037 Tromsø, Norway. nancy@sigtrans.org.

ABSTRACT

Background: The mitogen-activated protein kinases, MAPKs for short, constitute cascades of signalling pathways involved in the regulation of several cellular processes that include cell proliferation, differentiation and motility. They also intervene in neurological processes like fear conditioning and memory. Since little remains known about the MAPK-Activated Protein Kinase, MAPKAPK5, we constructed the first MAPKAPK knockin mouse model, using a constitutive active variant of MAPKAPK5 and analyzed the resulting mice for changes in anxiety-related behaviour.

Methods: We performed primary SHIRPA observations during background breeding into the C57BL/6 background and assessed the behaviour of the background-bred animals on the elevated plus maze and in the light-dark test. Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.

Results: Female transgenic mice displayed increased amounts of head dips and open arm time on the maze, compared to littermate controls. In addition, they also explored further into the open arm on the elevated plus maze and were less active in the closed arm compared to littermate controls. Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.

Conclusion: Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

No MeSH data available.


Related in: MedlinePlus

Overview of the mouse presence probability on the EPM over a 5 minute period. Each graph represents for each gender in a specific genetic background, where on the maze the mice are present most. Increased presence is indicated in red, whereas blue reflects a lower probability to find the mouse in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively. The sharp mouse contour in the middle of the open arm in graph A and at the end of the open arm in graph C illustrates that one mouse resided there for a long period. This mouse is considered an outlier, since it does not represent the rest of that mouse population. Since this graph is a superposition of each population, a more representative sample would be indicated by more blurry edges.
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Figure 3: Overview of the mouse presence probability on the EPM over a 5 minute period. Each graph represents for each gender in a specific genetic background, where on the maze the mice are present most. Increased presence is indicated in red, whereas blue reflects a lower probability to find the mouse in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively. The sharp mouse contour in the middle of the open arm in graph A and at the end of the open arm in graph C illustrates that one mouse resided there for a long period. This mouse is considered an outlier, since it does not represent the rest of that mouse population. Since this graph is a superposition of each population, a more representative sample would be indicated by more blurry edges.

Mentions: In support of these findings, our image analysis indicates that FTG mice explored further into the open arm compared to NTG littermates (Figures 3A and 3B). This figure also illustrates a FNTG mouse shape in the middle of the open arm. Since our imaging technique uses superposition of the images to acquire a general distribution, the more general the behaviour is, the more blurrier shapes become. Therefore, the sharp contours of the mouse in Figure 3A indicate it is an outlier. This contrasts to Figure 3B, where the mouse presence is more extended into the open arm and the shape is more blurred. We did not remove the outlier because its presence or absence does not influence the end result. We noticed a similar, though slightly more blurred shape in the MNTG mouse at the end of the open arm in Figure 3C. Aside from this animal, the mouse presence probability between MNTG and MTG is very similar. In Figure 3C and 3D, the white boxes near the beginning of the open arm indicate a similar contribution of the mouse presence in MNTG and MTG mice, with the red area in Figure 3D compensating for the more extended area in Figure 3C. However, we can distinguish that MNTG ventured further into the open arm compared to MTG mice.


Transgenic mice expressing constitutive active MAPKAPK5 display gender-dependent differences in exploration and activity.

Gerits N, Van Belle W, Moens U - Behav Brain Funct (2007)

Overview of the mouse presence probability on the EPM over a 5 minute period. Each graph represents for each gender in a specific genetic background, where on the maze the mice are present most. Increased presence is indicated in red, whereas blue reflects a lower probability to find the mouse in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively. The sharp mouse contour in the middle of the open arm in graph A and at the end of the open arm in graph C illustrates that one mouse resided there for a long period. This mouse is considered an outlier, since it does not represent the rest of that mouse population. Since this graph is a superposition of each population, a more representative sample would be indicated by more blurry edges.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2238755&req=5

Figure 3: Overview of the mouse presence probability on the EPM over a 5 minute period. Each graph represents for each gender in a specific genetic background, where on the maze the mice are present most. Increased presence is indicated in red, whereas blue reflects a lower probability to find the mouse in that area. The X-axis and Y-axis illustrate the superposition of the two open and closed arms respectively. The sharp mouse contour in the middle of the open arm in graph A and at the end of the open arm in graph C illustrates that one mouse resided there for a long period. This mouse is considered an outlier, since it does not represent the rest of that mouse population. Since this graph is a superposition of each population, a more representative sample would be indicated by more blurry edges.
Mentions: In support of these findings, our image analysis indicates that FTG mice explored further into the open arm compared to NTG littermates (Figures 3A and 3B). This figure also illustrates a FNTG mouse shape in the middle of the open arm. Since our imaging technique uses superposition of the images to acquire a general distribution, the more general the behaviour is, the more blurrier shapes become. Therefore, the sharp contours of the mouse in Figure 3A indicate it is an outlier. This contrasts to Figure 3B, where the mouse presence is more extended into the open arm and the shape is more blurred. We did not remove the outlier because its presence or absence does not influence the end result. We noticed a similar, though slightly more blurred shape in the MNTG mouse at the end of the open arm in Figure 3C. Aside from this animal, the mouse presence probability between MNTG and MTG is very similar. In Figure 3C and 3D, the white boxes near the beginning of the open arm indicate a similar contribution of the mouse presence in MNTG and MTG mice, with the red area in Figure 3D compensating for the more extended area in Figure 3C. However, we can distinguish that MNTG ventured further into the open arm compared to MTG mice.

Bottom Line: Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology and Virology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, N-9037 Tromsø, Norway. nancy@sigtrans.org.

ABSTRACT

Background: The mitogen-activated protein kinases, MAPKs for short, constitute cascades of signalling pathways involved in the regulation of several cellular processes that include cell proliferation, differentiation and motility. They also intervene in neurological processes like fear conditioning and memory. Since little remains known about the MAPK-Activated Protein Kinase, MAPKAPK5, we constructed the first MAPKAPK knockin mouse model, using a constitutive active variant of MAPKAPK5 and analyzed the resulting mice for changes in anxiety-related behaviour.

Methods: We performed primary SHIRPA observations during background breeding into the C57BL/6 background and assessed the behaviour of the background-bred animals on the elevated plus maze and in the light-dark test. Our results were analyzed using Chi-square tests and homo- and heteroscedatic T-tests.

Results: Female transgenic mice displayed increased amounts of head dips and open arm time on the maze, compared to littermate controls. In addition, they also explored further into the open arm on the elevated plus maze and were less active in the closed arm compared to littermate controls. Male transgenic mice displayed no differences in anxiety, but their locomotor activity increased compared to non-transgenic littermates.

Conclusion: Our results revealed anxiety-related traits and locomotor differences between transgenic mice expressing constitutive active MAPKAPK5 and control littermates.

No MeSH data available.


Related in: MedlinePlus