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Reduced white fat mass in adult mice bearing a truncated Patched 1.

Li Z, Zhang H, Denhard LA, Liu LH, Zhou H, Lan ZJ - Int. J. Biol. Sci. (2008)

Bottom Line: Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development.Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1(mes/mes)), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region.Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.

View Article: PubMed Central - PubMed

Affiliation: Birth Defects Center, Department of Molecular, Cellular, Craniofacial Biology, University of Louisville Health Sciences Center, Louisville, KY 40202, USA.

ABSTRACT
Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development. In this report, we found that Patched 1 (Ptc1), a negative regulator of Hh signaling, was expressed in the epididymal fat pad of adult mice. Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1(mes/mes)), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region. Increased expression of truncated Ptc1, Ptc2 and Gli1, the indicators of ectopic activation of Hh signaling, was observed in epididymal fat pads of adult Ptc1(mes/mes) mice. In contrast, expression of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha, adipocyte P2 and adipsin were reduced in epididymal fat pads of adult Ptc1(mes/mes) mice. Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.

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Expression of Ptc1, Ptc2, Gli1 Gli2 and Gli3 in mouse epididymal WAT. Epididymal WAT RNA from 8-week-old mice was subjected to RT-PCR analyses using specific primers for Ptc1 (Ptc1-F and Ptc1-R), Ptc2, Gli1, Gli2, Gli3, Gilz, PPARgamma, CEBPalpha, aP2 and adipsin. The numbers of PCR reaction cycles are 36 for Ptc1, 38 for Ptc2 and 32 for other tested genes. Water was included as negative controls.
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Figure 1: Expression of Ptc1, Ptc2, Gli1 Gli2 and Gli3 in mouse epididymal WAT. Epididymal WAT RNA from 8-week-old mice was subjected to RT-PCR analyses using specific primers for Ptc1 (Ptc1-F and Ptc1-R), Ptc2, Gli1, Gli2, Gli3, Gilz, PPARgamma, CEBPalpha, aP2 and adipsin. The numbers of PCR reaction cycles are 36 for Ptc1, 38 for Ptc2 and 32 for other tested genes. Water was included as negative controls.

Mentions: To determine whether Hh signaling molecules such as Ptc1, Ptc2, Gli1, Gli2 and Gli3 are expressed in WAT of adult mice, we performed RT-PCR analyses using specific primers for each gene. As shown in Figure 1, specific PCR products for those genes were detected in RNA samples from epididymal WAT of adult mice. In addition, we also observed the expression of an antiadipogenic gene Gilz 27, adipogenic genes CEBPalpha and PPARgamma, and mature adipocyte marker genes aP2 and adipsin in epididymal WAT (Figure 1).


Reduced white fat mass in adult mice bearing a truncated Patched 1.

Li Z, Zhang H, Denhard LA, Liu LH, Zhou H, Lan ZJ - Int. J. Biol. Sci. (2008)

Expression of Ptc1, Ptc2, Gli1 Gli2 and Gli3 in mouse epididymal WAT. Epididymal WAT RNA from 8-week-old mice was subjected to RT-PCR analyses using specific primers for Ptc1 (Ptc1-F and Ptc1-R), Ptc2, Gli1, Gli2, Gli3, Gilz, PPARgamma, CEBPalpha, aP2 and adipsin. The numbers of PCR reaction cycles are 36 for Ptc1, 38 for Ptc2 and 32 for other tested genes. Water was included as negative controls.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2238183&req=5

Figure 1: Expression of Ptc1, Ptc2, Gli1 Gli2 and Gli3 in mouse epididymal WAT. Epididymal WAT RNA from 8-week-old mice was subjected to RT-PCR analyses using specific primers for Ptc1 (Ptc1-F and Ptc1-R), Ptc2, Gli1, Gli2, Gli3, Gilz, PPARgamma, CEBPalpha, aP2 and adipsin. The numbers of PCR reaction cycles are 36 for Ptc1, 38 for Ptc2 and 32 for other tested genes. Water was included as negative controls.
Mentions: To determine whether Hh signaling molecules such as Ptc1, Ptc2, Gli1, Gli2 and Gli3 are expressed in WAT of adult mice, we performed RT-PCR analyses using specific primers for each gene. As shown in Figure 1, specific PCR products for those genes were detected in RNA samples from epididymal WAT of adult mice. In addition, we also observed the expression of an antiadipogenic gene Gilz 27, adipogenic genes CEBPalpha and PPARgamma, and mature adipocyte marker genes aP2 and adipsin in epididymal WAT (Figure 1).

Bottom Line: Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development.Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1(mes/mes)), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region.Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.

View Article: PubMed Central - PubMed

Affiliation: Birth Defects Center, Department of Molecular, Cellular, Craniofacial Biology, University of Louisville Health Sciences Center, Louisville, KY 40202, USA.

ABSTRACT
Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development. In this report, we found that Patched 1 (Ptc1), a negative regulator of Hh signaling, was expressed in the epididymal fat pad of adult mice. Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1(mes/mes)), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region. Increased expression of truncated Ptc1, Ptc2 and Gli1, the indicators of ectopic activation of Hh signaling, was observed in epididymal fat pads of adult Ptc1(mes/mes) mice. In contrast, expression of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha, adipocyte P2 and adipsin were reduced in epididymal fat pads of adult Ptc1(mes/mes) mice. Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.

Show MeSH
Related in: MedlinePlus