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Protecting effects of dexamethasone on thymus of rats with severe acute pancreatitis.

Xiping Z, Li C, Miao L, Hua T - Mediators Inflamm. (2007)

Bottom Line: The contents of different indexes in blood of treated group were lower than those of the model group to various degrees at different time points.The treated group in Caspase-3 protein expression of thymus significantly exceeded the model group at 12 hours.The apoptotic index was significantly higher in treated group than in model group.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Hangzhou First People's Hospital, Hangzhou 310006, Zhejiang Province, China. zxp99688@vip.163.com

ABSTRACT

Purpose: To study the protecting effects of dexamethasone on thymus of rats with severe acute pancreatitis (SAP).

Methods: The SAP rats were randomly assigned to the model group and dexamethasone-treated group, the other normal healthy rats were assigned to the sham operation group. The rat survival, thymus pathological changes, apoptotic index, as well as expression levels of NF-kappaB, P-selectin, Bax, Bcl-2, and Caspase-3 protein of all groups were observed, respectively, at 3 hours, 6 hours, and 12 hours. The contents of amylase and endotoxin in plasma as well as the contents of TNF-alpha, PLA2, and NO in serum were determined.

Results: There was no marked difference between the model group and treated group in survival. The contents of different indexes in blood of treated group were lower than those of the model group to various degrees at different time points. The thymus pathological score was lower in treated group than in model group at 12 hours. The treated group in Caspase-3 protein expression of thymus significantly exceeded the model group at 12 hours. The apoptotic index was significantly higher in treated group than in model group.

Conclusion: Dexamethasone has protecting effects on thymus of SAP rats.

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Related in: MedlinePlus

Sham operation group: 6 hours; Tunel × 200.
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Related In: Results  -  Collection


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fig2: Sham operation group: 6 hours; Tunel × 200.

Mentions: Lymphocytes were apoptotic cells ofthymus. In sham operation group, apoptotic cells were found, respectively, in 2and 1 rat at 6 hours and 12 hours, and the apoptotic index was between 10 per10000 and 24 per 10000 as shown in Figures 2 and 3. In model group,apoptotic cells were found in 2 rats at 12 hours, and the apoptotic index wasbetween 6 per 10000 and 8 per 10000 as shown in Figure 4. In treated group,apoptotic cells were found, respectively, in 6, 9, and 8 rats at 3 hours, 6 hours,and 12 hours, and the apoptotic index was between 2 per 10000 and 76 per 10000as shown in Figures 5 and 6. The counts were higher in treated group than in shamoperation group and model group at different time points (P < .05).There was no marked difference between model group and sham operation group (P > .05), see Table 5.


Protecting effects of dexamethasone on thymus of rats with severe acute pancreatitis.

Xiping Z, Li C, Miao L, Hua T - Mediators Inflamm. (2007)

Sham operation group: 6 hours; Tunel × 200.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2234334&req=5

fig2: Sham operation group: 6 hours; Tunel × 200.
Mentions: Lymphocytes were apoptotic cells ofthymus. In sham operation group, apoptotic cells were found, respectively, in 2and 1 rat at 6 hours and 12 hours, and the apoptotic index was between 10 per10000 and 24 per 10000 as shown in Figures 2 and 3. In model group,apoptotic cells were found in 2 rats at 12 hours, and the apoptotic index wasbetween 6 per 10000 and 8 per 10000 as shown in Figure 4. In treated group,apoptotic cells were found, respectively, in 6, 9, and 8 rats at 3 hours, 6 hours,and 12 hours, and the apoptotic index was between 2 per 10000 and 76 per 10000as shown in Figures 5 and 6. The counts were higher in treated group than in shamoperation group and model group at different time points (P < .05).There was no marked difference between model group and sham operation group (P > .05), see Table 5.

Bottom Line: The contents of different indexes in blood of treated group were lower than those of the model group to various degrees at different time points.The treated group in Caspase-3 protein expression of thymus significantly exceeded the model group at 12 hours.The apoptotic index was significantly higher in treated group than in model group.

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Hangzhou First People's Hospital, Hangzhou 310006, Zhejiang Province, China. zxp99688@vip.163.com

ABSTRACT

Purpose: To study the protecting effects of dexamethasone on thymus of rats with severe acute pancreatitis (SAP).

Methods: The SAP rats were randomly assigned to the model group and dexamethasone-treated group, the other normal healthy rats were assigned to the sham operation group. The rat survival, thymus pathological changes, apoptotic index, as well as expression levels of NF-kappaB, P-selectin, Bax, Bcl-2, and Caspase-3 protein of all groups were observed, respectively, at 3 hours, 6 hours, and 12 hours. The contents of amylase and endotoxin in plasma as well as the contents of TNF-alpha, PLA2, and NO in serum were determined.

Results: There was no marked difference between the model group and treated group in survival. The contents of different indexes in blood of treated group were lower than those of the model group to various degrees at different time points. The thymus pathological score was lower in treated group than in model group at 12 hours. The treated group in Caspase-3 protein expression of thymus significantly exceeded the model group at 12 hours. The apoptotic index was significantly higher in treated group than in model group.

Conclusion: Dexamethasone has protecting effects on thymus of SAP rats.

Show MeSH
Related in: MedlinePlus