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Ozonation of human blood induces a remarkable upregulation of heme oxygenase-1 and heat stress protein-70.

Bocci V, Aldinucci C, Mosci F, Carraro F, Valacchi G - Mediators Inflamm. (2007)

Bottom Line: The results show that production of both NO and HO-1 is fairly dose dependent but is particularly elevated using human plasma after transient exposure to a medium ozone concentration.At this concentration, there is also induction of Hsp-70.The results clarify another positive effect achievable by the use of ozone therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, University of Siena, 53100 Siena, Italy. bocci@unisi.it

ABSTRACT
Heme oxygenase-I (HO-1) has emerged as one of the most protective enzymes and its pleiotropic activities have been demonstrated in a variety of human pathologies. Unpublished observations have shown that HO-1 is induced after the infusion of ozonated blood into the respective donors, and many other experimental observations have demonstrated the efficacy of oxidizing agents. It appeared worthwhile to evaluate whether we could better define the activity of potential inducers such as hydrogen peroxide and ozonated human plasma. Human vascular endothelial cells at confluence were challenged with different concentrations of these inducers and the simultaneous production of nitric oxide (NO); and HO-1 was measured by either measuring nitrite, or bilirubin formation, or/and the immune reactivity of the protein by Western blot using a rabbit antihuman HO-1 and Hsp-70. The results show that production of both NO and HO-1 is fairly dose dependent but is particularly elevated using human plasma after transient exposure to a medium ozone concentration. At this concentration, there is also induction of Hsp-70. The results clarify another positive effect achievable by the use of ozone therapy.

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Related in: MedlinePlus

Ozonated human plasma, particularly at the medium ozone concentration of 40 μg/mL per mL of blood activates both HO-1 and Hsp-70 in human endothelial cells. The signals of both protein levels were determined by densitometric analysis of the scanned images. Data are expressed as arbitrary units. One representative Western blot of a typical experiment is shown in the top panel.
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fig2: Ozonated human plasma, particularly at the medium ozone concentration of 40 μg/mL per mL of blood activates both HO-1 and Hsp-70 in human endothelial cells. The signals of both protein levels were determined by densitometric analysis of the scanned images. Data are expressed as arbitrary units. One representative Western blot of a typical experiment is shown in the top panel.

Mentions: Both H2O2 and ozonated human plasma induce anincreased production of NO and bilirubin in a dose-dependent fashion up to noncytotoxicdoses. This trend has been confirmed in analyzing, by Western blot, some of thesamples; and in Figure 2, we can note that a medium-level plasma ozonation (40 μg/mL) is the most effective in inducing both HO-1 and Hsp-70. Interestingly,either the low or the high doses are less effective. 4-hydroxynonenal wasinhibitory probably because it was cytotoxic at the concentration of 10 μM.


Ozonation of human blood induces a remarkable upregulation of heme oxygenase-1 and heat stress protein-70.

Bocci V, Aldinucci C, Mosci F, Carraro F, Valacchi G - Mediators Inflamm. (2007)

Ozonated human plasma, particularly at the medium ozone concentration of 40 μg/mL per mL of blood activates both HO-1 and Hsp-70 in human endothelial cells. The signals of both protein levels were determined by densitometric analysis of the scanned images. Data are expressed as arbitrary units. One representative Western blot of a typical experiment is shown in the top panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2233812&req=5

fig2: Ozonated human plasma, particularly at the medium ozone concentration of 40 μg/mL per mL of blood activates both HO-1 and Hsp-70 in human endothelial cells. The signals of both protein levels were determined by densitometric analysis of the scanned images. Data are expressed as arbitrary units. One representative Western blot of a typical experiment is shown in the top panel.
Mentions: Both H2O2 and ozonated human plasma induce anincreased production of NO and bilirubin in a dose-dependent fashion up to noncytotoxicdoses. This trend has been confirmed in analyzing, by Western blot, some of thesamples; and in Figure 2, we can note that a medium-level plasma ozonation (40 μg/mL) is the most effective in inducing both HO-1 and Hsp-70. Interestingly,either the low or the high doses are less effective. 4-hydroxynonenal wasinhibitory probably because it was cytotoxic at the concentration of 10 μM.

Bottom Line: The results show that production of both NO and HO-1 is fairly dose dependent but is particularly elevated using human plasma after transient exposure to a medium ozone concentration.At this concentration, there is also induction of Hsp-70.The results clarify another positive effect achievable by the use of ozone therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, University of Siena, 53100 Siena, Italy. bocci@unisi.it

ABSTRACT
Heme oxygenase-I (HO-1) has emerged as one of the most protective enzymes and its pleiotropic activities have been demonstrated in a variety of human pathologies. Unpublished observations have shown that HO-1 is induced after the infusion of ozonated blood into the respective donors, and many other experimental observations have demonstrated the efficacy of oxidizing agents. It appeared worthwhile to evaluate whether we could better define the activity of potential inducers such as hydrogen peroxide and ozonated human plasma. Human vascular endothelial cells at confluence were challenged with different concentrations of these inducers and the simultaneous production of nitric oxide (NO); and HO-1 was measured by either measuring nitrite, or bilirubin formation, or/and the immune reactivity of the protein by Western blot using a rabbit antihuman HO-1 and Hsp-70. The results show that production of both NO and HO-1 is fairly dose dependent but is particularly elevated using human plasma after transient exposure to a medium ozone concentration. At this concentration, there is also induction of Hsp-70. The results clarify another positive effect achievable by the use of ozone therapy.

Show MeSH
Related in: MedlinePlus