Limits...
Human papillomavirus in endometrial adenocarcinomas: infectious agent or a mere "passenger"?

Giatromanolaki A, Sivridis E, Papazoglou D, Koukourakis MI, Maltezos E - Infect Dis Obstet Gynecol (2007)

Bottom Line: The results were related to histopathological features of the tumour, and the patients' age, and prognosis.None of the positive cases in the series, whether hyperplastic or neoplastic, demonstrated cytological evidence of HPV infection.There was no relation between HPV-positive cases and squamous differentiation, depth of myometrial invasion, lymphatic involvement, lymphocytic response, patients' age, or prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Democritus University of Thrace Medical School, 68100 Alexandroupolis, Greece. targ@her.forthmet.gr

ABSTRACT

Aims: To investigate the possible association of human papillomavirus (HPV) with endometrial hyperplasias and neoplasia. Does HPV play any role in the initiation or prognosis of endometrial adenocarcinomas?

Methods: Twenty-five endometrial adenocarcinomas of the endometrioid cell type, with and without squamous differentiation, and twenty-four endometrial hyperplasias of various forms (simple, complex, and atypical) were analyzed for the presence of type 16 and 18 HPV by the polymerase chain reaction (PCR). The results were related to histopathological features of the tumour, and the patients' age, and prognosis.

Results: Six of 25 endometrial adenocarcinomas were HPV 16-positive (24%), and 5 of 25 (20%) were HPV 18-positive. Simple endometrial hyperplasias was associated somewhat more commonly with HPV 16 and 18 (2/8 and 1/8 cases, resp.) than hyperplasias progressing to endometrial adenocarcinomas, namely, atypical endometrial hyperplasia (1/8 and 0/8 cases, resp.). None of the positive cases in the series, whether hyperplastic or neoplastic, demonstrated cytological evidence of HPV infection. There was no relation between HPV-positive cases and squamous differentiation, depth of myometrial invasion, lymphatic involvement, lymphocytic response, patients' age, or prognosis.

Conclusion: It appears that the presence of HPV in the endometrium, as detected by PCR, does not play any role in the initiation or prognosis of endometrial adenocarcinoma.

Show MeSH

Related in: MedlinePlus

Agarose gel analysis of endometrial adenocarcinomas by PCRusing HPV 16 type-specific primers. Line 1 (marker 100 bps), lines 2, 3, 6(positive samples), lines 4, 5 (negative samples), line 7 (negative control), andline 8 (positive control).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2233766&req=5

fig1: Agarose gel analysis of endometrial adenocarcinomas by PCRusing HPV 16 type-specific primers. Line 1 (marker 100 bps), lines 2, 3, 6(positive samples), lines 4, 5 (negative samples), line 7 (negative control), andline 8 (positive control).

Mentions: Detectionof HPV 16/18 DNA sequences in primary endometrial adenocarcinomas by PCRamplification products in representative specimens are shown in Figures 1 and 2, respectively.


Human papillomavirus in endometrial adenocarcinomas: infectious agent or a mere "passenger"?

Giatromanolaki A, Sivridis E, Papazoglou D, Koukourakis MI, Maltezos E - Infect Dis Obstet Gynecol (2007)

Agarose gel analysis of endometrial adenocarcinomas by PCRusing HPV 16 type-specific primers. Line 1 (marker 100 bps), lines 2, 3, 6(positive samples), lines 4, 5 (negative samples), line 7 (negative control), andline 8 (positive control).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2233766&req=5

fig1: Agarose gel analysis of endometrial adenocarcinomas by PCRusing HPV 16 type-specific primers. Line 1 (marker 100 bps), lines 2, 3, 6(positive samples), lines 4, 5 (negative samples), line 7 (negative control), andline 8 (positive control).
Mentions: Detectionof HPV 16/18 DNA sequences in primary endometrial adenocarcinomas by PCRamplification products in representative specimens are shown in Figures 1 and 2, respectively.

Bottom Line: The results were related to histopathological features of the tumour, and the patients' age, and prognosis.None of the positive cases in the series, whether hyperplastic or neoplastic, demonstrated cytological evidence of HPV infection.There was no relation between HPV-positive cases and squamous differentiation, depth of myometrial invasion, lymphatic involvement, lymphocytic response, patients' age, or prognosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Democritus University of Thrace Medical School, 68100 Alexandroupolis, Greece. targ@her.forthmet.gr

ABSTRACT

Aims: To investigate the possible association of human papillomavirus (HPV) with endometrial hyperplasias and neoplasia. Does HPV play any role in the initiation or prognosis of endometrial adenocarcinomas?

Methods: Twenty-five endometrial adenocarcinomas of the endometrioid cell type, with and without squamous differentiation, and twenty-four endometrial hyperplasias of various forms (simple, complex, and atypical) were analyzed for the presence of type 16 and 18 HPV by the polymerase chain reaction (PCR). The results were related to histopathological features of the tumour, and the patients' age, and prognosis.

Results: Six of 25 endometrial adenocarcinomas were HPV 16-positive (24%), and 5 of 25 (20%) were HPV 18-positive. Simple endometrial hyperplasias was associated somewhat more commonly with HPV 16 and 18 (2/8 and 1/8 cases, resp.) than hyperplasias progressing to endometrial adenocarcinomas, namely, atypical endometrial hyperplasia (1/8 and 0/8 cases, resp.). None of the positive cases in the series, whether hyperplastic or neoplastic, demonstrated cytological evidence of HPV infection. There was no relation between HPV-positive cases and squamous differentiation, depth of myometrial invasion, lymphatic involvement, lymphocytic response, patients' age, or prognosis.

Conclusion: It appears that the presence of HPV in the endometrium, as detected by PCR, does not play any role in the initiation or prognosis of endometrial adenocarcinoma.

Show MeSH
Related in: MedlinePlus