Limits...
Botulinum toxin in gastric submucosa reduces stimulated HCl production in rats.

Runfola M, Rossi S, Panunzi S, Spada PL, Gui D - BMC Gastroenterol (2003)

Bottom Line: HCl production after pyloric ligation was found to be significantly lower in botulinum toxin-treated rats (657 +/- 90.25 micromol HCl vs. 1247 +/- 152.Botulinum toxin-treated rats also showed significantly lower food intake and weight gain.Reduction of growth and food intake may reflect both impaired digestion and decreased gastric motility.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Surgery, Catholic University, Rome, Italy. runfola@tiscali.it

ABSTRACT

Background: Botulinum toxin blocks acetylcholine release from nerve endings and acts as a long term, reversible inhibitor of muscle contraction as well as of salivary, sweat gland, adrenal and prostatic secretions. The aim of the present study is to investigate whether gastric submucosal injection of botulinum toxin type A reduces stimulated gastric production of HCl.

Methods: Sixty-four rats were randomized in two groups and laparotomized. One group was treated with botulinum toxin-A 10 U by multiple submucosal gastric injections, while the second group was injected with saline. Two weeks later, acid secretion was stimulated by pyloric ligation and acid output was measured. Body weight, food and water intake were also recorded daily.

Results: HCl production after pyloric ligation was found to be significantly lower in botulinum toxin-treated rats (657 +/- 90.25 micromol HCl vs. 1247 +/- 152. P = 0.0017). Botulinum toxin-treated rats also showed significantly lower food intake and weight gain.

Conclusions: Botulinum toxin type A reduces stimulated gastric acidity. This is likely due either to inhibition of the cholinergic stimulation of gastric parietal cells, or to an action on the myenteric nervous plexuses. Reduction of growth and food intake may reflect both impaired digestion and decreased gastric motility.

Show MeSH

Related in: MedlinePlus

Time course of mean daily food intake in BTX and SALINE groups. Only survivors at day 14 were considered.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC222954&req=5

Figure 2: Time course of mean daily food intake in BTX and SALINE groups. Only survivors at day 14 were considered.

Mentions: Rate of body weight loss after surgery was faster, nadir time was later and rate of after-nadir weight gain was slower in BTX treated than in control animals (all differences highly significant). Even if rats were not significantly differing in body weight at baseline, the three effects above translate into a depressed weight recuperation of BTX rats after surgery. The average time courses of body weight for both groups are shown in Figure 1. The difference in the time course of body weight was paralleled by a significant difference in food and water consumption rates, Btx-A-treated rats eating and drinking less than control rats. Time courses of food and water intake for both groups are shown in Figures 2 and 3.


Botulinum toxin in gastric submucosa reduces stimulated HCl production in rats.

Runfola M, Rossi S, Panunzi S, Spada PL, Gui D - BMC Gastroenterol (2003)

Time course of mean daily food intake in BTX and SALINE groups. Only survivors at day 14 were considered.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC222954&req=5

Figure 2: Time course of mean daily food intake in BTX and SALINE groups. Only survivors at day 14 were considered.
Mentions: Rate of body weight loss after surgery was faster, nadir time was later and rate of after-nadir weight gain was slower in BTX treated than in control animals (all differences highly significant). Even if rats were not significantly differing in body weight at baseline, the three effects above translate into a depressed weight recuperation of BTX rats after surgery. The average time courses of body weight for both groups are shown in Figure 1. The difference in the time course of body weight was paralleled by a significant difference in food and water consumption rates, Btx-A-treated rats eating and drinking less than control rats. Time courses of food and water intake for both groups are shown in Figures 2 and 3.

Bottom Line: HCl production after pyloric ligation was found to be significantly lower in botulinum toxin-treated rats (657 +/- 90.25 micromol HCl vs. 1247 +/- 152.Botulinum toxin-treated rats also showed significantly lower food intake and weight gain.Reduction of growth and food intake may reflect both impaired digestion and decreased gastric motility.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Surgery, Catholic University, Rome, Italy. runfola@tiscali.it

ABSTRACT

Background: Botulinum toxin blocks acetylcholine release from nerve endings and acts as a long term, reversible inhibitor of muscle contraction as well as of salivary, sweat gland, adrenal and prostatic secretions. The aim of the present study is to investigate whether gastric submucosal injection of botulinum toxin type A reduces stimulated gastric production of HCl.

Methods: Sixty-four rats were randomized in two groups and laparotomized. One group was treated with botulinum toxin-A 10 U by multiple submucosal gastric injections, while the second group was injected with saline. Two weeks later, acid secretion was stimulated by pyloric ligation and acid output was measured. Body weight, food and water intake were also recorded daily.

Results: HCl production after pyloric ligation was found to be significantly lower in botulinum toxin-treated rats (657 +/- 90.25 micromol HCl vs. 1247 +/- 152. P = 0.0017). Botulinum toxin-treated rats also showed significantly lower food intake and weight gain.

Conclusions: Botulinum toxin type A reduces stimulated gastric acidity. This is likely due either to inhibition of the cholinergic stimulation of gastric parietal cells, or to an action on the myenteric nervous plexuses. Reduction of growth and food intake may reflect both impaired digestion and decreased gastric motility.

Show MeSH
Related in: MedlinePlus