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Prevalence of mutations associated with antimalarial drugs in Plasmodium falciparum isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran.

Zakeri S, Afsharpad M, Raeisi A, Djadid ND - Malar. J. (2007)

Bottom Line: The prevalence of pfdhps 437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the pfdhfr 108N, 59R with pfdhps 437G mutations.The frequency of allele pfcrt 76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried pfmdr1 86Y allele.Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was T76/N86/N51R59N108/A437 among both study areas.

View Article: PubMed Central - HTML - PubMed

Affiliation: Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Institut Pasteur of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran. zakeris@yahoo.com

ABSTRACT

Background: This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran.

Methods: The prevalence of pfcrt K76T, pfmdr1 N86Y, pfdhfr N51I, C59R, S108N/T and I164L and codons S436F/A, A437G, K540E, A581E, and A613S/T in pfdhps genes were genotyped by PCR/RFLP methods in 206 Plasmodium falciparum isolates from Chabahar and Sarbaz districts in Sistan and Baluchistan province, Iran, during 2003-2005.

Results: All P. falciparum isolates carried the 108N, while 98.5% parasite isolates carried the 59R mutation. 98.5% of patients carried both 108N and 59R. The prevalence of pfdhps 437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the pfdhfr 108N, 59R with pfdhps 437G mutations. The frequency of allele pfcrt 76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried pfmdr1 86Y allele. Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was T76/N86/N51R59N108/A437 among both study areas.

Conclusion: Finding the fixed level of CQ resistance polymorphisms (pfcrt 76T) suggests that CQ must be withdrawn from the current treatment strategy in Iran, while SP may remain the treatment of choice for uncomplicated malaria.

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Related in: MedlinePlus

Frequency distribution of the combination pfcrt/pfmdr1/pfdhfr/pfdhps haplotypes obtained from 206 isolates collected in Sistan and Baluchistan of Iran. The haplotype T76/N86/N51R59N108/A437 was the most prevalent among Chabahar (48%) and Sarbaz (43%) P. falciparum isolates. Mutated amino acids are boldfaced.
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Figure 2: Frequency distribution of the combination pfcrt/pfmdr1/pfdhfr/pfdhps haplotypes obtained from 206 isolates collected in Sistan and Baluchistan of Iran. The haplotype T76/N86/N51R59N108/A437 was the most prevalent among Chabahar (48%) and Sarbaz (43%) P. falciparum isolates. Mutated amino acids are boldfaced.

Mentions: Combination of pfcrt, pfmdr1, pfdhfr and pfdhps haplotypes among all 206 samples in this study demonstrated 8 distinct haplotypes (Figure 2). The two most prevalent haplotypes among samples were T76/N86/N51R59N108/A437 (47%) and T76/Y86/N51R59N108/A437 (28.6%). In addition, the majority of isolates from Chabahar (48%) and Sarbaz (43%) were belonging to haplotype T76/N86/N51R59N108/A437 (Figure 2). pfcrt 76T and pfmdr1 86Y with the pfdhfr 59R, 108N and pfdhps 437G mutations was detected in 10.7% isolates from Chabahar (n = 13) and Sarbaz (n = 9) districts (Table 2).


Prevalence of mutations associated with antimalarial drugs in Plasmodium falciparum isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran.

Zakeri S, Afsharpad M, Raeisi A, Djadid ND - Malar. J. (2007)

Frequency distribution of the combination pfcrt/pfmdr1/pfdhfr/pfdhps haplotypes obtained from 206 isolates collected in Sistan and Baluchistan of Iran. The haplotype T76/N86/N51R59N108/A437 was the most prevalent among Chabahar (48%) and Sarbaz (43%) P. falciparum isolates. Mutated amino acids are boldfaced.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2214731&req=5

Figure 2: Frequency distribution of the combination pfcrt/pfmdr1/pfdhfr/pfdhps haplotypes obtained from 206 isolates collected in Sistan and Baluchistan of Iran. The haplotype T76/N86/N51R59N108/A437 was the most prevalent among Chabahar (48%) and Sarbaz (43%) P. falciparum isolates. Mutated amino acids are boldfaced.
Mentions: Combination of pfcrt, pfmdr1, pfdhfr and pfdhps haplotypes among all 206 samples in this study demonstrated 8 distinct haplotypes (Figure 2). The two most prevalent haplotypes among samples were T76/N86/N51R59N108/A437 (47%) and T76/Y86/N51R59N108/A437 (28.6%). In addition, the majority of isolates from Chabahar (48%) and Sarbaz (43%) were belonging to haplotype T76/N86/N51R59N108/A437 (Figure 2). pfcrt 76T and pfmdr1 86Y with the pfdhfr 59R, 108N and pfdhps 437G mutations was detected in 10.7% isolates from Chabahar (n = 13) and Sarbaz (n = 9) districts (Table 2).

Bottom Line: The prevalence of pfdhps 437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the pfdhfr 108N, 59R with pfdhps 437G mutations.The frequency of allele pfcrt 76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried pfmdr1 86Y allele.Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was T76/N86/N51R59N108/A437 among both study areas.

View Article: PubMed Central - HTML - PubMed

Affiliation: Malaria and Vector Research Group (MVRG), Biotechnology Research Center, Institut Pasteur of Iran, Pasteur Avenue, PO BOX 1316943551, Tehran, Iran. zakeris@yahoo.com

ABSTRACT

Background: This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran.

Methods: The prevalence of pfcrt K76T, pfmdr1 N86Y, pfdhfr N51I, C59R, S108N/T and I164L and codons S436F/A, A437G, K540E, A581E, and A613S/T in pfdhps genes were genotyped by PCR/RFLP methods in 206 Plasmodium falciparum isolates from Chabahar and Sarbaz districts in Sistan and Baluchistan province, Iran, during 2003-2005.

Results: All P. falciparum isolates carried the 108N, while 98.5% parasite isolates carried the 59R mutation. 98.5% of patients carried both 108N and 59R. The prevalence of pfdhps 437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the pfdhfr 108N, 59R with pfdhps 437G mutations. The frequency of allele pfcrt 76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried pfmdr1 86Y allele. Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was T76/N86/N51R59N108/A437 among both study areas.

Conclusion: Finding the fixed level of CQ resistance polymorphisms (pfcrt 76T) suggests that CQ must be withdrawn from the current treatment strategy in Iran, while SP may remain the treatment of choice for uncomplicated malaria.

Show MeSH
Related in: MedlinePlus