Limits...
Activation of natural killer cells and dendritic cells upon recognition of a novel CD99-like ligand by paired immunoglobulin-like type 2 receptor.

Shiratori I, Ogasawara K, Saito T, Lanier LL, Arase H - J. Exp. Med. (2004)

Bottom Line: Transcripts of PILR ligand are present in many tissues, including some T cell lines.Cells expressing the PILR ligand specifically activated NK cells and dendritic cells that express the activating PILRbeta.Our findings reveal a new regulatory mechanism of innate immunity by PILR and its CD99-like ligand.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuoku, 260-8670, Japan.

ABSTRACT
Paired receptors that consist of highly related activating and inhibitory receptors are widely involved in the regulation of the immune system. Here, we report a mouse orthologue of the human activating paired immunoglobulin-like type 2 receptor (PILR) beta, which was cloned from a cDNA library of natural killer (NK) cells based on its ability to associate with the DAP12 signaling adaptor protein. The activating PILRbeta was expressed not only on NK cells but also on dendritic cells and macrophages. Furthermore, we have identified a novel CD99-like molecule as a ligand for the activating PILRbeta and inhibitory PILRalpha receptors. Transcripts of PILR ligand are present in many tissues, including some T cell lines. Cells expressing the PILR ligand specifically activated NK cells and dendritic cells that express the activating PILRbeta. Our findings reveal a new regulatory mechanism of innate immunity by PILR and its CD99-like ligand.

Show MeSH
Transcription of PILRα and PILRβ in various tissues and purified populations. Transcription of PILRα and PILRβ in various tissues (A) and purified populations (B) was analyzed by real-time quantitative RT-PCR. Transcription of β-actin was used as a standard, and relative expression levels of PILRα and PILRβ compared with amounts in the spleen are shown. BM-DC, bone marrow–derived dendritic cells. S. intestine, small intestine.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2211832&req=5

fig3: Transcription of PILRα and PILRβ in various tissues and purified populations. Transcription of PILRα and PILRβ in various tissues (A) and purified populations (B) was analyzed by real-time quantitative RT-PCR. Transcription of β-actin was used as a standard, and relative expression levels of PILRα and PILRβ compared with amounts in the spleen are shown. BM-DC, bone marrow–derived dendritic cells. S. intestine, small intestine.

Mentions: We analyzed the expression of PILRα and PILRβ transcripts by real-time quantitative PCR. Expressions of PILRα and PILRβ were relatively high in lung, liver, and spleen (Fig. 3 A). There was no significant difference in the tissue distribution between PILRα and PILRβ when analyzing their expression in total organs. We analyzed the expression of PILRβ in various purified populations (Fig. 3 B). Transcripts of PILRβ were detected not only in NK cells but also in BM-DCs, peritoneal macrophages, and granulocytes. Because DAP12 that is required for signaling of PILRβ is widely distributed not only in NK cells but also in cells of the myeloid lineage, including macrophages, granulocytes, and DCs, PILRβ may play a role in the activation of these populations. On the other hand, macrophages and granulocytes also expressed the inhibitory PILRα. In contrast, NK cells and BM-DCs expressed a very low amount of PILRα transcripts. Therefore, the expression pattern of PILRα and PILRβ seems to vary depending on cell types and their activation status.


Activation of natural killer cells and dendritic cells upon recognition of a novel CD99-like ligand by paired immunoglobulin-like type 2 receptor.

Shiratori I, Ogasawara K, Saito T, Lanier LL, Arase H - J. Exp. Med. (2004)

Transcription of PILRα and PILRβ in various tissues and purified populations. Transcription of PILRα and PILRβ in various tissues (A) and purified populations (B) was analyzed by real-time quantitative RT-PCR. Transcription of β-actin was used as a standard, and relative expression levels of PILRα and PILRβ compared with amounts in the spleen are shown. BM-DC, bone marrow–derived dendritic cells. S. intestine, small intestine.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211832&req=5

fig3: Transcription of PILRα and PILRβ in various tissues and purified populations. Transcription of PILRα and PILRβ in various tissues (A) and purified populations (B) was analyzed by real-time quantitative RT-PCR. Transcription of β-actin was used as a standard, and relative expression levels of PILRα and PILRβ compared with amounts in the spleen are shown. BM-DC, bone marrow–derived dendritic cells. S. intestine, small intestine.
Mentions: We analyzed the expression of PILRα and PILRβ transcripts by real-time quantitative PCR. Expressions of PILRα and PILRβ were relatively high in lung, liver, and spleen (Fig. 3 A). There was no significant difference in the tissue distribution between PILRα and PILRβ when analyzing their expression in total organs. We analyzed the expression of PILRβ in various purified populations (Fig. 3 B). Transcripts of PILRβ were detected not only in NK cells but also in BM-DCs, peritoneal macrophages, and granulocytes. Because DAP12 that is required for signaling of PILRβ is widely distributed not only in NK cells but also in cells of the myeloid lineage, including macrophages, granulocytes, and DCs, PILRβ may play a role in the activation of these populations. On the other hand, macrophages and granulocytes also expressed the inhibitory PILRα. In contrast, NK cells and BM-DCs expressed a very low amount of PILRα transcripts. Therefore, the expression pattern of PILRα and PILRβ seems to vary depending on cell types and their activation status.

Bottom Line: Transcripts of PILR ligand are present in many tissues, including some T cell lines.Cells expressing the PILR ligand specifically activated NK cells and dendritic cells that express the activating PILRbeta.Our findings reveal a new regulatory mechanism of innate immunity by PILR and its CD99-like ligand.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuoku, 260-8670, Japan.

ABSTRACT
Paired receptors that consist of highly related activating and inhibitory receptors are widely involved in the regulation of the immune system. Here, we report a mouse orthologue of the human activating paired immunoglobulin-like type 2 receptor (PILR) beta, which was cloned from a cDNA library of natural killer (NK) cells based on its ability to associate with the DAP12 signaling adaptor protein. The activating PILRbeta was expressed not only on NK cells but also on dendritic cells and macrophages. Furthermore, we have identified a novel CD99-like molecule as a ligand for the activating PILRbeta and inhibitory PILRalpha receptors. Transcripts of PILR ligand are present in many tissues, including some T cell lines. Cells expressing the PILR ligand specifically activated NK cells and dendritic cells that express the activating PILRbeta. Our findings reveal a new regulatory mechanism of innate immunity by PILR and its CD99-like ligand.

Show MeSH