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Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array.

Koo SH, Ong TC, Chong KT, Lee CG, Chew FT, Lee EJ - Biol Proced Online (2007)

Bottom Line: Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management.The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping.It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

View Article: PubMed Central - PubMed

Affiliation: Singapore Department of Pharmacology, National University of Singapore.

ABSTRACT
We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

No MeSH data available.


Related in: MedlinePlus

Fig. 3Graphical representation of allelic ratio (A) Subject 34 and (B) Subject 12.
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f3: Fig. 3Graphical representation of allelic ratio (A) Subject 34 and (B) Subject 12.

Mentions: Figure 3 depicts the graphical representation of allelic ratio for two of the study subjects. All the 352 genotypes (22 SNPs x 8 subjects x 2 i.e. duplicates) determined by the Bioplex suspension array were 100% concordant with results obtained using the sequencing approach.


Multiplexed genotyping of ABC transporter polymorphisms with the Bioplex suspension array.

Koo SH, Ong TC, Chong KT, Lee CG, Chew FT, Lee EJ - Biol Proced Online (2007)

Fig. 3Graphical representation of allelic ratio (A) Subject 34 and (B) Subject 12.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211573&req=5

f3: Fig. 3Graphical representation of allelic ratio (A) Subject 34 and (B) Subject 12.
Mentions: Figure 3 depicts the graphical representation of allelic ratio for two of the study subjects. All the 352 genotypes (22 SNPs x 8 subjects x 2 i.e. duplicates) determined by the Bioplex suspension array were 100% concordant with results obtained using the sequencing approach.

Bottom Line: Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management.The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping.It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

View Article: PubMed Central - PubMed

Affiliation: Singapore Department of Pharmacology, National University of Singapore.

ABSTRACT
We have developed and validated a consolidated bead-based genotyping platform, the Bioplex suspension array for simultaneous detection of multiple single nucleotide polymorphisms (SNPs) of the ATP-binding cassette transporters. Genetic polymorphisms have been known to influence therapeutic response and risk of disease pathologies. Genetic screening for therapeutic and diagnostic applications thus holds great promise in clinical management. The allele-specific primer extension (ASPE) reaction was used to assay 22 multiplexed SNPs for eight subjects. Comparison of the microsphere-based ASPE assay results to sequencing results showed complete concordance in genotype assignments. The Bioplex suspension array thus proves to be a reliable, cost-effective and high-throughput technological platform for genotyping. It can be easily adapted to customized SNP panels for specific applications involving large-scale mutation screening of clinically relevant markers.

No MeSH data available.


Related in: MedlinePlus