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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


Related in: MedlinePlus

The gene expression spectra of a high-yield cotton compared to a common cotton variety.
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Related In: Results  -  Collection


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fig8: The gene expression spectra of a high-yield cotton compared to a common cotton variety.

Mentions: The gene expression analysis is an important method to study the different gene functions of organisms, which is usually performed on high-density biochips with several tens of thousands of probes in a small 20 mm × 60 mm area, where dual fluorescent color labels with the dyesCy3 and Cy5 can be used to produce a gene expression spectrum. In order to analyze the gene expression of biochips, it is important for the scanning system to possess high scanning resolution, an excellent contrast, and signal-to-noise ratio, over a wide range of signal intensities. The newly developed confocal optical scanning system can be readily used for the gene expression analysis of biochips. The gene expression spectrum for a high-yield variety of cotton as shown in Figure 8 was obtained by using the developed confocal scanner.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The gene expression spectra of a high-yield cotton compared to a common cotton variety.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig8: The gene expression spectra of a high-yield cotton compared to a common cotton variety.
Mentions: The gene expression analysis is an important method to study the different gene functions of organisms, which is usually performed on high-density biochips with several tens of thousands of probes in a small 20 mm × 60 mm area, where dual fluorescent color labels with the dyesCy3 and Cy5 can be used to produce a gene expression spectrum. In order to analyze the gene expression of biochips, it is important for the scanning system to possess high scanning resolution, an excellent contrast, and signal-to-noise ratio, over a wide range of signal intensities. The newly developed confocal optical scanning system can be readily used for the gene expression analysis of biochips. The gene expression spectrum for a high-yield variety of cotton as shown in Figure 8 was obtained by using the developed confocal scanner.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


Related in: MedlinePlus