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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


The analysis features of the newly developed confocal scanner.
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Related In: Results  -  Collection


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fig7: The analysis features of the newly developed confocal scanner.

Mentions: When pairs of identical gradient signal biochips from Full Moon ( Full Moon BioSystems, Sunnyvale, Calif, USA) were labeled with either Cy3-tagged or Cy5-tagged probes, and dual color fluorescence was detected by using our newly developed confocal scanner and by another commercial confocal scanner (US popular brand S), the features of the scanning image analyses are shown in Figures 7(a)–7(d), where the right small spot array image is a local area magnifying view for left scanning images. Our newly developed confocal scanner shows a high scanning resolution, an excellent contrast, and a signal-to-noise ratio seen in Figures 7(a) and 7(c). The detection sensitivity of the new confocal scanner was further illustrated in Figure 7(e), where a Full Moon (Full Moon BioSystems, Sunnyvale, Calif, USA) normal molecular density biochip wasused and the unit of density is the molecule number per the square micron, and the signal is the relative intensity in the range of 0 to 65 535. In Figure 7(e), a molecule with a density of 0.071 fluors/m2 was detected with a signal of 547, where SNR (signal-to-noise ratio) is greater than 2, whichindicates that the new confocal scanner is with a sensitivity of fluorescence about 0.1 fluors/m2.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The analysis features of the newly developed confocal scanner.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig7: The analysis features of the newly developed confocal scanner.
Mentions: When pairs of identical gradient signal biochips from Full Moon ( Full Moon BioSystems, Sunnyvale, Calif, USA) were labeled with either Cy3-tagged or Cy5-tagged probes, and dual color fluorescence was detected by using our newly developed confocal scanner and by another commercial confocal scanner (US popular brand S), the features of the scanning image analyses are shown in Figures 7(a)–7(d), where the right small spot array image is a local area magnifying view for left scanning images. Our newly developed confocal scanner shows a high scanning resolution, an excellent contrast, and a signal-to-noise ratio seen in Figures 7(a) and 7(c). The detection sensitivity of the new confocal scanner was further illustrated in Figure 7(e), where a Full Moon (Full Moon BioSystems, Sunnyvale, Calif, USA) normal molecular density biochip wasused and the unit of density is the molecule number per the square micron, and the signal is the relative intensity in the range of 0 to 65 535. In Figure 7(e), a molecule with a density of 0.071 fluors/m2 was detected with a signal of 547, where SNR (signal-to-noise ratio) is greater than 2, whichindicates that the new confocal scanner is with a sensitivity of fluorescence about 0.1 fluors/m2.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.