Limits...
Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


The newly developed confocal optical scanning system.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2211516&req=5

fig6: The newly developed confocal optical scanning system.

Mentions: Applying the above optical design parameters, we constructed a new confocal optical scanning system which has been further developed into the advanced confocal scanner specially for biochip application, as illustrated inFigure 6, where the objective and magnifying lenses are designed in Figure 1, two filters for the dye Cy3 and the dye Cy5 are bought from Chroma Corporation, PMT (photomultiplier tube) is bought from Hamamatsu Corporation, laser 1 is a solid laser with wavelength 532 nm and power 25 mW, laser 2 is a semiconductor laser with wavelength 635 nm and power 25 mW, splitter is a dichroic mirror from Chroma Corporation, the mirror is machined into an elliptical mirror with a small hole 1mm in center, XY scanning platform is designed with 2m moving control precision. Pinhole is a small hole with diameter 20m, A/D electronic card is designed with precision 16 bit and frequency 1 MHz. Computer is chosen with PIII CPU or higher CPU speed, biochip is developed by CapitalBio Corporation. It is characterized by an objective with a large numeral aperture of , a long working distance of 3.0 mm, and a sensitivity of fluorescence detection of about 0.1 fluors/m2. Compared to other similar commercial scanners, it has a higher-resolution power and an excellent signal-to-noise ratio. In Figure 6, when the laser beam irradiates the biochip on the XY scanning platform from the laser 1 with wavelength 532 nm, or from the laser 2 with wavelength 635 nm, the fluorescence of biological sample on the biochip is induced and collected by PMT. After A/D (analogue/digital) transfer is complete, the fluorescence signal from the biochip is loaded into the computerfor digital image processing.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The newly developed confocal optical scanning system.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig6: The newly developed confocal optical scanning system.
Mentions: Applying the above optical design parameters, we constructed a new confocal optical scanning system which has been further developed into the advanced confocal scanner specially for biochip application, as illustrated inFigure 6, where the objective and magnifying lenses are designed in Figure 1, two filters for the dye Cy3 and the dye Cy5 are bought from Chroma Corporation, PMT (photomultiplier tube) is bought from Hamamatsu Corporation, laser 1 is a solid laser with wavelength 532 nm and power 25 mW, laser 2 is a semiconductor laser with wavelength 635 nm and power 25 mW, splitter is a dichroic mirror from Chroma Corporation, the mirror is machined into an elliptical mirror with a small hole 1mm in center, XY scanning platform is designed with 2m moving control precision. Pinhole is a small hole with diameter 20m, A/D electronic card is designed with precision 16 bit and frequency 1 MHz. Computer is chosen with PIII CPU or higher CPU speed, biochip is developed by CapitalBio Corporation. It is characterized by an objective with a large numeral aperture of , a long working distance of 3.0 mm, and a sensitivity of fluorescence detection of about 0.1 fluors/m2. Compared to other similar commercial scanners, it has a higher-resolution power and an excellent signal-to-noise ratio. In Figure 6, when the laser beam irradiates the biochip on the XY scanning platform from the laser 1 with wavelength 532 nm, or from the laser 2 with wavelength 635 nm, the fluorescence of biological sample on the biochip is induced and collected by PMT. After A/D (analogue/digital) transfer is complete, the fluorescence signal from the biochip is loaded into the computerfor digital image processing.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.