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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


The geometric and diffraction image analyses of the system illustrated in Figure 1.
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Related In: Results  -  Collection


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fig5: The geometric and diffraction image analyses of the system illustrated in Figure 1.

Mentions: Geometric image analysis is used to model extended sources, to analyze useful resolution, to representthe appearance of imaged objects, and to provide intuition as to image rotation. The diffraction image analysis accounts for the finite pass band and other diffraction-related effects of real optical systems based upon Fourier Optics. The diffraction image analysis of the system is shown in Figure 5. The geometric image analysis of the optical system is shown in Figure 5(a), while the diffraction image analysis of the optical system in the object area illuminated by the scanning spot is shown in Figure 5(b), both of which have a high efficiency of 100%. In Figure 5(a), the geometric image analysis shows a nice roundness of model extended sources, thereis good image rotation invariability of system. In Figure 5(b), the diffraction image analysis shows a nice uniformity and clear outline of the letter F as an object, there is good image quality of system to the object area illuminated.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The geometric and diffraction image analyses of the system illustrated in Figure 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig5: The geometric and diffraction image analyses of the system illustrated in Figure 1.
Mentions: Geometric image analysis is used to model extended sources, to analyze useful resolution, to representthe appearance of imaged objects, and to provide intuition as to image rotation. The diffraction image analysis accounts for the finite pass band and other diffraction-related effects of real optical systems based upon Fourier Optics. The diffraction image analysis of the system is shown in Figure 5. The geometric image analysis of the optical system is shown in Figure 5(a), while the diffraction image analysis of the optical system in the object area illuminated by the scanning spot is shown in Figure 5(b), both of which have a high efficiency of 100%. In Figure 5(a), the geometric image analysis shows a nice roundness of model extended sources, thereis good image rotation invariability of system. In Figure 5(b), the diffraction image analysis shows a nice uniformity and clear outline of the letter F as an object, there is good image quality of system to the object area illuminated.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.