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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


The optical path difference and the encircled energy diagram of the system.
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Related In: Results  -  Collection


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fig4: The optical path difference and the encircled energy diagram of the system.

Mentions: The optical path difference (OPD) is a scalar quantity and it is identical to those for ray aberration fans at the tangential and sagittal directions PX and PY, respectively. The data plotted in Figure 4(a) is the optical path difference of the system in Figure 1, which is the difference between the optical path length of the ray and the optical path length of the chief ray. The horizontal scale of graph is the normalized entrance pupil coordinate. The vertical axis scale of graph is one wave, while the OPD maximum of system in Figure 1 is smaller than 5 waves. In Figure 4(a), there is a small optical path difference among 3 wavelengths 570 nm, 620 nm, and 670 nm, but the maximum optical path difference is smaller than 3 waves, which is lower than a normal visible light imaging system of 5 waves and can be used for confocal scanning system very well.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The optical path difference and the encircled energy diagram of the system.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig4: The optical path difference and the encircled energy diagram of the system.
Mentions: The optical path difference (OPD) is a scalar quantity and it is identical to those for ray aberration fans at the tangential and sagittal directions PX and PY, respectively. The data plotted in Figure 4(a) is the optical path difference of the system in Figure 1, which is the difference between the optical path length of the ray and the optical path length of the chief ray. The horizontal scale of graph is the normalized entrance pupil coordinate. The vertical axis scale of graph is one wave, while the OPD maximum of system in Figure 1 is smaller than 5 waves. In Figure 4(a), there is a small optical path difference among 3 wavelengths 570 nm, 620 nm, and 670 nm, but the maximum optical path difference is smaller than 3 waves, which is lower than a normal visible light imaging system of 5 waves and can be used for confocal scanning system very well.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.