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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


Related in: MedlinePlus

The spot diagram of the scanning probe.
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fig3: The spot diagram of the scanning probe.

Mentions: In a confocal scanning system, the laser scanning spot probe determines the scanning resolution power. The smaller the laser scanning spot of optical system, the higher the scanning resolution power of the confocalsystem. The scanning spot diagrams are referenced to the real chief ray as shown in Figure 3. This option allows selection of two other reference points, the centroid and the middle. The centroid is defined by the distribution of the traced rays. The middle is defined so that the maximum ray errors are equal in both the x- and the y-directions. When a laser with a real beam diameter of about 1 mm is imaged by the objective shown above in Figure 1, then the spot diagram on the focal plane is as shown in Figure 3, where the scale is 0.4 m, the spot diameter on the focal plane is smaller than 0.4 m, which is an ideal scanning probe beam with a more high resolving power of m.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The spot diagram of the scanning probe.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig3: The spot diagram of the scanning probe.
Mentions: In a confocal scanning system, the laser scanning spot probe determines the scanning resolution power. The smaller the laser scanning spot of optical system, the higher the scanning resolution power of the confocalsystem. The scanning spot diagrams are referenced to the real chief ray as shown in Figure 3. This option allows selection of two other reference points, the centroid and the middle. The centroid is defined by the distribution of the traced rays. The middle is defined so that the maximum ray errors are equal in both the x- and the y-directions. When a laser with a real beam diameter of about 1 mm is imaged by the objective shown above in Figure 1, then the spot diagram on the focal plane is as shown in Figure 3, where the scale is 0.4 m, the spot diameter on the focal plane is smaller than 0.4 m, which is an ideal scanning probe beam with a more high resolving power of m.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


Related in: MedlinePlus