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Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.


The configuration of the objective collecting fluorescence.
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Related In: Results  -  Collection


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fig2: The configuration of the objective collecting fluorescence.

Mentions: The optical structure parameters of confocal scanning system were optimized in Figure 1, where there are an objective and a magnifying lenses, the objective consists of seven lenses, the magnifying lenses consists of five lenses, there is a parallel beam between the objective and magnifying lenses. The optical structure parameters of confocal scanning system are listed inTable 1. A configuration of the objective collecting fluorescence is shown in Figure 2, where the distance from the focal plane to the front surface of first lens of objective is , r is the radius of effective aperture of the objective, is the half of aperture angle. The emission fluorescence (Em-fluor) of molecule bound on biochips is ideally a spherical wave, and the fluorescence bound on the biochip collected by the objective as shown in Figure 2 is described approximately by the formula (2)I(Z)≈K02Z2×π×r2,where is a constant, is the distance from the center of source in the focal plane to the first surface of objective, r is the radius of effective aperture of the objective.


Development of a confocal optical system design for molecular imaging applications of biochip.

Huang G, Xu S, Zhu J, Deng C, Dong Z, Yang Y, Yang X, Wang X, Jin G - Int J Biomed Imaging (2007)

The configuration of the objective collecting fluorescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2211516&req=5

fig2: The configuration of the objective collecting fluorescence.
Mentions: The optical structure parameters of confocal scanning system were optimized in Figure 1, where there are an objective and a magnifying lenses, the objective consists of seven lenses, the magnifying lenses consists of five lenses, there is a parallel beam between the objective and magnifying lenses. The optical structure parameters of confocal scanning system are listed inTable 1. A configuration of the objective collecting fluorescence is shown in Figure 2, where the distance from the focal plane to the front surface of first lens of objective is , r is the radius of effective aperture of the objective, is the half of aperture angle. The emission fluorescence (Em-fluor) of molecule bound on biochips is ideally a spherical wave, and the fluorescence bound on the biochip collected by the objective as shown in Figure 2 is described approximately by the formula (2)I(Z)≈K02Z2×π×r2,where is a constant, is the distance from the center of source in the focal plane to the first surface of objective, r is the radius of effective aperture of the objective.

Bottom Line: A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used.This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners.Some applications of gene and protein imagings using the dual laser confocal scanner are described.

View Article: PubMed Central - PubMed

Affiliation: Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China.

ABSTRACT
A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0 mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.

No MeSH data available.