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Microbial sulfate reduction and metal attenuation in pH 4 acid mine water.

Church CD, Wilkin RT, Alpers CN, Rye RO, McCleskey RB - Geochem. Trans. (2007)

Bottom Line: Scanning electron microscope (SEM) analyses of sediment show 1.5-micrometer, spherical ZnS precipitates.Phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) analyses of Penn Mine sediment show a high biomass level with a moderately diverse community structure composed primarily of iron- and sulfate-reducing bacteria.DGGE coupled with sequence and phylogenetic analysis of 16S rDNA gene segments showed populations of Desulfosporosinus and Desulfitobacterium in Penn Mine sediment and laboratory cultures.

View Article: PubMed Central - HTML - PubMed

Affiliation: US Geological Survey, California Water Science Center, 4165 Spruance Road, San Diego, CA 92101, USA. clinton.church@ars.usda.gov

ABSTRACT
Sediments recovered from the flooded mine workings of the Penn Mine, a Cu-Zn mine abandoned since the early 1960s, were cultured for anaerobic bacteria over a range of pH (4.0 to 7.5). The molecular biology of sediments and cultures was studied to determine whether sulfate-reducing bacteria (SRB) were active in moderately acidic conditions present in the underground mine workings. Here we document multiple, independent analyses and show evidence that sulfate reduction and associated metal attenuation are occurring in the pH-4 mine environment. Water-chemistry analyses of the mine water reveal: (1) preferential complexation and precipitation by H2S of Cu and Cd, relative to Zn; (2) stable isotope ratios of 34S/32S and 18O/16O in dissolved SO4 that are 2-3 per thousand heavier in the mine water, relative to those in surface waters; (3) reduction/oxidation conditions and dissolved gas concentrations consistent with conditions to support anaerobic processes such as sulfate reduction. Scanning electron microscope (SEM) analyses of sediment show 1.5-micrometer, spherical ZnS precipitates. Phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) analyses of Penn Mine sediment show a high biomass level with a moderately diverse community structure composed primarily of iron- and sulfate-reducing bacteria. Cultures of sediment from the mine produced dissolved sulfide at pH values near 7 and near 4, forming precipitates of either iron sulfide or elemental sulfur. DGGE coupled with sequence and phylogenetic analysis of 16S rDNA gene segments showed populations of Desulfosporosinus and Desulfitobacterium in Penn Mine sediment and laboratory cultures.

No MeSH data available.


DGGE gel image of 16S rDNA fragments from microbial cultures derived from Penn Mine sediments with seven bands labeled (Table 4). PM01: water from culture experiments; PM02: low-pH cultures; PM03: near-neutral-pH cultures.
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Figure 6: DGGE gel image of 16S rDNA fragments from microbial cultures derived from Penn Mine sediments with seven bands labeled (Table 4). PM01: water from culture experiments; PM02: low-pH cultures; PM03: near-neutral-pH cultures.

Mentions: The DGGE profiles from the experimental cultures showed few prominent bands in each of the samples and few similarities between profiles (Figure 6). As expected from the observed trends in sulfide production shown in Figure 4, sulfur-reducing bacterial sequences were prominent in both samples. A sequence recovered from the water from experiment PM03 aligned with aerobic/facultatively aerobic bacterial sequences. The sequences recovered from the corresponding solid sample aligned with an aerobic bacterium, Bacillus. The results are summarized in Table 4 and discussed below.


Microbial sulfate reduction and metal attenuation in pH 4 acid mine water.

Church CD, Wilkin RT, Alpers CN, Rye RO, McCleskey RB - Geochem. Trans. (2007)

DGGE gel image of 16S rDNA fragments from microbial cultures derived from Penn Mine sediments with seven bands labeled (Table 4). PM01: water from culture experiments; PM02: low-pH cultures; PM03: near-neutral-pH cultures.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2211471&req=5

Figure 6: DGGE gel image of 16S rDNA fragments from microbial cultures derived from Penn Mine sediments with seven bands labeled (Table 4). PM01: water from culture experiments; PM02: low-pH cultures; PM03: near-neutral-pH cultures.
Mentions: The DGGE profiles from the experimental cultures showed few prominent bands in each of the samples and few similarities between profiles (Figure 6). As expected from the observed trends in sulfide production shown in Figure 4, sulfur-reducing bacterial sequences were prominent in both samples. A sequence recovered from the water from experiment PM03 aligned with aerobic/facultatively aerobic bacterial sequences. The sequences recovered from the corresponding solid sample aligned with an aerobic bacterium, Bacillus. The results are summarized in Table 4 and discussed below.

Bottom Line: Scanning electron microscope (SEM) analyses of sediment show 1.5-micrometer, spherical ZnS precipitates.Phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) analyses of Penn Mine sediment show a high biomass level with a moderately diverse community structure composed primarily of iron- and sulfate-reducing bacteria.DGGE coupled with sequence and phylogenetic analysis of 16S rDNA gene segments showed populations of Desulfosporosinus and Desulfitobacterium in Penn Mine sediment and laboratory cultures.

View Article: PubMed Central - HTML - PubMed

Affiliation: US Geological Survey, California Water Science Center, 4165 Spruance Road, San Diego, CA 92101, USA. clinton.church@ars.usda.gov

ABSTRACT
Sediments recovered from the flooded mine workings of the Penn Mine, a Cu-Zn mine abandoned since the early 1960s, were cultured for anaerobic bacteria over a range of pH (4.0 to 7.5). The molecular biology of sediments and cultures was studied to determine whether sulfate-reducing bacteria (SRB) were active in moderately acidic conditions present in the underground mine workings. Here we document multiple, independent analyses and show evidence that sulfate reduction and associated metal attenuation are occurring in the pH-4 mine environment. Water-chemistry analyses of the mine water reveal: (1) preferential complexation and precipitation by H2S of Cu and Cd, relative to Zn; (2) stable isotope ratios of 34S/32S and 18O/16O in dissolved SO4 that are 2-3 per thousand heavier in the mine water, relative to those in surface waters; (3) reduction/oxidation conditions and dissolved gas concentrations consistent with conditions to support anaerobic processes such as sulfate reduction. Scanning electron microscope (SEM) analyses of sediment show 1.5-micrometer, spherical ZnS precipitates. Phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) analyses of Penn Mine sediment show a high biomass level with a moderately diverse community structure composed primarily of iron- and sulfate-reducing bacteria. Cultures of sediment from the mine produced dissolved sulfide at pH values near 7 and near 4, forming precipitates of either iron sulfide or elemental sulfur. DGGE coupled with sequence and phylogenetic analysis of 16S rDNA gene segments showed populations of Desulfosporosinus and Desulfitobacterium in Penn Mine sediment and laboratory cultures.

No MeSH data available.