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Patterns of airway inflammation and MMP-12 expression in smokers and ex-smokers with COPD.

Babusyte A, Stravinskaite K, Jeroch J, Lötvall J, Sakalauskas R, Sitkauskiene B - Respir. Res. (2007)

Bottom Line: Smoking activates and recruits inflammatory cells and proteases to the airways.IS and BAL samples were obtained for differential and MMP-12+-macrophages count analysis.The amount of BAL neutrophils was higher in COPD smokers compared to healthy never-smokers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pulmonology, Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 4, LT-50009, Kaunas, Lithuania. agne.babusyte@gmail.com

ABSTRACT

Background: Smoking activates and recruits inflammatory cells and proteases to the airways. Matrix metalloproteinase (MMP)-12 may be a key mediator in smoke induced emphysema. However, the influence of smoking and its cessation on airway inflammation and MMP-12 expression during COPD is still unknown. We aimed to analyse airway inflammatory cell patterns in induced sputum (IS) and bronchoalveolar lavage (BAL) from COPD patients who are active smokers and who have ceased smoking >2 years ago.

Methods: 39 COPD outpatients - smokers (n = 22) and ex-smokers (n = 17) were studied. 8 'healthy' smokers and 11 healthy never-smokers were tested as the control groups. IS and BAL samples were obtained for differential and MMP-12+-macrophages count analysis.

Results: The number of IS neutrophils was higher in both COPD groups compared to both controls. The amount of BAL neutrophils was higher in COPD smokers compared to healthy never-smokers. The number of BAL MMP-12+-macrophages was higher in COPD smokers (1.6 +/- 0.3 x 106/ml) compared to COPD ex-smokers, 'healthy' smokers and healthy never-smokers (0.9 +/- 0.4, 0.4 +/- 0.2, 0.2 +/- 0.1 x 106/ml respectively, p < 0.05).

Conclusion: The lower amount of BAL neutrophils in COPD ex-smokers, compared to COPD smokers, suggests positive alterations in alveolar compartment after smoking cessation. Smoking and disease itself may stimulate MMP-12 expression in airway compartments (IS and BAL) from COPD patients.

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Related in: MedlinePlus

Differential cell counts in IS and BAL (106/ml). Differential cell composition in IS (A) and BAL (B) from COPD smokers, COPD ex-smokers, 'healthy' smokers and healthy never-smokers. Data are shown as mean ± SEM. *p < 0.05 compared to healthy never-smokers, #p < 0.05 compared to 'healthy' smokers.
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Figure 2: Differential cell counts in IS and BAL (106/ml). Differential cell composition in IS (A) and BAL (B) from COPD smokers, COPD ex-smokers, 'healthy' smokers and healthy never-smokers. Data are shown as mean ± SEM. *p < 0.05 compared to healthy never-smokers, #p < 0.05 compared to 'healthy' smokers.

Mentions: The total cell count of IS did not differ between all groups (Fig. 2A). The composition of inflammatory cells did not differ between COPD smokers and COPD ex-smokers. COPD groups showed a predominance of neutrophils, compared to both healthy subjects groups in percentages (Table 2). An absolute amount of these cells was higher in COPD smokers and COPD ex-smokers compared to healthy never-smokers, but not 'healthy' smokers (Fig. 2A).


Patterns of airway inflammation and MMP-12 expression in smokers and ex-smokers with COPD.

Babusyte A, Stravinskaite K, Jeroch J, Lötvall J, Sakalauskas R, Sitkauskiene B - Respir. Res. (2007)

Differential cell counts in IS and BAL (106/ml). Differential cell composition in IS (A) and BAL (B) from COPD smokers, COPD ex-smokers, 'healthy' smokers and healthy never-smokers. Data are shown as mean ± SEM. *p < 0.05 compared to healthy never-smokers, #p < 0.05 compared to 'healthy' smokers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2200652&req=5

Figure 2: Differential cell counts in IS and BAL (106/ml). Differential cell composition in IS (A) and BAL (B) from COPD smokers, COPD ex-smokers, 'healthy' smokers and healthy never-smokers. Data are shown as mean ± SEM. *p < 0.05 compared to healthy never-smokers, #p < 0.05 compared to 'healthy' smokers.
Mentions: The total cell count of IS did not differ between all groups (Fig. 2A). The composition of inflammatory cells did not differ between COPD smokers and COPD ex-smokers. COPD groups showed a predominance of neutrophils, compared to both healthy subjects groups in percentages (Table 2). An absolute amount of these cells was higher in COPD smokers and COPD ex-smokers compared to healthy never-smokers, but not 'healthy' smokers (Fig. 2A).

Bottom Line: Smoking activates and recruits inflammatory cells and proteases to the airways.IS and BAL samples were obtained for differential and MMP-12+-macrophages count analysis.The amount of BAL neutrophils was higher in COPD smokers compared to healthy never-smokers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Pulmonology, Institute for Biomedical Research, Kaunas University of Medicine, Eiveniu 4, LT-50009, Kaunas, Lithuania. agne.babusyte@gmail.com

ABSTRACT

Background: Smoking activates and recruits inflammatory cells and proteases to the airways. Matrix metalloproteinase (MMP)-12 may be a key mediator in smoke induced emphysema. However, the influence of smoking and its cessation on airway inflammation and MMP-12 expression during COPD is still unknown. We aimed to analyse airway inflammatory cell patterns in induced sputum (IS) and bronchoalveolar lavage (BAL) from COPD patients who are active smokers and who have ceased smoking >2 years ago.

Methods: 39 COPD outpatients - smokers (n = 22) and ex-smokers (n = 17) were studied. 8 'healthy' smokers and 11 healthy never-smokers were tested as the control groups. IS and BAL samples were obtained for differential and MMP-12+-macrophages count analysis.

Results: The number of IS neutrophils was higher in both COPD groups compared to both controls. The amount of BAL neutrophils was higher in COPD smokers compared to healthy never-smokers. The number of BAL MMP-12+-macrophages was higher in COPD smokers (1.6 +/- 0.3 x 106/ml) compared to COPD ex-smokers, 'healthy' smokers and healthy never-smokers (0.9 +/- 0.4, 0.4 +/- 0.2, 0.2 +/- 0.1 x 106/ml respectively, p < 0.05).

Conclusion: The lower amount of BAL neutrophils in COPD ex-smokers, compared to COPD smokers, suggests positive alterations in alveolar compartment after smoking cessation. Smoking and disease itself may stimulate MMP-12 expression in airway compartments (IS and BAL) from COPD patients.

Show MeSH
Related in: MedlinePlus