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In earlier work, D'Andrea's team found that if monoubiquitination of FANCD2 is prevented, then the protein doesn't aggregate with BRCA1 and DNA repair is stymied, thus producing FA symptoms... D'Andrea has now found that mutation of FANCD2 phosphorylation sites has a very different effect... Thus the FANCD2 protein may integrate inputs from the two DNA repair pathways... Under normal circumstances, wild-type PA binds to a host receptor protein on the cell surface, initiating assembly of seven PA molecules into a ring... Beaudouin's new model, as he presented it at the meeting, is a departure from the two models previously put forward to explain NEBD... The mechanism underlying one of those models—phosphorylation-dependent depolymerization of nuclear lamins—may take over once the nucleus is breached, but Beaudouin did not see significant release of lamins before the large hole opened up... Reference: Actin filaments start from seeds, and those seeds are formed by complexes containing the actin-related Arp2/3 proteins... These mutant proteasomes degrade proteins ∼10-fold faster than wild-type proteasomes, but the mutant yeast don't show any growth defects—at least not until they are exposed to starvation conditions.If yeast cultures are starved for more than about a week, the viability of the proteasome mutants begins to fall off rapidly, whereas the wild-type cells are unaffected... Glickman found that wild-type cells react to starvation by shutting down all protein degradation, whereas the open-gate mutants continue to break down their proteins, a trait that eventually kills them... But, says Brown, the biochemistry “doesn't give you any real idea of what the rafts look like... In vitro people saw 1-μm sheets, and jumped to the conclusion that this is what rafts look like... Diffusion was extremely rapid, and different raft proteins diffused at different rates... Kenworthy concludes that raft proteins must exist in a variety of dynamically partitioning, rather than stable, structures.Kusumi is attempting to put more exact limits on the size and behavior of rafts by tracking beads that are attached to individual raft proteins... Kusumi notes, however, that these properties may change in stimulated cells when signaling complexes are more active... Reference:

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ErbB2 makes mammary acini (left) go haywire (right).Brugge/Macmillan
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uro3: ErbB2 makes mammary acini (left) go haywire (right).Brugge/Macmillan


Cells and More Cells
ErbB2 makes mammary acini (left) go haywire (right).Brugge/Macmillan
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Related In: Results  -  Collection

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uro3: ErbB2 makes mammary acini (left) go haywire (right).Brugge/Macmillan

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

In earlier work, D'Andrea's team found that if monoubiquitination of FANCD2 is prevented, then the protein doesn't aggregate with BRCA1 and DNA repair is stymied, thus producing FA symptoms... D'Andrea has now found that mutation of FANCD2 phosphorylation sites has a very different effect... Thus the FANCD2 protein may integrate inputs from the two DNA repair pathways... Under normal circumstances, wild-type PA binds to a host receptor protein on the cell surface, initiating assembly of seven PA molecules into a ring... Beaudouin's new model, as he presented it at the meeting, is a departure from the two models previously put forward to explain NEBD... The mechanism underlying one of those models—phosphorylation-dependent depolymerization of nuclear lamins—may take over once the nucleus is breached, but Beaudouin did not see significant release of lamins before the large hole opened up... Reference: Actin filaments start from seeds, and those seeds are formed by complexes containing the actin-related Arp2/3 proteins... These mutant proteasomes degrade proteins ∼10-fold faster than wild-type proteasomes, but the mutant yeast don't show any growth defects—at least not until they are exposed to starvation conditions.If yeast cultures are starved for more than about a week, the viability of the proteasome mutants begins to fall off rapidly, whereas the wild-type cells are unaffected... Glickman found that wild-type cells react to starvation by shutting down all protein degradation, whereas the open-gate mutants continue to break down their proteins, a trait that eventually kills them... But, says Brown, the biochemistry “doesn't give you any real idea of what the rafts look like... In vitro people saw 1-μm sheets, and jumped to the conclusion that this is what rafts look like... Diffusion was extremely rapid, and different raft proteins diffused at different rates... Kenworthy concludes that raft proteins must exist in a variety of dynamically partitioning, rather than stable, structures.Kusumi is attempting to put more exact limits on the size and behavior of rafts by tracking beads that are attached to individual raft proteins... Kusumi notes, however, that these properties may change in stimulated cells when signaling complexes are more active... Reference:

No MeSH data available.