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TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor.

Wong BR, Josien R, Lee SY, Sauter B, Li HL, Steinman RM, Choi Y - J. Exp. Med. (1997)

Bottom Line: Here, we show that high levels of TRANCE-R are detected on mature dendritic cells (DCs) but not on freshly isolated B cells, T cells, or macrophages.The resulting increase in DC survival is accompanied by a proportional increase in DC-mediated T cell proliferation in a mixed leukocyte reaction.TRANCE does not induce the proliferation of or increase the survival of T or B cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunology, The Rockefeller University, New York 10021, USA.

ABSTRACT
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine) is a new member of the TNF family that is induced upon T cell receptor engagement and activates c-Jun N-terminal kinase (JNK) after interaction with its putative receptor (TRANCE-R). In addition, TRANCE expression is restricted to lymphoid organs and T cells. Here, we show that high levels of TRANCE-R are detected on mature dendritic cells (DCs) but not on freshly isolated B cells, T cells, or macrophages. Signaling by TRANCE-R appears to be dependent on TNF receptor-associated factor 2 (TRAF2), since JNK induction is impaired in cells from transgenic mice overexpressing a dominant negative TRAF2 protein. TRANCE inhibits apoptosis of mouse bone marrow-derived DCs and human monocyte-derived DCs in vitro. The resulting increase in DC survival is accompanied by a proportional increase in DC-mediated T cell proliferation in a mixed leukocyte reaction. TRANCE upregulates Bcl-xL expression, suggesting a potential mechanism for enhanced DC survival. TRANCE does not induce the proliferation of or increase the survival of T or B cells. Therefore, TRANCE is a new DC-restricted survival factor that mediates T cell-DC communication and may provide a tool to selectively enhance DC activity.

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TRANCE-R expression in various cell types. Cells were prepared as described in the Materials and Methods section and stained with 10  μg/ml of the hCD8-TRANCE recombinant protein (solid lines) or with secondary reagents alone (dotted line). Only viable cells as determined by propidium iodide (PI) exclusion were gated and analyzed for TRANCE-R expression. Fresh DCs were analyzed by two-color staining after gating on  CD11chigh cells. Each staining was reproduced at least twice.
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Figure 1: TRANCE-R expression in various cell types. Cells were prepared as described in the Materials and Methods section and stained with 10 μg/ml of the hCD8-TRANCE recombinant protein (solid lines) or with secondary reagents alone (dotted line). Only viable cells as determined by propidium iodide (PI) exclusion were gated and analyzed for TRANCE-R expression. Fresh DCs were analyzed by two-color staining after gating on CD11chigh cells. Each staining was reproduced at least twice.

Mentions: To identify cells that express TRANCE-R, hCD8-TRANCE was used as a molecular probe for FACS® analysis. TRANCE-R was detected on mature BMDCs, freshly isolated lymph node DCs, and freshly isolated spleen DCs (Fig. 1). TRANCE-R was greatly upregulated upon the maturation of spleen DCs induced by overnight culture. No expression could be detected on freshly isolated lymph node B cells, lymph node T cells, thymocytes, or peritoneal macrophages. Therefore, the highest levels of TRANCE-R expression are found on mature DCs and suggest that the major role of TRANCE is restricted to DCs.


TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor.

Wong BR, Josien R, Lee SY, Sauter B, Li HL, Steinman RM, Choi Y - J. Exp. Med. (1997)

TRANCE-R expression in various cell types. Cells were prepared as described in the Materials and Methods section and stained with 10  μg/ml of the hCD8-TRANCE recombinant protein (solid lines) or with secondary reagents alone (dotted line). Only viable cells as determined by propidium iodide (PI) exclusion were gated and analyzed for TRANCE-R expression. Fresh DCs were analyzed by two-color staining after gating on  CD11chigh cells. Each staining was reproduced at least twice.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199171&req=5

Figure 1: TRANCE-R expression in various cell types. Cells were prepared as described in the Materials and Methods section and stained with 10 μg/ml of the hCD8-TRANCE recombinant protein (solid lines) or with secondary reagents alone (dotted line). Only viable cells as determined by propidium iodide (PI) exclusion were gated and analyzed for TRANCE-R expression. Fresh DCs were analyzed by two-color staining after gating on CD11chigh cells. Each staining was reproduced at least twice.
Mentions: To identify cells that express TRANCE-R, hCD8-TRANCE was used as a molecular probe for FACS® analysis. TRANCE-R was detected on mature BMDCs, freshly isolated lymph node DCs, and freshly isolated spleen DCs (Fig. 1). TRANCE-R was greatly upregulated upon the maturation of spleen DCs induced by overnight culture. No expression could be detected on freshly isolated lymph node B cells, lymph node T cells, thymocytes, or peritoneal macrophages. Therefore, the highest levels of TRANCE-R expression are found on mature DCs and suggest that the major role of TRANCE is restricted to DCs.

Bottom Line: Here, we show that high levels of TRANCE-R are detected on mature dendritic cells (DCs) but not on freshly isolated B cells, T cells, or macrophages.The resulting increase in DC survival is accompanied by a proportional increase in DC-mediated T cell proliferation in a mixed leukocyte reaction.TRANCE does not induce the proliferation of or increase the survival of T or B cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Immunology, The Rockefeller University, New York 10021, USA.

ABSTRACT
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine) is a new member of the TNF family that is induced upon T cell receptor engagement and activates c-Jun N-terminal kinase (JNK) after interaction with its putative receptor (TRANCE-R). In addition, TRANCE expression is restricted to lymphoid organs and T cells. Here, we show that high levels of TRANCE-R are detected on mature dendritic cells (DCs) but not on freshly isolated B cells, T cells, or macrophages. Signaling by TRANCE-R appears to be dependent on TNF receptor-associated factor 2 (TRAF2), since JNK induction is impaired in cells from transgenic mice overexpressing a dominant negative TRAF2 protein. TRANCE inhibits apoptosis of mouse bone marrow-derived DCs and human monocyte-derived DCs in vitro. The resulting increase in DC survival is accompanied by a proportional increase in DC-mediated T cell proliferation in a mixed leukocyte reaction. TRANCE upregulates Bcl-xL expression, suggesting a potential mechanism for enhanced DC survival. TRANCE does not induce the proliferation of or increase the survival of T or B cells. Therefore, TRANCE is a new DC-restricted survival factor that mediates T cell-DC communication and may provide a tool to selectively enhance DC activity.

Show MeSH
Related in: MedlinePlus