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Distinct roles of lymphotoxin alpha and the type I tumor necrosis factor (TNF) receptor in the establishment of follicular dendritic cells from non-bone marrow-derived cells.

Matsumoto M, Fu YX, Molina H, Huang G, Kim J, Thomas DA, Nahm MH, Chaplin DD - J. Exp. Med. (1997)

Bottom Line: Thus, expression of LT-alpha in the BM-derived cells, but not in the non-BM-derived cells, is required for the maturation of FDC from non-BM precursor cells.This indicates that TNFR-I expression on non-BM-derived cellular components is necessary for the establishment of these lymphoid structures.The data in this study demonstrate that FDC organization and GC formation are controlled by both LT-alpha-expressing BM-derived cells and by TNFR-I-expressing non-BM-derived cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Immunology and the Department of, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

ABSTRACT
In mice deficient in either lymphotoxin alpha (LT-alpha) or type I tumor necrosis factor receptor (TNFR-I), organized clusters of follicular dendritic cells (FDC) and germinal centers (GC) are absent from the spleen. We investigated the role of LT-alpha and TNFR-I in the establishment of spleen FDC and GC structure by using reciprocal bone marrow (BM) transfer. When LT-alpha-deficient mice were reconstituted with wild-type BM, FDC organization and the ability to form GC were restored, indicating that the LT-alpha-expressing cells required to establish organized FDC are derived from BM. The role of LT-alpha in establishing organized FDC structure was further investigated by the transfer of complement receptor 1 and 2 (CR1/2)-deficient BM cells into LT-alpha-deficient mice. Organized FDC were identified with both the FDC-M1 and anti-CR1 monoclonal antibodies in these BM-chimeric mice, indicating that these cells were derived from the LT-alpha-deficient recipient. Thus, expression of LT-alpha in the BM-derived cells, but not in the non-BM-derived cells, is required for the maturation of FDC from non-BM precursor cells. In contrast, when TNFR-I-deficient mice were reconstituted with wild-type BM, they showed no detectable FDC clusters or GC formation. This indicates that TNFR-I expression on non-BM-derived cellular components is necessary for the establishment of these lymphoid structures. TNFR-I-deficient BM was able to restore FDC organization and GC formation in LT-alpha-deficient mice, indicating that formation of these structures does not require TNFR-I expression on BM-derived cells. The data in this study demonstrate that FDC organization and GC formation are controlled by both LT-alpha-expressing BM-derived cells and by TNFR-I-expressing non-BM-derived cells.

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Restoration of organized FDC clusters and GC formation in LT-α–deficient mice reconstituted with TNFR-I–deficient BM. After BM  transfer, mice were immunized and spleens were harvested as described in Fig. 1. Serial sections are shown. (A) Staining was with anti-CR1 (blue) and  anti-B220 (brown). (B) Staining is with PNA (blue) and anti-B220 (brown). Original magnification, ×100.
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Figure 4: Restoration of organized FDC clusters and GC formation in LT-α–deficient mice reconstituted with TNFR-I–deficient BM. After BM transfer, mice were immunized and spleens were harvested as described in Fig. 1. Serial sections are shown. (A) Staining was with anti-CR1 (blue) and anti-B220 (brown). (B) Staining is with PNA (blue) and anti-B220 (brown). Original magnification, ×100.

Mentions: These data demonstrate that LT-α produced by BM-derived cells is required for the organization of FDC clusters, but that expression of LT-α is not required by the FDC themselves or their precursors. In contrast, the development of FDC clusters requires expression of TNFR-I on non–BM-derived cells, but not on BM-derived cells. Thus, clustering of FDC within spleen lymphoid follicles is controlled by at least two distinct cell populations, one defined by the expression of LT-α and the other defined by the expression of TNFR-I. To confirm that the requirement for LT-α and TNFR-I was a manifestation of the need for at least two distinct cell populations, we transferred BM cells from TNFR-I deficient donor mice (LT-α wild-type) into irradiated LT-α–deficient recipients (TNFR-I wild-type). Organized FDC clusters and the ability to form GC were demonstrated in these mice (Fig. 4, A and B). These results confirmed that neither expression of TNFR-I on the BM-derived cells nor of LT-α by radioresistant cells in the recipient is necessary for the development of FDC clusters or GC.


Distinct roles of lymphotoxin alpha and the type I tumor necrosis factor (TNF) receptor in the establishment of follicular dendritic cells from non-bone marrow-derived cells.

Matsumoto M, Fu YX, Molina H, Huang G, Kim J, Thomas DA, Nahm MH, Chaplin DD - J. Exp. Med. (1997)

Restoration of organized FDC clusters and GC formation in LT-α–deficient mice reconstituted with TNFR-I–deficient BM. After BM  transfer, mice were immunized and spleens were harvested as described in Fig. 1. Serial sections are shown. (A) Staining was with anti-CR1 (blue) and  anti-B220 (brown). (B) Staining is with PNA (blue) and anti-B220 (brown). Original magnification, ×100.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199170&req=5

Figure 4: Restoration of organized FDC clusters and GC formation in LT-α–deficient mice reconstituted with TNFR-I–deficient BM. After BM transfer, mice were immunized and spleens were harvested as described in Fig. 1. Serial sections are shown. (A) Staining was with anti-CR1 (blue) and anti-B220 (brown). (B) Staining is with PNA (blue) and anti-B220 (brown). Original magnification, ×100.
Mentions: These data demonstrate that LT-α produced by BM-derived cells is required for the organization of FDC clusters, but that expression of LT-α is not required by the FDC themselves or their precursors. In contrast, the development of FDC clusters requires expression of TNFR-I on non–BM-derived cells, but not on BM-derived cells. Thus, clustering of FDC within spleen lymphoid follicles is controlled by at least two distinct cell populations, one defined by the expression of LT-α and the other defined by the expression of TNFR-I. To confirm that the requirement for LT-α and TNFR-I was a manifestation of the need for at least two distinct cell populations, we transferred BM cells from TNFR-I deficient donor mice (LT-α wild-type) into irradiated LT-α–deficient recipients (TNFR-I wild-type). Organized FDC clusters and the ability to form GC were demonstrated in these mice (Fig. 4, A and B). These results confirmed that neither expression of TNFR-I on the BM-derived cells nor of LT-α by radioresistant cells in the recipient is necessary for the development of FDC clusters or GC.

Bottom Line: Thus, expression of LT-alpha in the BM-derived cells, but not in the non-BM-derived cells, is required for the maturation of FDC from non-BM precursor cells.This indicates that TNFR-I expression on non-BM-derived cellular components is necessary for the establishment of these lymphoid structures.The data in this study demonstrate that FDC organization and GC formation are controlled by both LT-alpha-expressing BM-derived cells and by TNFR-I-expressing non-BM-derived cells.

View Article: PubMed Central - PubMed

Affiliation: Center for Immunology and the Department of, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

ABSTRACT
In mice deficient in either lymphotoxin alpha (LT-alpha) or type I tumor necrosis factor receptor (TNFR-I), organized clusters of follicular dendritic cells (FDC) and germinal centers (GC) are absent from the spleen. We investigated the role of LT-alpha and TNFR-I in the establishment of spleen FDC and GC structure by using reciprocal bone marrow (BM) transfer. When LT-alpha-deficient mice were reconstituted with wild-type BM, FDC organization and the ability to form GC were restored, indicating that the LT-alpha-expressing cells required to establish organized FDC are derived from BM. The role of LT-alpha in establishing organized FDC structure was further investigated by the transfer of complement receptor 1 and 2 (CR1/2)-deficient BM cells into LT-alpha-deficient mice. Organized FDC were identified with both the FDC-M1 and anti-CR1 monoclonal antibodies in these BM-chimeric mice, indicating that these cells were derived from the LT-alpha-deficient recipient. Thus, expression of LT-alpha in the BM-derived cells, but not in the non-BM-derived cells, is required for the maturation of FDC from non-BM precursor cells. In contrast, when TNFR-I-deficient mice were reconstituted with wild-type BM, they showed no detectable FDC clusters or GC formation. This indicates that TNFR-I expression on non-BM-derived cellular components is necessary for the establishment of these lymphoid structures. TNFR-I-deficient BM was able to restore FDC organization and GC formation in LT-alpha-deficient mice, indicating that formation of these structures does not require TNFR-I expression on BM-derived cells. The data in this study demonstrate that FDC organization and GC formation are controlled by both LT-alpha-expressing BM-derived cells and by TNFR-I-expressing non-BM-derived cells.

Show MeSH
Related in: MedlinePlus