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Evidence that singlet oxygen-induced human T helper cell apoptosis is the basic mechanism of ultraviolet-A radiation phototherapy.

Morita A, Werfel T, Stege H, Ahrens C, Karmann K, Grewe M, Grether-Beck S, Ruzicka T, Kapp A, Klotz LO, Sies H, Krutmann J - J. Exp. Med. (1997)

Bottom Line: In the present study, successful phototherapy of atopic dermatitis was found to result from UVA radiation-induced apoptosis in skin-infiltrating T helper cells, leading to T cell depletion from eczematous skin.In vitro, UVA radiation-induced human T helper cell apoptosis was mediated through the FAS/FAS-ligand system, which was activated in irradiated T cells as a consequence of singlet oxygen generation.These studies demonstrate that singlet oxygen is a potent trigger for the induction of human T cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Clinical and Experimental Photodermatology, Department of Dermatology, Heinrich-Heine-University of Düsseldorf, Germany.

ABSTRACT
Ultraviolet A (UVA) irradiation is effectively used to treat patients with atopic dermatitis and other T cell mediated, inflammatory skin diseases. In the present study, successful phototherapy of atopic dermatitis was found to result from UVA radiation-induced apoptosis in skin-infiltrating T helper cells, leading to T cell depletion from eczematous skin. In vitro, UVA radiation-induced human T helper cell apoptosis was mediated through the FAS/FAS-ligand system, which was activated in irradiated T cells as a consequence of singlet oxygen generation. These studies demonstrate that singlet oxygen is a potent trigger for the induction of human T cell apoptosis. They also identify singlet oxygen generation as a fundamental mechanism of action operative in phototherapy.

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Related in: MedlinePlus

UVA radiation- induced apoptosis and FASL expression in human T cells. Human T helper cells were exposed  to increasing doses of UVA radiation (0–30 J/cm2) in the presence (open bar) or absence (solid  bar) of sodium azide (50 mM) or  deuterium oxide (90%) (hatched  bar). 4 h after irradiation, the  percentage of apoptotic cells (A)  and FASL+ cells (B) was determined as described in Materials  and Methods. Data represent one  of three essentially identical experiments.
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Figure 6: UVA radiation- induced apoptosis and FASL expression in human T cells. Human T helper cells were exposed to increasing doses of UVA radiation (0–30 J/cm2) in the presence (open bar) or absence (solid bar) of sodium azide (50 mM) or deuterium oxide (90%) (hatched bar). 4 h after irradiation, the percentage of apoptotic cells (A) and FASL+ cells (B) was determined as described in Materials and Methods. Data represent one of three essentially identical experiments.

Mentions: In atopen-specific human T helper cells, in vitro UVA irradiation induced apoptosis (Fig. 3, see also Figs. 5, 6). Significant apoptosis was already detectable 4 h after exposure, reaching a maximum 24 h after irradiation with 30 J/cm2 UVA radiation (Fig. 3 and data not shown).


Evidence that singlet oxygen-induced human T helper cell apoptosis is the basic mechanism of ultraviolet-A radiation phototherapy.

Morita A, Werfel T, Stege H, Ahrens C, Karmann K, Grewe M, Grether-Beck S, Ruzicka T, Kapp A, Klotz LO, Sies H, Krutmann J - J. Exp. Med. (1997)

UVA radiation- induced apoptosis and FASL expression in human T cells. Human T helper cells were exposed  to increasing doses of UVA radiation (0–30 J/cm2) in the presence (open bar) or absence (solid  bar) of sodium azide (50 mM) or  deuterium oxide (90%) (hatched  bar). 4 h after irradiation, the  percentage of apoptotic cells (A)  and FASL+ cells (B) was determined as described in Materials  and Methods. Data represent one  of three essentially identical experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199141&req=5

Figure 6: UVA radiation- induced apoptosis and FASL expression in human T cells. Human T helper cells were exposed to increasing doses of UVA radiation (0–30 J/cm2) in the presence (open bar) or absence (solid bar) of sodium azide (50 mM) or deuterium oxide (90%) (hatched bar). 4 h after irradiation, the percentage of apoptotic cells (A) and FASL+ cells (B) was determined as described in Materials and Methods. Data represent one of three essentially identical experiments.
Mentions: In atopen-specific human T helper cells, in vitro UVA irradiation induced apoptosis (Fig. 3, see also Figs. 5, 6). Significant apoptosis was already detectable 4 h after exposure, reaching a maximum 24 h after irradiation with 30 J/cm2 UVA radiation (Fig. 3 and data not shown).

Bottom Line: In the present study, successful phototherapy of atopic dermatitis was found to result from UVA radiation-induced apoptosis in skin-infiltrating T helper cells, leading to T cell depletion from eczematous skin.In vitro, UVA radiation-induced human T helper cell apoptosis was mediated through the FAS/FAS-ligand system, which was activated in irradiated T cells as a consequence of singlet oxygen generation.These studies demonstrate that singlet oxygen is a potent trigger for the induction of human T cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Clinical and Experimental Photodermatology, Department of Dermatology, Heinrich-Heine-University of Düsseldorf, Germany.

ABSTRACT
Ultraviolet A (UVA) irradiation is effectively used to treat patients with atopic dermatitis and other T cell mediated, inflammatory skin diseases. In the present study, successful phototherapy of atopic dermatitis was found to result from UVA radiation-induced apoptosis in skin-infiltrating T helper cells, leading to T cell depletion from eczematous skin. In vitro, UVA radiation-induced human T helper cell apoptosis was mediated through the FAS/FAS-ligand system, which was activated in irradiated T cells as a consequence of singlet oxygen generation. These studies demonstrate that singlet oxygen is a potent trigger for the induction of human T cell apoptosis. They also identify singlet oxygen generation as a fundamental mechanism of action operative in phototherapy.

Show MeSH
Related in: MedlinePlus