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Peripheral selection of T cell repertoires: the role of continuous thymus output.

Tanchot C, Rocha B - J. Exp. Med. (1997)

Bottom Line: We investigated the role of continuous thymus output in the shaping of mature T cell repertoires by studying in vivo the survival of a single clone of mature Rag2-deficient T cell receptor (TCR) transgenic cells at different stages of activation in the absence or presence of thymus export.This T cell renewal ensured both the efficiency of recall responses to antigens as memory T cells persisted independently of thymus output, and the capacity of the immune system to respond to new antigen stimulation as the naive T cell pool was continuously renewed.Our results also indicate that thymus export is required to control the number of self-reactive peripheral T cells that may invade the peripheral pools if thymus output fails.

View Article: PubMed Central - PubMed

Affiliation: U345 Institut National de la Sante et de la Recherche Medicale, Institut Necker, 156, 75730 Paris Cedex 15, France.

ABSTRACT
We investigated the role of continuous thymus output in the shaping of mature T cell repertoires by studying in vivo the survival of a single clone of mature Rag2-deficient T cell receptor (TCR) transgenic cells at different stages of activation in the absence or presence of thymus export. In the absence of thymus export, TCR-transgenic lymphocytes survived indefinitely in the peripheral pools. When new lymphocytes were produced in the thymus and migrated to the periphery, resident memory T cells were maintained in constant numbers, whereas naive and self-reactive T cells were replaced by recent thymus migrants. This T cell renewal ensured both the efficiency of recall responses to antigens as memory T cells persisted independently of thymus output, and the capacity of the immune system to respond to new antigen stimulation as the naive T cell pool was continuously renewed. Our results also indicate that thymus export is required to control the number of self-reactive peripheral T cells that may invade the peripheral pools if thymus output fails.

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Generation of male/female chimeric mice, with our without  thymus export. All male/female chimeras were injected with 0.5 × 106  Tg cells. (a) Tg cells remain functional. (b) Tg cells become tolerant.
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Figure 1: Generation of male/female chimeric mice, with our without thymus export. All male/female chimeras were injected with 0.5 × 106 Tg cells. (a) Tg cells remain functional. (b) Tg cells become tolerant.

Mentions: We devised protocols in which we could induce the same clone of T cells into different states of activation and then study the substitution of such clone by T cells migrating from the thymus. The protocols are summarized in Fig. 1.


Peripheral selection of T cell repertoires: the role of continuous thymus output.

Tanchot C, Rocha B - J. Exp. Med. (1997)

Generation of male/female chimeric mice, with our without  thymus export. All male/female chimeras were injected with 0.5 × 106  Tg cells. (a) Tg cells remain functional. (b) Tg cells become tolerant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199066&req=5

Figure 1: Generation of male/female chimeric mice, with our without thymus export. All male/female chimeras were injected with 0.5 × 106 Tg cells. (a) Tg cells remain functional. (b) Tg cells become tolerant.
Mentions: We devised protocols in which we could induce the same clone of T cells into different states of activation and then study the substitution of such clone by T cells migrating from the thymus. The protocols are summarized in Fig. 1.

Bottom Line: We investigated the role of continuous thymus output in the shaping of mature T cell repertoires by studying in vivo the survival of a single clone of mature Rag2-deficient T cell receptor (TCR) transgenic cells at different stages of activation in the absence or presence of thymus export.This T cell renewal ensured both the efficiency of recall responses to antigens as memory T cells persisted independently of thymus output, and the capacity of the immune system to respond to new antigen stimulation as the naive T cell pool was continuously renewed.Our results also indicate that thymus export is required to control the number of self-reactive peripheral T cells that may invade the peripheral pools if thymus output fails.

View Article: PubMed Central - PubMed

Affiliation: U345 Institut National de la Sante et de la Recherche Medicale, Institut Necker, 156, 75730 Paris Cedex 15, France.

ABSTRACT
We investigated the role of continuous thymus output in the shaping of mature T cell repertoires by studying in vivo the survival of a single clone of mature Rag2-deficient T cell receptor (TCR) transgenic cells at different stages of activation in the absence or presence of thymus export. In the absence of thymus export, TCR-transgenic lymphocytes survived indefinitely in the peripheral pools. When new lymphocytes were produced in the thymus and migrated to the periphery, resident memory T cells were maintained in constant numbers, whereas naive and self-reactive T cells were replaced by recent thymus migrants. This T cell renewal ensured both the efficiency of recall responses to antigens as memory T cells persisted independently of thymus output, and the capacity of the immune system to respond to new antigen stimulation as the naive T cell pool was continuously renewed. Our results also indicate that thymus export is required to control the number of self-reactive peripheral T cells that may invade the peripheral pools if thymus output fails.

Show MeSH
Related in: MedlinePlus