Limits...
Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.

Mueller CG, Rissoan MC, Salinas B, Ait-Yahia S, Ravel O, Bridon JM, Briere F, Lebecque S, Liu YJ - J. Exp. Med. (1997)

Bottom Line: Dendritic cell precursors or immature dendritic cells express no or low levels of the message.It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction.In situ hybridization showed distinct expression of this gene in the germinal center.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France.

ABSTRACT
To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

Show MeSH

Related in: MedlinePlus

Localization of decysin mRNA to germinal centers by in situ hybridization. Human tonsil sections are hybridized with antisense (A, C) and  sense (B) decysin 35S-labeled RNA probes. (A) and (B) are from serial sections. Original magnification: 40. The germinal center marked by an arrow original magnification: 100 (C). The sense probe generates diffuse background hybridization, whereas with the antisense probe, dense clusters of silver grains  are seen mainly localized to the follicles. D is from reference 4, and shows immunohistological staining of CD4+CD11c+ GCDCs with anti-CD11c (red). In  blue are proliferating cells stained by anti-Ki67. Original magnification: 100.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2199019&req=5

Figure 6: Localization of decysin mRNA to germinal centers by in situ hybridization. Human tonsil sections are hybridized with antisense (A, C) and sense (B) decysin 35S-labeled RNA probes. (A) and (B) are from serial sections. Original magnification: 40. The germinal center marked by an arrow original magnification: 100 (C). The sense probe generates diffuse background hybridization, whereas with the antisense probe, dense clusters of silver grains are seen mainly localized to the follicles. D is from reference 4, and shows immunohistological staining of CD4+CD11c+ GCDCs with anti-CD11c (red). In blue are proliferating cells stained by anti-Ki67. Original magnification: 100.

Mentions: To localize decysin mRNA in human lymph nodes, in situ hybridization was performed (Fig. 6). A tonsil section probed with the antisense RNA strand shows distinct hybridization signals primarily within germinal centers (A and C) in contrast to the same follicles probed with the sense strand (B). The follicle marked by an arrow is shown in higher magnification (C). Silver grains are in focalized clusters evenly distributed with the germinal center. This profile is identical to that obtained by anti-CD11c immunostaining of GCDCs (compare C and D), and confirms that DCs of the germinal center express high levels of decysin.


Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.

Mueller CG, Rissoan MC, Salinas B, Ait-Yahia S, Ravel O, Bridon JM, Briere F, Lebecque S, Liu YJ - J. Exp. Med. (1997)

Localization of decysin mRNA to germinal centers by in situ hybridization. Human tonsil sections are hybridized with antisense (A, C) and  sense (B) decysin 35S-labeled RNA probes. (A) and (B) are from serial sections. Original magnification: 40. The germinal center marked by an arrow original magnification: 100 (C). The sense probe generates diffuse background hybridization, whereas with the antisense probe, dense clusters of silver grains  are seen mainly localized to the follicles. D is from reference 4, and shows immunohistological staining of CD4+CD11c+ GCDCs with anti-CD11c (red). In  blue are proliferating cells stained by anti-Ki67. Original magnification: 100.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199019&req=5

Figure 6: Localization of decysin mRNA to germinal centers by in situ hybridization. Human tonsil sections are hybridized with antisense (A, C) and sense (B) decysin 35S-labeled RNA probes. (A) and (B) are from serial sections. Original magnification: 40. The germinal center marked by an arrow original magnification: 100 (C). The sense probe generates diffuse background hybridization, whereas with the antisense probe, dense clusters of silver grains are seen mainly localized to the follicles. D is from reference 4, and shows immunohistological staining of CD4+CD11c+ GCDCs with anti-CD11c (red). In blue are proliferating cells stained by anti-Ki67. Original magnification: 100.
Mentions: To localize decysin mRNA in human lymph nodes, in situ hybridization was performed (Fig. 6). A tonsil section probed with the antisense RNA strand shows distinct hybridization signals primarily within germinal centers (A and C) in contrast to the same follicles probed with the sense strand (B). The follicle marked by an arrow is shown in higher magnification (C). Silver grains are in focalized clusters evenly distributed with the germinal center. This profile is identical to that obtained by anti-CD11c immunostaining of GCDCs (compare C and D), and confirms that DCs of the germinal center express high levels of decysin.

Bottom Line: Dendritic cell precursors or immature dendritic cells express no or low levels of the message.It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction.In situ hybridization showed distinct expression of this gene in the germinal center.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France.

ABSTRACT
To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

Show MeSH
Related in: MedlinePlus