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Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.

Mueller CG, Rissoan MC, Salinas B, Ait-Yahia S, Ravel O, Bridon JM, Briere F, Lebecque S, Liu YJ - J. Exp. Med. (1997)

Bottom Line: It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction.In situ hybridization showed distinct expression of this gene in the germinal center.This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France.

ABSTRACT
To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

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Related in: MedlinePlus

Northern analysis. PolyA+ RNA from different human tissues was hybridized with a specific decysin probe. Shown here are multiple tissue blot II (No. 7759-1; Clontech) and immune systems blot (No.  7754-1; Clontech). Not shown are tissue blots No. 7760-1 and No.  7756-1 as they were completely negative. Exposure time was 2 wk.
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Figure 5: Northern analysis. PolyA+ RNA from different human tissues was hybridized with a specific decysin probe. Shown here are multiple tissue blot II (No. 7759-1; Clontech) and immune systems blot (No. 7754-1; Clontech). Not shown are tissue blots No. 7760-1 and No. 7756-1 as they were completely negative. Exposure time was 2 wk.

Mentions: By Northern blot analysis on different human tissues (Fig. 5), decysin is expressed as a single 2.4-kb message and is highly abundant in the small intestine and appendix. Database searches produced a single partial expressed sequencing tag from pig small intestine (not shown). Expression is also seen in lymph node, mucosal lining of the colon, thymus, spleen, and very weakly in bone marrow. Peripheral blood, ovary, testis, prostate, and fetal liver are negative, as well as other blots containing tissues such as heart, lung, or liver (data not shown).


Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.

Mueller CG, Rissoan MC, Salinas B, Ait-Yahia S, Ravel O, Bridon JM, Briere F, Lebecque S, Liu YJ - J. Exp. Med. (1997)

Northern analysis. PolyA+ RNA from different human tissues was hybridized with a specific decysin probe. Shown here are multiple tissue blot II (No. 7759-1; Clontech) and immune systems blot (No.  7754-1; Clontech). Not shown are tissue blots No. 7760-1 and No.  7756-1 as they were completely negative. Exposure time was 2 wk.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199019&req=5

Figure 5: Northern analysis. PolyA+ RNA from different human tissues was hybridized with a specific decysin probe. Shown here are multiple tissue blot II (No. 7759-1; Clontech) and immune systems blot (No. 7754-1; Clontech). Not shown are tissue blots No. 7760-1 and No. 7756-1 as they were completely negative. Exposure time was 2 wk.
Mentions: By Northern blot analysis on different human tissues (Fig. 5), decysin is expressed as a single 2.4-kb message and is highly abundant in the small intestine and appendix. Database searches produced a single partial expressed sequencing tag from pig small intestine (not shown). Expression is also seen in lymph node, mucosal lining of the colon, thymus, spleen, and very weakly in bone marrow. Peripheral blood, ovary, testis, prostate, and fetal liver are negative, as well as other blots containing tissues such as heart, lung, or liver (data not shown).

Bottom Line: It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction.In situ hybridization showed distinct expression of this gene in the germinal center.This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

View Article: PubMed Central - PubMed

Affiliation: Schering-Plough Laboratory for Immunological Research, 69571 Dardilly, France.

ABSTRACT
To identify genes expressed by a specific subset of dendritic cells found in vivo a polymerase chain reaction-based cDNA subtraction technique was applied to the recently described germinal center dendritic cells. A novel member of the disintegrin metalloproteinase family was cloned which comprises a not typical zinc-chelating catalytic site most similar to a bacterial metalloproteinase. Dendritic cell precursors or immature dendritic cells express no or low levels of the message. It is induced to high levels upon spontaneous or CD40-dependent maturation and in a mixed lymphocyte reaction. In situ hybridization showed distinct expression of this gene in the germinal center. This, together with the findings that certain disintegrin metalloproteinases regulate the activity of tumor necrosis factor alpha and that metalloproteinases have also been implicated in FasL processing, suggest that this novel molecule may play an important role in dendritic cell function and their interactions with germinal center T cells.

Show MeSH
Related in: MedlinePlus