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Disruption of the Bcl6 gene results in an impaired germinal center formation.

Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, Okabe S, Koseki H, Hirosawa S, Taniguchi M, Miyasaka N, Tokuhisa T - J. Exp. Med. (1997)

Bottom Line: Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens.This defect was mainly due to the abnormalities of B cells.Therefore, Bcl6 is essential for the differentiation of GC B cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Genetics, Center for Biomedical Science, Chiba University School of Medicine, Chiba, Japan.

ABSTRACT
The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens. However, GCs were not found in these mice. This defect was mainly due to the abnormalities of B cells. Therefore, Bcl6 is essential for the differentiation of GC B cells.

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Nascent GC is absent in spleen from Bcl6−/−RM. Spleen  sections from Bcl6+/+RM (A) and Bcl6−/−RM (B) on day 3 after immunization were stained with anti-BrdU Ab and PNA. Strong blue signals in  the nuclei and membranous weak blue signals indicate BrdU uptake and  cell surface Igs, respectively. Brown signals in follicle of Bcl6+/+RM indicate PNA-reactive GC cells.
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Figure 7: Nascent GC is absent in spleen from Bcl6−/−RM. Spleen sections from Bcl6+/+RM (A) and Bcl6−/−RM (B) on day 3 after immunization were stained with anti-BrdU Ab and PNA. Strong blue signals in the nuclei and membranous weak blue signals indicate BrdU uptake and cell surface Igs, respectively. Brown signals in follicle of Bcl6+/+RM indicate PNA-reactive GC cells.

Mentions: GC first appears in splenic follicles by day 4 and begins to wane by day 21 after immunization (31). It might be possible that GC formation occurs in Bcl6−/−RM shortly after immunization but cannot be maintained. To examine the possibility, the presence of PNA-binding GCs was examined in spleens from Bcl6−/−RM immunized with DNP– OVA at an early stage after immunization. Nascent PNA-binding GCs could be identified in the Bcl6+/+RM spleen on day 3 after immunization (Fig. 7 A) and Bcl6 was detected strongly in nuclei of these GC B cells (data not shown). However, the nascent GCs were not detected at all in the Bcl6−/−RM on day 3 (Fig. 7 B) and day 4 (data not shown) after immunization.


Disruption of the Bcl6 gene results in an impaired germinal center formation.

Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, Okabe S, Koseki H, Hirosawa S, Taniguchi M, Miyasaka N, Tokuhisa T - J. Exp. Med. (1997)

Nascent GC is absent in spleen from Bcl6−/−RM. Spleen  sections from Bcl6+/+RM (A) and Bcl6−/−RM (B) on day 3 after immunization were stained with anti-BrdU Ab and PNA. Strong blue signals in  the nuclei and membranous weak blue signals indicate BrdU uptake and  cell surface Igs, respectively. Brown signals in follicle of Bcl6+/+RM indicate PNA-reactive GC cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199007&req=5

Figure 7: Nascent GC is absent in spleen from Bcl6−/−RM. Spleen sections from Bcl6+/+RM (A) and Bcl6−/−RM (B) on day 3 after immunization were stained with anti-BrdU Ab and PNA. Strong blue signals in the nuclei and membranous weak blue signals indicate BrdU uptake and cell surface Igs, respectively. Brown signals in follicle of Bcl6+/+RM indicate PNA-reactive GC cells.
Mentions: GC first appears in splenic follicles by day 4 and begins to wane by day 21 after immunization (31). It might be possible that GC formation occurs in Bcl6−/−RM shortly after immunization but cannot be maintained. To examine the possibility, the presence of PNA-binding GCs was examined in spleens from Bcl6−/−RM immunized with DNP– OVA at an early stage after immunization. Nascent PNA-binding GCs could be identified in the Bcl6+/+RM spleen on day 3 after immunization (Fig. 7 A) and Bcl6 was detected strongly in nuclei of these GC B cells (data not shown). However, the nascent GCs were not detected at all in the Bcl6−/−RM on day 3 (Fig. 7 B) and day 4 (data not shown) after immunization.

Bottom Line: Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens.This defect was mainly due to the abnormalities of B cells.Therefore, Bcl6 is essential for the differentiation of GC B cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Genetics, Center for Biomedical Science, Chiba University School of Medicine, Chiba, Japan.

ABSTRACT
The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens. However, GCs were not found in these mice. This defect was mainly due to the abnormalities of B cells. Therefore, Bcl6 is essential for the differentiation of GC B cells.

Show MeSH
Related in: MedlinePlus