Limits...
Disruption of the Bcl6 gene results in an impaired germinal center formation.

Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, Okabe S, Koseki H, Hirosawa S, Taniguchi M, Miyasaka N, Tokuhisa T - J. Exp. Med. (1997)

Bottom Line: Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens.This defect was mainly due to the abnormalities of B cells.Therefore, Bcl6 is essential for the differentiation of GC B cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Genetics, Center for Biomedical Science, Chiba University School of Medicine, Chiba, Japan.

ABSTRACT
The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens. However, GCs were not found in these mice. This defect was mainly due to the abnormalities of B cells. Therefore, Bcl6 is essential for the differentiation of GC B cells.

Show MeSH

Related in: MedlinePlus

Bcl6 expression in spleen from immunized C57BL/6. Spleen sections from C57BL/6 mice on day 14 (A and B) or day 7 (C and D) after immunization were stained with anti-Bcl6 Ab (A and B) or anti-Bcl6 and anti-λ light chain Abs (C and D). Brown signals in nuclei and membranous blue  signals indicate Bcl6 protein and λ light chain, respectively. Sections were counterstained with hematoxylin (A and B). Arrows and arrowheads indicate  centroblasts and centrocytes respectively.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2199007&req=5

Figure 1: Bcl6 expression in spleen from immunized C57BL/6. Spleen sections from C57BL/6 mice on day 14 (A and B) or day 7 (C and D) after immunization were stained with anti-Bcl6 Ab (A and B) or anti-Bcl6 and anti-λ light chain Abs (C and D). Brown signals in nuclei and membranous blue signals indicate Bcl6 protein and λ light chain, respectively. Sections were counterstained with hematoxylin (A and B). Arrows and arrowheads indicate centroblasts and centrocytes respectively.

Mentions: We have made polyclonal antibodies specific for mouse Bcl6 and immunohistologically analyzed the expression in GCs from spleens of immunized mice. The GC reaction in spleen reaches its maximum by days 10–12 and keeps the same high levels until day 21 after immunization (31, 36). Therefore, expression of Bcl6 was examined in spleens of normal C57BL/6 mice immunized with T cell– dependent antigen, DNP–OVA, on day 14 after immunization (Fig. 1, A and B). Bcl6 was detected strongly in nuclei of most of GC B cells and weakly in those of other lymphocytes in white pulps.


Disruption of the Bcl6 gene results in an impaired germinal center formation.

Fukuda T, Yoshida T, Okada S, Hatano M, Miki T, Ishibashi K, Okabe S, Koseki H, Hirosawa S, Taniguchi M, Miyasaka N, Tokuhisa T - J. Exp. Med. (1997)

Bcl6 expression in spleen from immunized C57BL/6. Spleen sections from C57BL/6 mice on day 14 (A and B) or day 7 (C and D) after immunization were stained with anti-Bcl6 Ab (A and B) or anti-Bcl6 and anti-λ light chain Abs (C and D). Brown signals in nuclei and membranous blue  signals indicate Bcl6 protein and λ light chain, respectively. Sections were counterstained with hematoxylin (A and B). Arrows and arrowheads indicate  centroblasts and centrocytes respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2199007&req=5

Figure 1: Bcl6 expression in spleen from immunized C57BL/6. Spleen sections from C57BL/6 mice on day 14 (A and B) or day 7 (C and D) after immunization were stained with anti-Bcl6 Ab (A and B) or anti-Bcl6 and anti-λ light chain Abs (C and D). Brown signals in nuclei and membranous blue signals indicate Bcl6 protein and λ light chain, respectively. Sections were counterstained with hematoxylin (A and B). Arrows and arrowheads indicate centroblasts and centrocytes respectively.
Mentions: We have made polyclonal antibodies specific for mouse Bcl6 and immunohistologically analyzed the expression in GCs from spleens of immunized mice. The GC reaction in spleen reaches its maximum by days 10–12 and keeps the same high levels until day 21 after immunization (31, 36). Therefore, expression of Bcl6 was examined in spleens of normal C57BL/6 mice immunized with T cell– dependent antigen, DNP–OVA, on day 14 after immunization (Fig. 1, A and B). Bcl6 was detected strongly in nuclei of most of GC B cells and weakly in those of other lymphocytes in white pulps.

Bottom Line: Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens.This defect was mainly due to the abnormalities of B cells.Therefore, Bcl6 is essential for the differentiation of GC B cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Developmental Genetics, Center for Biomedical Science, Chiba University School of Medicine, Chiba, Japan.

ABSTRACT
The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is normal, and Bcl6(-/-)RM produced control levels of primary IgG1 antibodies specific to T cell-dependent antigens. However, GCs were not found in these mice. This defect was mainly due to the abnormalities of B cells. Therefore, Bcl6 is essential for the differentiation of GC B cells.

Show MeSH
Related in: MedlinePlus