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Development of eosinophilic airway inflammation and airway hyperresponsiveness in mast cell-deficient mice.

Takeda K, Hamelmann E, Joetham A, Shultz LD, Larsen GL, Irvin CG, Gelfand EW - J. Exp. Med. (1997)

Bottom Line: Comparison of OVA-specific immunoglobulin E (IgE) levels in the serum and numbers of eosinophils in bronchoalveolar lavage fluid or lung digests showed no differences between the two groups of mice.Further, measurements of airway resistance and dynamic compliance at baseline and after inhalation of methacholine were similar.These data indicate that mast cells or IgE-mast cell activation is not required for the development of eosinophilic inflammation and AHR in mice sensitized to allergen via the intraperitoneal route and challenged via the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic Sciences and Pulmonary Medicine, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, USA.

ABSTRACT
Mast cells are the main effector cells of immediate hypersensitivity and anaphylaxis. Their role in the development of allergen-induced airway hyperresponsiveness (AHR) is controversial and based on indirect evidence. To address these issues, mast cell-deficient mice (W/W v) and their congenic littermates were sensitized to ovalbumin (OVA) by intraperitoneal injection and subsequently challenged with OVA via the airways. Comparison of OVA-specific immunoglobulin E (IgE) levels in the serum and numbers of eosinophils in bronchoalveolar lavage fluid or lung digests showed no differences between the two groups of mice. Further, measurements of airway resistance and dynamic compliance at baseline and after inhalation of methacholine were similar. These data indicate that mast cells or IgE-mast cell activation is not required for the development of eosinophilic inflammation and AHR in mice sensitized to allergen via the intraperitoneal route and challenged via the airways.

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(a) Cellular composition of BAL fluid. Mice were sensitized  and challenged as described in Materials and Methods. BAL fluid was obtained from the same groups described in the legend to Fig. 1. The results  for each group are expressed as means ± SEM. *Significant differences  (P <0.05) between the groups (N versus IpN). (b) Cellular composition  of isolated lung cells. Lung cells were prepared from animals sensitized  and challenged as described in the legend to Fig. 1. The results for each  group are expressed as means ± SEM (n = 4/group). *Significant differences (P <0.05) between the groups (N versus IpN).
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Figure 2: (a) Cellular composition of BAL fluid. Mice were sensitized and challenged as described in Materials and Methods. BAL fluid was obtained from the same groups described in the legend to Fig. 1. The results for each group are expressed as means ± SEM. *Significant differences (P <0.05) between the groups (N versus IpN). (b) Cellular composition of isolated lung cells. Lung cells were prepared from animals sensitized and challenged as described in the legend to Fig. 1. The results for each group are expressed as means ± SEM (n = 4/group). *Significant differences (P <0.05) between the groups (N versus IpN).

Mentions: As shown in Fig. 2 a, sensitization and challenge with OVA had a marked effect on the numbers and composition of the cells recovered. In both groups of mice, macrophages were the predominant cell type in the mice receiving three challenges with OVA alone (similar to control mice; data not shown). However, after both sensitization and challenge, cell numbers increased and the predominant cells in the BAL were eosinophils, comprising roughly 60% of the cells in both the mast cell–deficient and congenic littermates.


Development of eosinophilic airway inflammation and airway hyperresponsiveness in mast cell-deficient mice.

Takeda K, Hamelmann E, Joetham A, Shultz LD, Larsen GL, Irvin CG, Gelfand EW - J. Exp. Med. (1997)

(a) Cellular composition of BAL fluid. Mice were sensitized  and challenged as described in Materials and Methods. BAL fluid was obtained from the same groups described in the legend to Fig. 1. The results  for each group are expressed as means ± SEM. *Significant differences  (P <0.05) between the groups (N versus IpN). (b) Cellular composition  of isolated lung cells. Lung cells were prepared from animals sensitized  and challenged as described in the legend to Fig. 1. The results for each  group are expressed as means ± SEM (n = 4/group). *Significant differences (P <0.05) between the groups (N versus IpN).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2198995&req=5

Figure 2: (a) Cellular composition of BAL fluid. Mice were sensitized and challenged as described in Materials and Methods. BAL fluid was obtained from the same groups described in the legend to Fig. 1. The results for each group are expressed as means ± SEM. *Significant differences (P <0.05) between the groups (N versus IpN). (b) Cellular composition of isolated lung cells. Lung cells were prepared from animals sensitized and challenged as described in the legend to Fig. 1. The results for each group are expressed as means ± SEM (n = 4/group). *Significant differences (P <0.05) between the groups (N versus IpN).
Mentions: As shown in Fig. 2 a, sensitization and challenge with OVA had a marked effect on the numbers and composition of the cells recovered. In both groups of mice, macrophages were the predominant cell type in the mice receiving three challenges with OVA alone (similar to control mice; data not shown). However, after both sensitization and challenge, cell numbers increased and the predominant cells in the BAL were eosinophils, comprising roughly 60% of the cells in both the mast cell–deficient and congenic littermates.

Bottom Line: Comparison of OVA-specific immunoglobulin E (IgE) levels in the serum and numbers of eosinophils in bronchoalveolar lavage fluid or lung digests showed no differences between the two groups of mice.Further, measurements of airway resistance and dynamic compliance at baseline and after inhalation of methacholine were similar.These data indicate that mast cells or IgE-mast cell activation is not required for the development of eosinophilic inflammation and AHR in mice sensitized to allergen via the intraperitoneal route and challenged via the airways.

View Article: PubMed Central - PubMed

Affiliation: Division of Basic Sciences and Pulmonary Medicine, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado, USA.

ABSTRACT
Mast cells are the main effector cells of immediate hypersensitivity and anaphylaxis. Their role in the development of allergen-induced airway hyperresponsiveness (AHR) is controversial and based on indirect evidence. To address these issues, mast cell-deficient mice (W/W v) and their congenic littermates were sensitized to ovalbumin (OVA) by intraperitoneal injection and subsequently challenged with OVA via the airways. Comparison of OVA-specific immunoglobulin E (IgE) levels in the serum and numbers of eosinophils in bronchoalveolar lavage fluid or lung digests showed no differences between the two groups of mice. Further, measurements of airway resistance and dynamic compliance at baseline and after inhalation of methacholine were similar. These data indicate that mast cells or IgE-mast cell activation is not required for the development of eosinophilic inflammation and AHR in mice sensitized to allergen via the intraperitoneal route and challenged via the airways.

Show MeSH
Related in: MedlinePlus